Physiological and biochemical changes induced by Qiangdi nano-863 biological assistant growth apparatus during rice seed priming under temperature stress

A huge amount of rice cultivation and consumption occur in Asia particularly in Pakistan and China. However, multiple abiotic stresses especially high and low-temperature proved to be a substantial threat for rice production ultimately risks for food security. To overcome various types of abiotic stress; seed priming is among the effective approaches to improve the rice seed germination and growth vigor. Therefore, the present study was planned to evaluate physiological and biochemical modifications in Chinese and Pakistani rice varieties by Qiangdi 863 biological assistant growth apparatus nano treated water (NTW), Osmopriming Calcium chloride (CaCl2), redox priming hydrogen peroxide (H2O2) and hormonal priming by Salicylic acid (SA) under temperature stress conditions. The experiment was performed with completely randomize design conditions. Five rice varieties, nomenclature as Zhongzoa 39, (Chinese rice variety) KSK 133, KS 282, Super basmati and PK 1121 aromatic (Pakistani rice variety) were sown under low temperature (LT) (17ºC), optimal temperature (OT) 27ºC and high temperature (HT) 37ºC conditions. The present study indicated that nanopriming were the most effective treatments increased Germination Energy Percentage (GEP) (96.1, 100, 100%), Speed of Germination (SG) (27.2, 35.45, 37.1), Final Germination Percentage (FGP) (98.2, 99.1, 99.4%), Seedling Dry Weight Biomass (DWB) (0.1, 0.137, 0.14g), Total Chlorophyll Content (0.502, 13.74, 15.21), antioxidant enzymes Superoxide Dismutase (SOD)(3145, 2559, 3345 µg-1FWh-1), Catalase (CAT) (300, 366, 3243 µg-1FWh-1) and decreased Malondialdehyde (MDA) (6.5, 12.2, 6.5 µmol g-1 FW) for Zhongzao 39 and KSK 133 rice varieties under low (LT+NTW), optimal temperature (OP+NTW) and high temperature (HT+NTW) stress., Therefore, nano-priming is recommended to cope with the high and low-temperature stress conditions along with improved productivity of rice.

Characterization of Multiple Grain Rice (Oryza sativa L.) Biramsundari from Bangladesh

Biramsundari is a rice germplasm from Bangladesh showing one to four grain in a single seed. Comparative study of morphological traits revealed that BS is a taller rice variety compared to modern rice varieties with longer and wider flag leaves, longer panicle length and higher thousand seed weight (TSW) than transplanted aman rice variety BRRI dhan 49. Flower morphological analysis unveil that multiple grains of Biramsundari are originating from multiple number of carpels in each floret. About 40.1% flower contains three carpels. Fluorescent microscopic study also confirms the zygotic origin of multiple grain formation in Biramsundari. Molecular characterisation of Biramsundari was performed by using TeaCpSSR27 and TeaCpSSR28 chloroplast microsatellite markers. The results of this investigation reveal that atpF and rsp14-psaB intergenic spacer regions of Biramsundari have variation compared to sequences of with O. sativa ssp. indica, O. sativa ssp. japonica and O. rufipogon. Plant Tissue Cult. & Biotech. 31(2): 115-122, 2021 (December)

Genotype and phenotype variability of BC1F1 progenies from backcross of C3 and Koshihikari

Improvement of local rice variety could be conducted by crossing it with superior varieties. C3 is improved line of Acehnese rice ‘Cantik Manis’ by crossing it with the introduced Chinese rice variety Yin Zhan to make their progenies have shorter lifespan and good productivity. Although C3 line has been improved, this variety still has lifespan for about ± 135 days. Koshihikari is Japanese rice variety with its lifespan about 80-90 days. It is though that Koshihikari has various semidwarf gene such as sd-1 or other semidwarf genes. The strategy that can be used to decrease harvesting period of C3 is by crossing this variety with Koshihikari to insert semi-dwarf gene such as sd-1. The research was conducted to analyse the existing of sd-1 gen and phenotypic performance of their progenies specially in BC1F1. The seed of BC1F1 were found by crossing 2 prospective F1 plants (T8 and T10) with C3 parent. Molecular analysis showed that the whole progenies BC1F1-T8 inherit sd-1 gene (100%). Phenotypic observation showed the whole progenies had similar architecture as their parents with average heights at 45 DAP ranged from 74-91 cm, and number of tiller range from 3-9 tillers. Whereas in BC1F1-T10 progenies, there were 11 plant from 12 plants inherit sd-1 gene (91.7%). These progenies had average heights at 45 DAP ranged from 75-88 cm, and number of tiller range from 6-13 tillers.

