scholarly journals The Mirror RNA Expression Pattern in Human Tissues

2015 ◽  



2009 ◽  
Vol 28 (1) ◽  
pp. 111 ◽  
Author(s):  
Yaqing Zhang ◽  
Qinlong Li ◽  
Feng Zhu ◽  
Jihong Cui ◽  
Kainan Li ◽  
...  


Epigenomics ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 1027-1040 ◽  
Author(s):  
Chenjing Zhang ◽  
Jingya Wang ◽  
Xiaoge Geng ◽  
Jiangfeng Tu ◽  
Huiqin Gao ◽  
...  

Aims: To profile and characterize the circular RNA (circRNA) expression pattern in poorly differentiated gastric adenocarcinoma (PDGA). Methods & materials: CircRNA expression profiles in PDGA and adjacent nontumor tissues were analyzed by microarray. Five randomly selected differentiated expressed circRNAs (DECs) were validated by real-time quantitative PCR. m6A qualification of the top 20 DECs was conducted by m6A-immunoprecipitation and real-time quantitative PCR. Results: A total of 65 DECs were found in PDGA compared with the control. Hsa_circRNA_0077837 had the largest area under the curve. Most DECs had m6A modifications, the trend of m6A modification alteration was mainly consistent with the circRNA expression level. Conclusion: Our study revealed a set of DECs and their m6A modification alterations, which may provide new insight for their potential function in PDGA.



BMC Genomics ◽  
2017 ◽  
Vol 18 (S6) ◽  
Author(s):  
Tianyi Xu ◽  
Jing Wu ◽  
Ping Han ◽  
Zhongming Zhao ◽  
Xiaofeng Song


2021 ◽  
Vol 11 (12) ◽  
pp. 1301
Author(s):  
Arrigo Fruscalzo ◽  
Luigi Viola ◽  
Maria Orsaria ◽  
Stefania Marzinotto ◽  
Michela Bulfoni ◽  
...  

Background: Recent reports indicate the potential role of the stimulated by retinoic acid 6 (STRA6) protein in developing insulin resistance. The study’s objective was to assess placental STRA6 expression and staining pattern in human pregnancy complicated by gestational diabetes mellitus (GDM). The expression pattern of further relevant genes involved in retinoid metabolism was also evaluated. Methods: A retrospective case–control study on paraffin-embedded placental tissue. Twenty-two human pregnancies affected by GDM, namely, 11 insulin-treated (iGDM) and 11 diet-controlled (dGDM), were compared with 22 normal-developed pregnancies (controls). An RT-PCR was performed in a random sample of 18 patients (six iGDM, six dGDM, and six controls) to assess RNA expression of STRA6 and further markers of retinoid metabolism. A semi-quantitative intensity evaluation at immunohistochemistry was performed for STRA6 in all 44 recruited patients. Results: STRA6 showed a decreased placental staining (9.09% vs. 68.18% positively stained samples, p < 0.05) and augmented RNA expression in dGDM patients than controls (ΔCT expression 0.473, IQR 0.403–0.566 vs. 0.149, IQR 0.092–0.276, p < 0.05). The protein staining pattern in patients affected by iGDM was comparable to controls. A reduced RNA expression of LPL, LRP1, VLDLR, and MTTP besides an augmented expression of LDLR was found in dGDM, while overexpression of LRP1 and LPL was found in iGDM patients. Unlike in the control group, significant positive correlations were found between RXRα and the proteins involved in the intracellular uptake of ROH, such as STRA6, LRP1, LRP2, and VLDLR. Conclusions: An altered placental expression and staining pattern of STRA6 were found in pregnancies complicated by GDM compared to the controls. These changes were coupled to an altered expression pattern of several other genes involved in the retinoid metabolism.







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