Substrate Selectivity of Gluconobacter oxydans for Production of 2,5-Diketo-D-Gluconic Acid and Synthesis of 2-Keto-L-Gulonic Acid in a Multienzyme System

2001 ◽  
Vol 94 (3) ◽  
pp. 213-224 ◽  
Author(s):  
Aiguo Ji ◽  
Peiji Gao
Keyword(s):  
Gluconic Acid ◽  
Gluconobacter Oxydans ◽  
Substrate Selectivity ◽  
Multienzyme System

Directing cell catalysis of glucose to 2-keto-d-gluconic acid using Gluconobacter oxydans NL71

2020 ◽  
Vol 94 ◽  
pp. 365-369
Author(s):  
Xuelian Zhou ◽  
Yi Shen ◽  
Yong Xu ◽  
Venkatesh Balan
Keyword(s):  
Gluconic Acid ◽  
Gluconobacter Oxydans

scholarly journals Overexpression of membrane-bound gluconate-2-dehydrogenase to enhance the production of 2-keto-d-gluconic acid by Gluconobacter oxydans

2016 ◽  
Vol 15 (1) ◽  
Author(s):  
Kefei Li ◽  
Xinlei Mao ◽  
Liu Liu ◽  
Jinping Lin ◽  
Ming Sun ◽  
...  
Keyword(s):  
Gluconic Acid ◽  
Gluconobacter Oxydans ◽  
Membrane Bound

scholarly journals Screening of Thermotolerant Gluconobacter Strains for Production of 5-Keto-d-Gluconic Acid and Disruption of Flavin Adenine Dinucleotide-Containing d-Gluconate Dehydrogenase

2009 ◽  
Vol 75 (13) ◽  
pp. 4240-4247 ◽  
Author(s):  
Ittipon Saichana ◽  
Duangtip Moonmangmee ◽  
Osao Adachi ◽  
Kazunobu Matsushita ◽  
Hirohide Toyama
Keyword(s):  
Gluconic Acid ◽  
Flavin Adenine Dinucleotide ◽  
Gluconobacter Oxydans ◽  
Gene Cassette ◽  
Kanamycin Resistance ◽  
Major Product ◽  
Inverse Pcr ◽  
Glycerol Dehydrogenase ◽  
Mutant Strains

ABSTRACT We isolated thermotolerant Gluconobacter strains that are able to produce 5-keto-d-gluconic acid (5KGA) at 37°C, a temperature at which regular mesophilic 5KGA-producing strains showed much less growth and 5KGA production. The thermotolerant strains produced 2KGA as the major product at both 30 and 37°C. The amount of ketogluconates produced at 37°C was slightly less than the amount produced at 30°C. To improve the yield of 5KGA in these strains, we disrupted flavin adenine dinucleotide-gluconate dehydrogenase (FAD-GADH), which is responsible for 2KGA production. Genes for FAD-GADH were cloned by using inverse PCR and an in vitro cloning strategy. The sequences obtained for three thermotolerant strains were identical and showed high levels of identity to the FAD-GADH sequence reported for the genome of Gluconobacter oxydans 621 H. A kanamycin resistance gene cassette was used to disrupt the FAD-GADH genes in the thermotolerant strains. The mutant strains produced 5KGA exclusively, and the final yields were over 90% at 30°C and 50% at 37°C. We found that the activity of pyrroloquinoline quinone (PQQ)-dependent glycerol dehydrogenase, which is responsible for 5KGA production, increased in response to addition of PQQ and CaCl2 in vitro when cells were grown at 37°C. Addition of 5 mM CaCl2 to the culture medium of the mutant strains increased 5KGA production to the point where over 90% of the initial substrate was converted. The thermotolerant Gluconobacter strains that we isolated in this study provide a promising new option for industrial 5KGA production.

High-yield 5-keto-d-gluconic acid formation is mediated by soluble and membrane-bound gluconate-5-dehydrogenases of Gluconobacter oxydans

2006 ◽  
Vol 73 (2) ◽  
pp. 443-451 ◽  
Author(s):  
Marcel Merfort ◽  
Ute Herrmann ◽  
Stephanie Bringer-Meyer ◽  
Hermann Sahm
Keyword(s):  
Gluconic Acid ◽  
Gluconobacter Oxydans ◽  
Acid Formation ◽  
High Yield ◽  
Membrane Bound

Biotransformation of glucose to 5-keto-d-gluconic acid by recombinant Gluconobacter oxydans DSM�2343

2004 ◽  
Vol 64 (1) ◽  
pp. 86-90 ◽  
Author(s):  
U. Herrmann ◽  
M. Merfort ◽  
M. Jeude ◽  
S. Bringer-Meyer ◽  
H. Sahm
Keyword(s):  
Gluconic Acid ◽  
Gluconobacter Oxydans
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