Polymerase Chain Reaction of Pleural Biopsy Is a Rapid and Sensitive Method for the Diagnosis of Tuberculous Pleural Effusion

CHEST Journal ◽  
2003 ◽  
Vol 124 (6) ◽  
pp. 2105-2111 ◽  
Author(s):  
Nadia A. Hasaneen ◽  
Maysaa E. Zaki ◽  
Hala M. Shalaby ◽  
Ahmad S. El-Morsi
Keyword(s):  
Polymerase Chain Reaction ◽  
Pleural Effusion ◽  
Chain Reaction ◽  
Pleural Biopsy ◽  
Tuberculous Pleural Effusion ◽  
Polymerase Chain ◽  
Sensitive Method

scholarly journals New sensitive method for the detection of the A3243G mutation of human mitochondrial deoxyribonucleic acid in diabetes mellitus patients by ligation-mediated polymerase chain reaction

1998 ◽  
Vol 44 (10) ◽  
pp. 2088-2093 ◽  
Author(s):  
Michiyo Urata ◽  
Machiko Wakiyama ◽  
Masanori Iwase ◽  
Makoto Yoneda ◽  
Sachiko Kinoshita ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Polymerase Chain Reaction ◽  
Conventional Method ◽  
Peripheral Blood ◽  
Blood Cells ◽  
Nucleotide Position ◽  
Peripheral Blood Cells ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Sensitive Method

Abstract An adenine-to-guanine mutation at nucleotide position (np) 3243 in the mitochondrial tRNALeu(UUR) gene is closely associated with various clinical phenotypes of diabetes mellitus. Because the mutation creates a new restriction site for the restriction enzyme ApaI, the mutation is usually detected and quantified by ApaI cleavage of the PCR products including np 3243. The sensitivity of the conventional method is, however, 5–10% heteroplasmy. The percentage of heteroplasmy of the mutation is usually highest in the affected tissues and is much lower in peripheral blood cells, which are used most frequently for the analysis. The sensitivity of the conventional method, however, is not sufficient to detect the mutation from peripheral blood cells. Utilizing ligation-mediated polymerase chain reaction, we have developed a feasible and sensitive method to detect 0.01% heteroplasmy of the 3243 mutation in peripheral leukocytes.

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