Effect of Soil Application of Zinc on Growth, Yield and Zinc Concentration in Rice Varieties

Zinc (Zn) deficiency is widespread nutrient disorder in lowland rice growing areas in Asia, especially in Bangladesh. Intensive cropping with modern varieties causes depletion of inherent nutrient reserves in soils. The application of Zn fertilizers results in higher crop productivity and increases Zn concentration in crops. A field experiment was conducted to evaluate the effect of Zn application on growth, yield, and grain-Zn concentration in eight varieties of rice. The experiment was laid out in a split plot design with a distribution of Zn rates (0 kg ha-1 and 3 kg ha-1 from ZnO) to the main plots and rice varieties (BRRI dhan49, BRRI dhan52, BRRI dhan56, BRRI dhan57, Kalizira, Biroin, Gainja and Khirshapath) to the sub-plots. Zinc application improved effective tillers hill-1, grains panicle-1 and 1000-grain weight which impacted the grain yield of rice. Among the eight rice varieties, a significant increase of grain yield was recorded in BRRI dhan49, BRRI dhan52, BRRI dhan56 and BRRI dhan57 due to application of Zn. Zinc concentration of grain significantly increased in all rice varieties except Biroin. The highest grain-Zn concentration (19.1 mg kg-1) was noted in BRRI dhan57 with 3 kg ha-1 Zn and the lowest value (11.3 mg kg-1) was observed in BRRI dhan52 without Zn application. The highest percent increase of grain Zn concentration over control was obtained in high yielding rice variety BRRI dhan49 and the lowest Zn concentration was found in local rice variety Biroin.

Profitability and productivity of drought tolerant Aus rice variety Binadhan-19 in some areas of Bangladesh

The present study was conducted in five districts namely Mymensingh, Ranpur, Pabna, Rajshahi and Chapainwabganj in Bangladesh. The objectives of the study were i) to identify the socio-economic characteristics of Aus rice growers; ii) to estimate profitability and productivity of Binadhan-19 in the study areas; and iii) to find out the major preferences and constraints for the variety cultivation. A total of 200 farmers were randomly selected (40 from each location) to collect the data with a pre-designed questionnaire. The distribution of the farmers by age showed that the mean age for Binadhan-19 cultivated farmers was 43 years. In the study areas among the farmer 86% was educated. Farmers average experience was 20 year and income were Tk. 235066 (USD 2611.84) per year. Per hectare average yield of rice was 1.37 ton. It was estimated that, to produce one kilogram of rice, total cost incurred was Tk. 14 where per kg average selling price of rice was Tk. 17. The average gross return and gross margin of rice cultivation were found Tk. 90679 (USD 1007.54)/ha and Tk. 51290 (USD 559.88)/ha, respectively. Per hectare average net return was Tk. 2459 (USD 27.32) which was found to be highest in Chapainwabganj Tk. 29739 (USD 330.43) and lowest in Rangpur Tk. 12692 (USD 141.02) district. BCR on total cost basis was found 1.37. The highest preference was for neat rice 98% and the highest constrain said by the farmer was crop destroy by animal and bird of paddy for early ripening in Binadhan-19 cultivation.

Acaulospora as the Dominant Arbuscular Mycorrhizal Fungi in Organic Lowland Rice Paddies Improves Phosphorus Availability in Soils

