Localization of Nucleolar Organizer Regions (NORs) of 4 Gibbon Species in Thailand by Ag-NOR Banding Technique

This study examines for the first time of karyotypic analysis and chromosomal characteristic of nucleolar organizer regions/NORs of Epinephelus sexfasciatus. The fish samples were collected from Andaman Sea, Phuket province, southern Thailand. The chromosomes were investigated using conventional Giemsa’s staining and Ag-NORs banding techniques. Fish chromosome preparations were conducted by squash technique from kidney. The results showed that the diploid chromosome number of E. sexfasciatus was 2n=48 and the fundamental number (NF, number of chromosome arms) was 48. The type of chromosomes included 24 large telocentric and 24 medium telocentric chromosomes. After Ag-NOR banding technique, single pair of NORs was observed on the short arm of medium telocentric chromosome pair 23. The idiogram shows gradually decreasing length of the chromosomes. A size difference of the largest and the smallest chromosomes is approximately two folds. The karyotype formula could be infered as: 2n(48) = 2n(48)= Lt24+Mt24.

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A comparative chromosome study on five Minnow fishes (Cyprinidae, Cypriniformes) in Thailand

Caryologia ◽

10.36253/caryologia-1017 ◽

2021 ◽

Vol 74 (1) ◽

pp. 89-96

Author(s):

Surachest Aiumsumang ◽

Sumalee Phimphan ◽

Chatmongkon Suwannapoom ◽

Patcharaporn Chaiyasan ◽

Weerayuth Supiwong ◽

...

Keyword(s):

Chromosome Number ◽

Nucleolar Organizer ◽

Subtelomeric Region ◽

Diploid Chromosome Number ◽

Chromosome Study ◽

Banding Technique ◽

Nucleolar Organizer Regions ◽

Renal Cells ◽

Mitotic Chromosomes ◽

Acrocentric Chromosomes

The cytogenetic comparisons of five Minnow species from Thailand were presented here, i.e., Devario regina, D. laoensis, Rasbora paviana, R. aurotaenia and Esomus metalicus. The mitotic chromosomes were prepared directly from renal cells. Conventional staining and Ag-NOR banding techniques were applied to stain the chromosomes. The results revealed that all Minnow fishes studied possessed the same diploid chromosome number (2n) as 50 chromosomes. The fundamental numbers (NF) of D. laoensis, D. regina, R. paviana, R. aurotaenia and E. metalicus are 100, 100, 98, 98, and 98 respectively. Their karyotypes composing of metacentrics-submetacentrics-acrocentrics-telocentrics were as follows: 6-12-32-0 in D. regina, 6-10-34-0 in D. laoensis, 8-16-24-2 in R. paviana, 8-16-24-2 in R. aurotaenia and 8-10-30-2 in E. metalicus. The Ag-NOR banding technique provides the nucleolar organizer regions (NORs) at subtelomeric region of the short arm chromosome in the a submetacentric or acrocentric chromosomes that are located differently in the different chromosome pairs among species.

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Cytogenetic analysis of the neotropical spider Nephilengys cruentata (Araneomorphae, tetragnathidae): standard staining, NORs, C-bands and base-specific fluorochromes

Brazilian Journal of Biology ◽

10.1590/s1519-69842005000200002 ◽

2005 ◽

Vol 65 (2) ◽

pp. 193-202 ◽

Cited By ~ 23

Author(s):

D. Araújo ◽

D. M. Cella ◽

A. D. Brescovit

Keyword(s):

Sex Determination ◽

Banding Pattern ◽

Constitutive Heterochromatin ◽

Repetitive Sequences ◽

Nucleolar Organizer ◽

Pericentromeric Region ◽

Banding Technique ◽

Nucleolar Organizer Regions ◽

Fluorochrome Staining ◽

C Banding

The aim of this work is to characterize Nephilengys cruentata in relation to the diploid number, chromosome morphology, type of sex determination chromosome system, chromosomes bearing the Nucleolar Organizer Regions (NORs), C-banding pattern, and AT or GC repetitive sequences. The chromosome preparations were submitted to standard staining (Giemsa), NOR silver impregnation, C-banding technique, and base-specific fluorochrome staining. The analysis of the cells showed 2n = 24 and 2n = 26 chromosomes in the embryos, and 2n = 26 in the ovarian cells, being all the chromosomes acrocentric. The long arm of the pairs 1, 2 and 3 showed an extensive negative heteropycnotic area when the mitotic metaphases were stained with Giemsa. The sexual chromosomes did not show differential characteristics that allowed to distinguish them from the other chromosomes of the complement. Considering the diploid numbers found in N. cruentata and the prevalence of X1X2 sex determination chromosome system in Tetragnathidae, N. cruentata seems to possess 2n = 24 = 22 + X1X2 in the males, and 2n = 26 = 22 + X1X1X2X2 in the females. The pairs 1, 2 and 3 showed NORs which are coincident with the negative heteropycnotic patterns. Using the C-banding technique, the pericentromeric region of the chromosomes revealed small quantity or even absence of constitutive heterochromatin, differing of the C-banding pattern described in other species of spiders. In N. cruentata the fluorochromes DAPI/DA, DAPI/MM and CMA3/DA revealed that the constitutive heterochromatin is rich in AT bases and the NORs possess repetitive sequences of GC bases.

