scholarly journals The Central Role of cAMP in Regulating Plasmodium falciparum Merozoite Invasion of Human Erythrocytes

2014 ◽  
Vol 10 (12) ◽  
pp. e1004520 ◽  
Author(s):  
Amrita Dawn ◽  
Shailja Singh ◽  
Kunal R. More ◽  
Faiza Amber Siddiqui ◽  
Niseema Pachikara ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Human Erythrocytes ◽  
Merozoite Invasion ◽  
Falciparum Merozoite

scholarly journals Identification of Heparin Modifications and Polysaccharide Inhibitors of Plasmodium falciparum Merozoite Invasion That Have Potential for Novel Drug Development

2017 ◽  
Vol 61 (11) ◽  
Author(s):  
Michelle J. Boyle ◽  
Mark Skidmore ◽  
Benjamin Dickerman ◽  
Lynsay Cooper ◽  
Anthony Devlin ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Drug Development ◽  
Inhibitory Activity ◽  
Sulfated Polysaccharides ◽  
Merozoite Invasion ◽  
Content Type ◽  
Molecular Weights ◽  
Falciparum Merozoite ◽  
Successful Control ◽  
Novel Drug

ABSTRACT Despite recent successful control efforts, malaria remains a leading global health burden. Alarmingly, resistance to current antimalarials is increasing and the development of new drug families is needed to maintain malaria control. Current antimalarials target the intraerythrocytic developmental stage of the Plasmodium falciparum life cycle. However, the invasive extracellular parasite form, the merozoite, is also an attractive target for drug development. We have previously demonstrated that heparin-like molecules, including those with low molecular weights and low anticoagulant activities, are potent and specific inhibitors of merozoite invasion and blood-stage replication. Here we tested a large panel of heparin-like molecules and sulfated polysaccharides together with various modified chemical forms for their inhibitory activity against P. falciparum merozoite invasion. We identified chemical modifications that improve inhibitory activity and identified several additional sulfated polysaccharides with strong inhibitory activity. These studies have important implications for the further development of heparin-like molecules as antimalarial drugs and for understanding merozoite invasion.

Protein–protein interaction studies reveal the Plasmodium falciparum merozoite surface protein-1 region involved in a complex formation that binds to human erythrocytes

2018 ◽  
Vol 475 (6) ◽  
pp. 1197-1209 ◽  
Author(s):  
Gourab Paul ◽  
Arunaditya Deshmukh ◽  
Bishwanath Kumar Chourasia ◽  
Md Kalamuddin ◽  
Ashutosh Panda ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Recombinant Proteins ◽  
Surface Protein ◽  
Merozoite Surface Protein ◽  
Human Erythrocytes ◽  
Proteolytic Processing ◽  
Protein Protein Interaction ◽  
Falciparum Merozoite ◽  
Vaccine Candidate Antigen ◽  
Serological Studies

Plasmodium falciparum merozoite surface protein (PfMSP) 1 has been studied extensively as a vaccine candidate antigen. PfMSP-1 undergoes proteolytic processing into four major products, such as p83, p30, p38, and p42, that are associated in the form of non-covalent complex(s) with other MSPs. To delineate MSP1 regions involved in the interaction with other MSPs, here we expressed recombinant proteins (PfMSP-165) encompassing part of p38 and p42 regions and PfMSP-119. PfMSP-165 interacted strongly with PfMSP-3, PfMSP-6, PfMSP-7, and PfMSP-9, whereas PfMSP-119 did not interact with any of these proteins. Since MSP-1 complex binds human erythrocytes, we examined the ability of these proteins to bind human erythrocyte. Among the proteins of MSP-1 complex, PfMSP-6 and PfMSP-9 bound to human erythrocytes. Serological studies showed that PfMSP-165 was frequently recognized by sera from malaria endemic regions, whereas this was not the case for PfMSP-119. In contrast, antibodies against PfMSP-119 showed much higher inhibition of merozoite invasion compared with antibodies against the larger PfMSP-165 fragment. Importantly, anti-PfMSP-119 antibodies recognized both recombinant proteins, PfMSP-119 and PfMSP-165; however, anti-PfMSP-165 antibody failed to recognize the PfMSP-119 protein. Taken together, these results demonstrate that PfMSP-1 sequences upstream of the 19 kDa C-terminal region are involved in molecular interactions with other MSPs, and these sequences may probably serve as a smoke screen to evade antibody response to the membrane-bound C-terminal 19 kDa region.

scholarly journals Stepwise dissection of Plasmodium falciparum merozoite invasion of the human erythrocyte

2010 ◽  
Vol 9 (S2) ◽  
Author(s):  
David Riglar ◽  
Dave Richard ◽  
Michelle Boyle ◽  
Danny Wilson ◽  
Fiona Angrisano ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Human Erythrocyte ◽  
Merozoite Invasion ◽  
Falciparum Merozoite

scholarly journals Involvement of Pf155/RESA and cross-reactive antigens in Plasmodium falciparum merozoite invasion in vitro.

1992 ◽  
Vol 60 (2) ◽  
pp. 443-449 ◽  
Author(s):  
B Wåhlin ◽  
A Sjölander ◽  
N Ahlborg ◽  
R Udomsangpetch ◽  
A Scherf ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Merozoite Invasion ◽  
Falciparum Merozoite

scholarly journals Antibodies against a short region of PfRipr inhibit Plasmodium falciparum merozoite invasion and PfRipr interaction with Rh5 and SEMA7A

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Hikaru Nagaoka ◽  
Bernard N. Kanoi ◽  
Edward H. Ntege ◽  
Masamitsu Aoki ◽  
Akihisa Fukushima ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Merozoite Invasion ◽  
Short Region ◽  
Falciparum Merozoite

Effect of Different Fractions of Heparin on Plasmodium falciparum Merozoite Invasion of Red Blood Cells in Vitro

1992 ◽  
Vol 46 (5) ◽  
pp. 589-594 ◽  
Author(s):  
Asli Kulane ◽  
Birgitta Wahlin ◽  
Peter Perlmann ◽  
Hans-Peter Ekre ◽  
Lars Rombo ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Merozoite Invasion ◽  
Falciparum Merozoite

Two MSA 2 peptides that bind to human red blood cells are relevant to Plasmodium falciparum merozoite invasion

2000 ◽  
Vol 55 (3) ◽  
pp. 216-223 ◽  
Author(s):  
M. Ocampo ◽  
M. Urquiza ◽  
F. Guzmán ◽  
L.E. Rodriguez ◽  
J. Suarez ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Red Blood Cells ◽  
Blood Cells ◽  
Merozoite Invasion ◽  
Human Red Blood Cells ◽  
Falciparum Merozoite
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