Molecular genetic characterisation of stripe rust resistance genes from Vavilov’s wheat collection

Stripe rust, caused by Puccinia striiormis Westend f. sp. tritici, is one of the most important foliar diseases of wheat ( Triticum aestivum L.) worldwide. Stripe rust resistance genes Yr27, Yr31, YrSp, YrV23, and YrCN19 on chromosome 2BS confer resistance to some or all Chinese P. striiormis f. sp. tritici races CYR31, CYR32, SY11-4, and SY11-14 in the greenhouse. To screen microsatellite (SSR) markers linked with YrCN19, F1, F2, and F3 populations derived from cross Ch377/CN19 were screened with race CYR32 and 35 SSR primer pairs. Linkage analysis indicated that the single dominant gene YrCN19 in cultivar CN19 was linked with SSR markers Xgwm410, Xgwm374, Xwmc477, and Xgwm382 on chromosome 2BS with genetic distances of 0.3, 7.9, 12.3, and 21.2 cM, respectively. Crosses of CN19 with wheat lines carrying other genes on chromosome 2B showed that all were located at different loci. YrCN19 is thus different from the other reported Yr genes in chromosomal location and resistance response and was therefore named Yr41. Prospects and strategies of using Yr41 and other Yr genes in wheat improvement for stripe rust resistance are discussed.

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Molecular Marker Based Design for Breeding Wheat Lines with Multiple Resistance and Superior Quality

Plant Disease ◽

10.1094/pdis-02-20-0420-re ◽

2020 ◽

Vol 104 (10) ◽

pp. 2658-2664

Author(s):

Tao Liu ◽

George Fedak ◽

Lianquan Zhang ◽

Rangrang Zhou ◽

Dawn Chi ◽

...

Keyword(s):

Stripe Rust ◽

Resistance Genes ◽

High Resistance ◽

Stem Rust ◽

Rust Resistance ◽

Stripe Rust Resistance ◽

Multiple Resistance ◽

Wheat Stem Rust ◽

Wheat Production ◽

Rust Resistance Genes

There has not been a major wheat stem rust epidemic worldwide since the 1970s, but the emergence of race TTKSK of Puccinia graminis f. sp. tritici in 1998 presented a great threat to the world wheat production. Single disease-resistance genes are usually effective for only several years before the pathogen changes genetically to overcome the resistance. Stripe rust caused by Puccinia striiformis f. sp. tritici (Pst) is one of the most common and persistent wheat diseases worldwide. The development of varieties with multiple resistance is the most economical and effective strategy for preventing stripe rust and stem rust, the two main rust diseases constraining wheat production. Plateau 448 has been widely used in the spring wheat growing region in northwest China, but it has become susceptible to stripe rust and is susceptible to TTKSK. To produce more durable resistance to race TTKSK as well as to stripe rust, four stem rust resistance genes (Sr33, Sr36, Sr-Cad, and Sr43) and three stripe rust resistance genes (Yr5, Yr18, and Yr26) were simultaneously introgressed into Plateau 448 to improve its stem rust (Ug99) and stripe rust resistance using a marker-assisted backcrossing strategy combined with phenotypic selection. We obtained 131 BC1F5 lines that pyramided two to four Ug99 resistance genes and one to two Pst resistance genes simultaneously. Thirteen of these lines were selected for their TTKSK resistance, and all of them exhibited near immunity or high resistance to TTKSK. Among the 131 pyramided lines, 95 showed high resistance to mixed Pst races. Nine lines exhibited not only high resistance to TTKSK and Pst but also better agronomic traits and high-molecular-weight glutenin subunit compositions than Plateau 448.

