scholarly journals Morphologically and physiologically diverse fruits of two Lepidium species differ in allocation of glucosinolates into immature and mature seed and pericarp

PLoS ONE ◽  
2020 ◽  
Vol 15 (8) ◽  
pp. e0227528
Author(s):  
Said Mohammed ◽  
Samik Bhattacharya ◽  
Matthias Alexander Gesing ◽  
Katharina Klupsch ◽  
Günter Theißen ◽  
...  
Keyword(s):  
Mature Seed

High Levels of Sugars and Fructan in Mature Seed of Sweet Wheat Lacking GBSSI and SSIIa Enzymes

2011 ◽  
Vol 59 (9) ◽  
pp. 4794-4800 ◽  
Author(s):  
Tomoya Shimbata ◽  
Takayuki Inokuma ◽  
Ai Sunohara ◽  
Patricia Vrinten ◽  
Mika Saito ◽  
...  
Keyword(s):  
Mature Seed

Spatial and temporal regulation of a soybean (Glycine max) lectin promoter in transgenic cotton (Gossypium hirsutum)

2002 ◽  
Vol 29 (7) ◽  
pp. 835 ◽  
Author(s):  
Belinda J. Townsend ◽  
Danny J. Llewellyn
Keyword(s):  
Glycine Max ◽  
Gossypium Hirsutum ◽  
Embryo Development ◽  
Promoter Activity ◽  
Gene Promoter ◽  
Transgenic Cotton ◽  
Gus Activity ◽  
Mature Seed ◽  
Lectin Gene ◽  
Lectin Promoter

The activity of a soybean (Glycine max L. Merrill) lectin gene promoter was investigated in transgenic cotton plants (Gossypium hirsutum L.) with the view to using this promoter for the seed-specific alteration of gossypol, a secondary metabolite in cotton that has adverse effects on the nutritional value of cottonseed products like oil and protein-rich meal. Agrobacterium-mediated transformation generated stable transformants containing a construct with the lectin promoter fused to the β-glucuronidase reporter gene (pLeGUS). Fluorometric GUS assays and northern hybridization detected strong promoter activity during embryo development. GUS activity in developing embryos was detected as early as 10 d post-anthesis (dpa), peaking late in embryo maturation. Enzyme activity persisted in imbibed mature seed, and negligible activity remained detectable in the roots and cotyledons of 7-d-old seedlings. No GUS activity was detected in leaves and squares of mature plants. GUS transcripts increased during embryo development to peak about 35 dpa, declining to a low level in imbibed mature seed. No transcripts were detected in roots, cotyledons, leaves or squares. Histochemical GUS activity staining indicated promoter activity in all cells of the cotyledons, including the flattened cells of the gossypol glands, the presumed site of synthesis of gossypol. This study concluded that the soybean lectin gene promoter is a useful tool for the seed-specific expression of transgenes in cotton.

scholarly journals Re-evaluation of Mature Seed-derived Callusing and Regeneration Potential of Nine Orchardgrass (Dactylis golomerata L.) Cultivars

1970 ◽  
Vol 17 (2) ◽  
pp. 193-207 ◽  
Author(s):  
Sang-Hoon Lee ◽  
Nagib Ahsan ◽  
Ki-Won Lee ◽  
Dong-Gi Lee ◽  
Iftekhar Alam ◽  
...  
Keyword(s):  
Key Words ◽  
Callus Induction ◽  
Plant Tissue ◽  
Genotypic Variation ◽  
Carbon Sources ◽  
Mature Seed ◽  
Regeneration Potential ◽  
Efficient Regeneration ◽  
N6 Medium ◽  
Mature Seeds

