scholarly journals An In Vitro Model of Latency and Reactivation of Varicella Zoster Virus in Human Stem Cell-Derived Neurons

2015 ◽  
Vol 11 (6) ◽  
pp. e1004885 ◽  
Author(s):  
Amos Markus ◽  
Ilana Lebenthal-Loinger ◽  
In Hong Yang ◽  
Paul R. Kinchington ◽  
Ronald S. Goldstein
Keyword(s):  
Stem Cell ◽  
Varicella Zoster Virus ◽  
In Vitro Model ◽  
Human Stem Cell ◽  
Varicella Zoster ◽  
Vitro Model

Human Stem Cell-Derived In Vitro Model

2011 ◽  
Vol 31 (9) ◽  
pp. 26-27
Author(s):  
Lucas Chase,
Keyword(s):  
Stem Cell ◽  
In Vitro Model ◽  
Human Stem Cell ◽  
Vitro Model

P212 STEM CELL-DERIVED HEPATOCYTES AS A NOVEL IN VITRO MODEL TO STUDY HEPATOTROPIC VIRUSES

2014 ◽  
Vol 60 (1) ◽  
pp. S136
Author(s):  
N. Helsen ◽  
J. Paeshuyse ◽  
Y. Debing ◽  
K. Dallmeier ◽  
J. Neyts ◽  
...  
Keyword(s):  
Stem Cell ◽  
In Vitro Model ◽  
Vitro Model

scholarly journals Microparticle-mediated VZV propagation and endothelial activation

Neurology ◽  
2019 ◽  
Vol 94 (5) ◽  
pp. e474-e480 ◽  
Author(s):  
Despina Eleftheriou ◽  
Elena Moraitis ◽  
Ying Hong ◽  
Mark Turmaine ◽  
Cristina Venturini ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Vascular Remodeling ◽  
In Vitro Model ◽  
Cerebral Arteries ◽  
Virus Transmission ◽  
Endothelial Activation ◽  
Arterial Ischemic Stroke ◽  
Varicella Zoster ◽  
Vitro Model

ObjectiveVaricella zoster virus (VZV) can spread anterogradely and infect cerebral arteries causing VZV vasculopathy and arterial ischemic stroke. In this study, we tested the hypothesis that virus-infected cerebrovascular fibroblasts undergo phenotypic changes that promote vascular remodeling and facilitate virus transmission in an in vitro model of VZV vasculopathy. The aims of this project were therefore to examine the changes that virus-infected human brain adventitial vascular fibroblasts (HBVAFs) undergo in an in vitro model of VZV vasculopathy and to identify disease biomarkers relating to VZV-related vasculopathy.MethodsHBVAFs were infected with VZV, and their ability to migrate, proliferate, transdifferentiate, and interact with endothelial cells was studied with flow cytometry. Microparticles (MPs) released from these cells were isolated and imaged with transmission electron microscopy, and their protein content was analyzed with mass spectrometry. Circulating MP profiles were also studied in children with VZV and non-VZV vasculopathy and compared with controls.ResultsVZV-infected HBVAFs transdifferentiated into myofibroblasts with enhanced proliferative and migratory capacity. Interaction of VZV-infected HBVAFs with endothelial cells resulted in endothelial dysfunction. These effects were, in part, mediated by the release of MPs from VZV-infected HBVAFs. These MPs contained VZV virions that could transmit VZV to neighboring cells, highlighting a novel model of VZV cell-to-cell viral dissemination. MPs positive for VZV were significantly higher in children with VZV-related vasculopathy compared to children with non-VZV vasculopathy (p = 0.01) and controls (p = 0.007).ConclusionsVZV-infected HBVAFs promote vascular remodeling and facilitate virus transmission. These effects were mediated by the release of apoptotic MPs that could transmit VZV infection to neighboring cells through a Trojan horse means of productive viral infection. VZV+ MPs may represent a disease biomarker worthy of further study.

scholarly journals Human induced-pluripotent stem cell-derived hepatocyte-like cells as an in vitro model of human hepatitis B virus infection

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Fuminori Sakurai ◽  
Seiji Mitani ◽  
Tatsuro Yamamoto ◽  
Kazuo Takayama ◽  
Masashi Tachibana ◽  
...  
Keyword(s):  
Stem Cell ◽  
Hepatitis B Virus ◽  
Pluripotent Stem Cell ◽  
In Vitro Model ◽  
Induced Pluripotent Stem Cell ◽  
Hepatitis B Virus Infection ◽  
B Virus ◽  
Vitro Model ◽  
Induced Pluripotent
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