Flooding in rainfed lowlands greatly impairs the mutualistic relationship between indigenous arbuscular mycorrhizal fungi (AMF) and rice. In flooded soils, root colonization by AMF is arrested, but some AMF genera, defined as the core AMF, remain present. However, the core AMF in rainfed lowlands and their symbiotic roles remain unknown. Here, we showed that Acaulospora fungi were the core AMF in rice seedling roots of the Sangyod Muang Phatthalung (SMP) landrace rice variety grown in non-flooded and flooded paddy soils. Subsequently, indigenous Acaulospora spores were propagated by trap cultures using maize as the host plants. Therefore, to clarify the roles of cultured Acaulospora spores in a symbiotic partnership, the model japonica rice variety Nipponbare was grown in sterile soil inoculated with Acaulospora spores, and recolonized with a native microbial filtrate from the organic rice paddy soil. Our data demonstrated that the inoculation of Acaulospora spores in well-drained soil under a nutrient-sufficient condition for six weeks enabled 70 percent of the rice roots to be colonized by the fungi, leading to higher phosphate (Pi) accumulation in the mycorrhizal roots. Unexpectedly, the growth of rice seedlings was significantly suppressed by inoculation while photosynthetic parameters such as fractions of incoming light energy and relative chlorophyll content were unaltered. In the soil, the Acaulospora fungi increased soil phosphorus (P) availability by enhancing the secretion of acid phosphatase in the mycorrhizal roots. The findings of this work elucidate the symbiotic roles of the dominant Acaulospora fungi from lowland rice paddies.

Effect of soil and crop management practices on sodicity stress alleviation and rice productivity under water scarce condition

The prominent issue faced by the farmers of Cauvery Delta regionin Tamil Nadu particularly Tiruchirapalli District is the lack of timely release of water for rice nursery preparation and transplanting. Hence wet seeding of rice is recommended for timely cultivation. On the other hand, sodic nature of the soil warrants rice cultivation only. Therefore, the present investigation was carried out to study the different soil and crop management practices on alleviating sodicity stress and improving rice (variety ADT 3) productivity under water-scarce conditions. The experiment was laid in a split plot design with six mainplots,including rice wet seeding, daincha (Sesbania aculeata) application as green manure, anti-oxidant microbial consortia (AOMC) spray and four sub-plots with graded levels of NPK based on soil test values with three replication. Results showed that the daincha incorporation @ 6.25 t/ha followed by rice wet seeding + AOMC spray @1.5 % with 125 % soil test based NPK had significantly increased thechlorophyll content, SPAD values, proline content and grain and straw yields which remained on par with daincha incorporation @ 6.25 t/ha followed by rice wet seeding + AOMC spray @1.5 % with 100 % soil test based NPK.Significantly lower ESP at 5% level and higher phosphatase activity in soil was also recorded by daincha incorporation @ 6.25 t/ha followed by rice wet seeding + AOMC spray @1.5 % with 125 % and 100 % soil test based NPK. Gross return, net return and B:C ratio were also higher in the plot, which received daincha incorporation @6.25 t/ha followed by rice wet seeding + AOMC spray @1.5 % with 100 % soil test based NPK. The present study reveals that the inclusion of ectophytic microbial population spray in rice plants and the management practices helps the crop to tolerate the sodicity stress under water-scarce condition by maintaining required physiological functions like proline synthesis and enzyme activities etc which need to be further explored at the genotypic level.

Trace Metal Accumulation in Rice Variety Kainat Irrigated with Canal Water

Due to the rapid increase in industrial and urban areas, environmental pollution is increasing worldwide, causing unwanted changes in the air, water, and soil at biological, physical, and chemical levels, ultimately causing negative effects for living things. This work was performed in Jhang, Punjab, Pakistan, and examined and measured heavy metal levels in various plant parts of the rice (Oryza sativa) variety Kainat (roots, shoots, and grains) with results been set in relation to the soil around the root area. The samples were taken from five different sites. The mean level of trace metals (mg/kg) in grains was soil-dependent and varied from cadmium (Cd) (2.49–5.52), zinc (Zn) (5.8–10.78), copper (Cu) (4.82–7.85), cobalt (Co) (1.48–6.52), iron (Fe) (8.68–14.73), manganese (Mn) (6.87–13.93), and nickel (Ni) (2.3–8.34). Excluding Cd, the absorption of all metals under inspection was recorded within permissible limits, as recommended by the FAO and WHO. The pollution load index for Cd was highest at all sites. The enrichment coefficient of Co, Cd, and Cu were greater. The bioaccumulation factor at all studied sites was present, in order: Cu ˃ Zn ˃ Fe ˃ Mn ˃ Co ˃ Ni ˃ Cd. The translocation factor was present at five different sites: Mn ˃ Fe ˃ Cu ˃ Zn ˃ Co ˃ Cd ˃ Ni. The health risk index of all inspected metals was lower than 1 and was within safe limits. The higher pollution of Cd suggested maintenance of rice crop is recommended, decreasing health risks in humans.