A suitable callus induction and efficient regeneration protocol for orchardgrass (Dactylis golomerata L.) was developed. It consisted of 3 mg/l 2,4-D + 0.1 mg/l BA + 1 g/l CH + 300 mg/l L-proline + 40 mg/l L-cysteine + 30 g/l sucrose in MS showed the highest percentage of callus induction. Maltose exhibited better in regeneration than other types of carbon sources. Highest (71%) regeneration was obtained from N6 medium containing 1 mg/l 2,4-D + 3 mg/l BA + 1 g/l CH + 300 mg/l L-proline + 40 mg/l L-cysteine + 30 g/l maltose. Among the nine cultivars of orchardgrass (Dactylis golomerata L.), genotypic variation was observed in both callus induction and regeneration. Overall callus induction and regeneration rates were 23 - 73 and 17 - 71%, respectively.  Key words: Dactylis golomerata, Orchardgrass, Mature seeds, Additives, Regeneration, Maltose. D.O.I. 10.3329/ptcb.v17i2.3240 Plant Tissue Cult. & Biotech. 17(2): 193-207, 2007 (December)

scholarly journals Somatic Embryogenesis in Suspension Culture of Mature Seed of Cucumis melo L.

1991 ◽  
Vol 41 (2) ◽  
pp. 273-278 ◽  
Author(s):  
Kohji KAGEYAMA ◽  
Kazunori YABE ◽  
Shigetoshi MIYAJIMA
Keyword(s):  
Somatic Embryogenesis ◽  
Suspension Culture ◽  
Cucumis Melo ◽  
Mature Seed ◽  
Cucumis Melo L

Metribuzin residues in lentil following postemergence application

1998 ◽  
Vol 78 (1) ◽  
pp. 167-169 ◽  
Author(s):  
Allan J. Cessna
Keyword(s):  
Gas Chromatography ◽  
Key Words ◽  
Lens Culinaris ◽  
Residue Analysis ◽  
Mature Seed ◽  
Western Canada

In a study involving two sites in western Canada, residues of the herbicide metribuzin in lentil were monitored by gas chromatography following postemergence application at 0.28 kg a.i. ha−1. On the day of spraying, initial residues in lentil green foliage were of the order of 1 mg kg−1. Metribuzin residues in the green foliage continually decreased with time to non-detectable levels (< 0.02 mg kg−1) at 42 d after application. No metribuzin residues were detected in the straw or mature seed. Key words: Metribuzin, 4-amino-6-(1,1-dimethylethyl)-3-(methylthio-1,2,4-triazin-5(4H)-one; lentil, Lens culinaris Medic., residues, residue analysis, gas chromatography

scholarly journals Anatomía de la semilla de Casimiroa edulis (Rutaceae), "zapote blanco", durante su desarrollo

2017 ◽  
pp. 67
Author(s):  
Hilda Araceli Zavaleta-Mancera
Keyword(s):  
Seed Development ◽  
Mature Embryo ◽  
Mature Seed ◽  
Protein Bodies ◽  
Fast Green ◽  
Oil Glands ◽  
Specific Staining ◽  
Starch Grains ◽  
Casimiroa Edulis ◽  
Mexican Species

The seed of the Mexican species, Casimiroa edulis Llave et Lexarza is valued for medicinal purposes. We have studied its anatomy in different stages of development from ovule to mature seed. We applied general staining with safranin and fast green and specific staining for the detection of starch, lipids, lignin, polyphenols, and proteins. Embryo and seed development was also evaluated on the basis of volume. The ovule is sessile, bitegmic, crassinucellate, hemianatropous, with a nucellar curvature of 30-40º and a large hilum. The seed develops a large pachychalaza that covers approximately 70% of the surface. The testa is thin, nonlignified and tanniferous. The mature embryo occupies about 90% of the whole volume of the seed and contains lysigenous oil glands, starch grains and protein bodies. The radicle is not directed exactly at the micropyle. lt is located in the dorsal micropylar third of the seed. Casimiroa edulis had been described as lacking endosperm, but in reality the mature seed has a small amount of endosperm under the pachychalaza.

Sign in / Sign up

Export Citation Format

Share Document