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Development of a PCR Assay and Marker-Assisted Transfer of Leaf Rust and Stripe Rust Resistance Genes Lr57 and Yr40 into Hard Red Winter Wheats

Crop Science ◽

10.2135/cropsci2008.03.0143 ◽

2009 ◽

Vol 49 (1) ◽

pp. 120-126 ◽

Cited By ~ 11

Author(s):

Vasu Kuraparthy ◽

Shilpa Sood ◽

Deven R. See ◽

Bikram S. Gill

Keyword(s):

Leaf Rust ◽

Stripe Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Stripe Rust Resistance ◽

Pcr Assay ◽

Rust Resistance Genes

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Identification of Stripe Rust Resistance Genes in Wheat Genotypes Used to Differentiate North American Races ofPuccinia striiformis

Phytopathology ◽

10.1094/phyto-82-1428 ◽

1992 ◽

Vol 82 (12) ◽

pp. 1428 ◽

Cited By ~ 56

Author(s):

Xianming Chen

Keyword(s):

Stripe Rust ◽

Resistance Genes ◽

North American ◽

Rust Resistance ◽

Stripe Rust Resistance ◽

Wheat Genotypes ◽

Rust Resistance Genes

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Mapping stripe rust resistance genes by BSR-Seq: YrMM58 and YrHY1 on chromosome 2AS in Chinese wheat lines Mengmai 58 and Huaiyang 1 are Yr17

The Crop Journal ◽

10.1016/j.cj.2017.03.002 ◽

2018 ◽

Vol 6 (1) ◽

pp. 91-98 ◽

Cited By ~ 13

Author(s):

Yong Wang ◽

Huaizhi Zhang ◽

Jingzhong Xie ◽

Bingmin Guo ◽

Yongxing Chen ◽

...

Keyword(s):

Stripe Rust ◽

Resistance Genes ◽

Rust Resistance ◽

Stripe Rust Resistance ◽

Wheat Lines ◽

Chinese Wheat ◽

Rust Resistance Genes

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Molecular genetic description of the cryptic wheat–Aegilops geniculata introgression carrying rust resistance genes Lr57 and Yr40 using wheat ESTs and synteny with rice

Genome ◽

10.1139/g09-076 ◽

2009 ◽

Vol 52 (12) ◽

pp. 1025-1036 ◽

Cited By ~ 11

Author(s):

Vasu Kuraparthy ◽

Shilpa Sood ◽

Bikram S. Gill

Keyword(s):

Resistance Genes ◽

Rust Resistance ◽

Genomic Sequence ◽

Molecular Genetic ◽

Rice Chromosome ◽

Stripe Rust Resistance ◽

Comparative Genomic ◽

Aegilops Geniculata ◽

Rice Genomic Sequence ◽

Rust Resistance Genes

The cryptic wheat–alien translocation T5DL·5DS-5MgS(0.95), with leaf rust and stripe rust resistance genes Lr57 and Yr40 transferred from Aegilops geniculata (UgMg) into common wheat, was further analyzed. Molecular genetic analysis using physically mapped ESTs showed that the alien segment in T5DL·5DS-5MgS(0.95) represented only a fraction of the wheat deletion bin 5DS2-0.78-1.00 and was less than 3.3 cM in length in the diploid wheat genetic map. Comparative genomic analysis indicated a high level of colinearity between the distal region of the long arm of chromosome 12 of rice and the genomic region spanning the Lr57 and Yr40 genes in wheat. The alien segment with genes Lr57 and Yr40 corresponds to fewer than four overlapping BAC or PAC clones of the syntenic rice chromosome arm 12L. The wheat–alien translocation breakpoint in T5DL·5DS-5MgS(0.95) was further localized to a single BAC clone of the syntenic rice genomic sequence. The small size of the terminal wheat–alien translocation, as established precisely with respect to Chinese Spring deletion bins and the syntenic rice genomic sequence, further confirmed the escaping nature of cryptic wheat–alien translocations in introgressive breeding. The molecular genetic resources and information developed in the present study will facilitate further fine-scale physical mapping and map-based cloning of the Lr57 and Yr40 genes.

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