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Constitutive heterochromatin DNA polymorphisms in diploid Medicago sativa ssp. falcata

Genome ◽

10.1139/g99-038 ◽

1999 ◽

Vol 42 (5) ◽

pp. 930-935 ◽

Cited By ~ 10

Author(s):

G R Bauchan ◽

M A Hossain

Keyword(s):

Medicago Sativa ◽

Banding Pattern ◽

Constitutive Heterochromatin ◽

Nucleolar Organizer ◽

Dna Polymorphisms ◽

Banding Technique ◽

Chromosome 3 ◽

Nucleolar Organizer Regions ◽

First Report ◽

C Banding

A Giemsa C-banding technique was used to study the amount and location of constitutive heterochromatin in diploid (2n = 2x = 16) Medicago sativa ssp. falcata (L.) Arcangeli. Most accessions had the standard C-banding pattern with centromeric bands on all the chromosomes and a prominent heterochromatic band at the nucleolar organizer regions (NOR) of the satellited (SAT) chromosomes. However, we observed in various accessions that constitutive heterochromatic C-bands can exist at the telomeric ends of all the chromosomes. Interstitial bands occurred on the short arms of all chromosomes except for chromosome 3 and on the long arms of chromosomes 1, 2, 3, and 6, only. Rearranged chromosomes such as isochromosomes have been observed for the short arms of chromosomes 2 and 6. This is the first report on the existence of C-banding polymorphisms and the detection of putative isochromsomes in the chromosomes of diploid ssp. falcata which could have contributed to the variation observed in cultivated alfalfa.Key words: alfalfa, C-bands, chromosome, isochromosome.

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Electron Microscopic investigation of crooke’s change in the pituitaries of patients with hypercorticism

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100156225 ◽

1989 ◽

Vol 47 ◽

pp. 848-849

Author(s):

E. Horvath ◽

K. Kovacs ◽

L. Stefaneanu ◽

N. Losinski

Keyword(s):

Nucleolar Organizer ◽

Microscopic Investigation ◽

Electron Microscopic Investigation ◽

Ectopic Acth Syndrome ◽

Nucleolar Organizer Regions ◽

Electron Microscopic ◽

Human Pituitary ◽

Ectopic Acth ◽

Crooke’S Hyalinization

Human pituitary corticotropins have unique morphologic markers: bundles of type-1 filaments, measuring approximately 70 A in width and representing cytokeratin. The extreme ring-like accumulation of type-1 filaments, known as Crooke's hyalinization, signals functional suppression of the corticotropins and occurs in endogenous and exogenous glucocorticoid excess, caused by ACTH-secreting pituitary adenoma, glucocorticoid secreting adrenocortical tumor, ectopic ACTH-syndrome and administration of pharmacologic doses of glucocorticoids. Cells of autonomous corticotroph adenomas usually do not show Crooke's hyalin change. A minority of these tumors, however, retains sensitivity to the negative feed-back effect of elevated blood glucocorticoid levels and display typical Crooke’s change.In the present study pituitary corticotropins in various phases of Crooke's hyalinization were investigated in patients with glucocorticoid excess of various origin, applying histology, immunocytochemistry, count of argyrophilic nucleolar organizer regions (AgNOR), and transmission electron microscopy.

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A role for upstream binding factor in organizing ribosomal gene chromatin

Biochemical Society Symposium ◽

10.1042/bss0730077 ◽

2006 ◽

Vol 73 ◽

pp. 77-84 ◽

Cited By ~ 14

Author(s):

Jane E. Wright ◽

Christine Mais ◽

José-Luis Prieto ◽

Brian McStay

Keyword(s):

Nucleolar Organizer ◽

Ribosomal Gene ◽

Rna Polymerase I ◽

Nucleolar Organizer Regions ◽

Binding Factor ◽

Upstream Binding Factor ◽

Acrocentric Chromosomes ◽

Polymerase I ◽

Secondary Constrictions ◽

Active Nors

Human ribosomal genes are located in NORs (nucleolar organizer regions) on the short arms of acrocentric chromosomes. During metaphase, previously active NORs appear as prominent chromosomal features termed secondary constrictions, which are achromatic in chromosome banding and positive in silver staining. The architectural RNA polymerase I transcription factor UBF (upstream binding factor) binds extensively across the ribosomal gene repeat throughout the cell cycle. Evidence that UBF underpins NOR structure is provided by an examination of cell lines in which large arrays of a heterologous UBF binding sequences are integrated at ectopic sites on human chromosomes. These arrays efficiently recruit UBF even to sites outside the nucleolus, and during metaphase form novel silver-stainable secondary constrictions, termed pseudo-NORs, that are morphologically similar to NORs.

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Structure and variability of nucleolar organizer regions in Parodontidae fish

Canadian Journal of Genetics and Cytology ◽

10.1139/g84-089 ◽

1984 ◽

Vol 26 (5) ◽

pp. 564-568 ◽

Cited By ~ 51

Author(s):

Orlando Moreira-Filho ◽

Luiz Antonio Carlos Bertollo ◽

Pedro Manoel Galetti Jr.

Keyword(s):

Chromosome Pair ◽

Constitutive Heterochromatin ◽

Nucleolar Organizer ◽

Nucleolar Organizer Regions ◽

Mitotic Chromosomes ◽

Homozygous Condition ◽

Nucleolar Organizing Regions ◽

Nucleolar Chromosome

Nucleolar organizer regions (NORs) were studied in mitotic chromosomes of four species of fish of family Parodontidae: Parodon tortuosus, Apareiodon affinis, Apareiodon ibitiensis, and Apareiodon piracicabae. All four species exhibited only a single nucleolar chromosome pair in their karyotypes. Intraspecific differences were observed in the size of these chromosomes; however, these were not very clear for A. affinis and A. piracicabae, Apareiodon piracicabae exhibited two clearly visible NORs in each of the nucleolar chromosomes, which was the only configuration practically found in this species. This trait therefore predominates in a homozygous condition in the population investigated. Regions of constitutive heterochromatin adjacent to the two NORs were detected. Possible mechanisms that may have originated the two NORs are discussed.Key words: nucleolar organizing regions, fish.

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