Summary
New Trichoderma isolates were prepared from soils collected in the areas of the world where the
dry rot fungus Serpula lacrymans is indigenous. Agar-screening tests were carried out to identify
the antagonistic effects of isolates and determine if their mode of action was geared towards production
of volatiles. Overall, Trichoderma isolates from Northern California, USA, were found to
be extremely effective against S. lacrymans (Schumacher ex Fr. Gray.) and some produced a killing
effect in the agar systems. In addition, these isolates inhibited S. lacrymans growth by volatile production
even in the absence of direct contact between the Trichoderma isolate and S. lacrymans,
whereas a standard laboratory culture of Trichoderma harzianum T25 was found to be much less
effective. In two different soil and wood microcosms the USA isolates were also able to stop the
growth of S. lacrymans and produced an apparent killing effect.
COMPARING PLEURAL FLUID CULTURE YIELD IN BEDSIDE INOCULATION AND STANDARD LABORATORY CULTURE