Recombinant Bovine Herpesvirus-1 (BHV-1) Lacking Transactivator Protein BICPO Entails Lack of Glycoprotein C and Severely Reduced Infectivity

1996 ◽  
Vol 377 (12) ◽  
pp. 787-796 ◽  
Author(s):  
René Köppel ◽  
Cornel Fraefel ◽  
Bernd Vogt ◽  
Leonard J. Bello ◽  
William C. Lawrence ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein C ◽  
Herpesvirus 1 ◽  
Transactivator Protein

Induction of immune responses in cattle with a DNA vaccine encoding glycoprotein C of bovine herpesvirus-1

2001 ◽  
Vol 78 (4) ◽  
pp. 293-305 ◽  
Author(s):  
Praveen K. Gupta ◽  
Mohini Saini ◽  
L.K. Gupta ◽  
V.D.P. Rao ◽  
S.K. Bandyopadhyay ◽  
...  
Keyword(s):  
Immune Responses ◽  
Dna Vaccine ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein C ◽  
Herpesvirus 1

scholarly journals Cloning and expression of bovine herpesvirus-1 glycoprotein C

IUBMB Life ◽  
1999 ◽  
Vol 47 (2) ◽  
pp. 275-282
Author(s):  
Praveen Gupta ◽  
Mohini Saini ◽  
L. K. Gupta ◽  
S. K. Bandyopadhyay ◽  
S. K. Garg
Keyword(s):  
Bovine Herpesvirus ◽  
Cloning And Expression ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein C ◽  
Herpesvirus 1

scholarly journals Transactivator Protein BICP0 of Bovine Herpesvirus 1 (BHV-1) Is Blocked by Prostaglandin D2 (PGD2), Which Points to a Mechanism for PGD2-Mediated Inhibition of BHV-1 Replication

2004 ◽  
Vol 78 (8) ◽  
pp. 3805-3810 ◽  
Author(s):  
Okay Saydam ◽  
Carlos Abril ◽  
Bernd Vogt ◽  
Mathias Ackermann ◽  
Martin Schwyzer
Keyword(s):  
Cultured Cells ◽  
Bovine Herpesvirus ◽  
Prostaglandin D2 ◽  
Bovine Herpesvirus 1 ◽  
Early Protein ◽  
Cellular Genes ◽  
Cellular Target ◽  
Antiviral Activities ◽  
Herpesvirus 1 ◽  
Transactivator Protein

ABSTRACT The immediate-early protein, BICP0, of bovine herpesvirus 1 (BHV-1) transactivates a variety of viral and cellular genes. In a yeast two-hybrid cDNA library screening, we found that lipocalin-type prostaglandin D synthase, which catalyzes the production of prostaglandin D2 (PGD2), is a cellular target of BICP0. We observed that, during wild-type BHV-1 infection, PGD2 levels were increased intracellularly and decreased in the medium. These effects were absent upon infection with recombinant BHV-1 expressing β-galactosidase instead of BICP0 (A2G2). Transient-expression assays showed that BICP0 alone caused a significant increase in PGD2 levels in the cell. PGD2 repressed BHV-1 replication in cultured cells. Antiviral activities of prostaglandins have been documented long ago, but their mode of action remains to be clarified. Here we provide evidence that PGD2 impairs the transactivation ability of BICP0 that is necessary for efficient virus replication.

Rapid detection and differentiation of bovine herpesvirus 1 and 5 glycoprotein C gene in clinical specimens by multiplex-PCR

2005 ◽  
Vol 128 (1-2) ◽  
pp. 183-188 ◽  
Author(s):  
Marlise Pompeo Claus ◽  
Alice Fernandes Alfieri ◽  
Áurea Valadares Folgueras-Flatschart ◽  
Sheila Rezler Wosiacki ◽  
Kerlei Cristina Médici ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Rapid Detection ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Clinical Specimens ◽  
Glycoprotein C ◽  
Herpesvirus 1

scholarly journals Epitopes on glycoprotein C of bovine herpesvirus-1 (BHV-1) that allow differentiation between BHV-1.1 and BHV-1.2 strains.

1999 ◽  
Vol 80 (6) ◽  
pp. 1477-1483 ◽  
Author(s):  
F A Rijsewijk ◽  
M J Kaashoek ◽  
J P Langeveld ◽  
R Meloen ◽  
J Judek ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein C ◽  
Herpesvirus 1

scholarly journals Rise and Survival of Bovine Herpesvirus 1 Recombinants after Primary Infection and Reactivation from Latency

2003 ◽  
Vol 77 (23) ◽  
pp. 12535-12542 ◽  
Author(s):  
Frédéric Schynts ◽  
François Meurens ◽  
Bruno Detry ◽  
Alain Vanderplasschen ◽  
Etienne Thiry
Keyword(s):  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein C ◽  
Recombinant Viruses ◽  
Reactivation Treatment ◽  
Frequent Event ◽  
Herpesvirus 1

ABSTRACT Recombination is thought to be an important source of genetic variation in herpesviruses. Several studies, performed in vitro or in vivo, detected recombinant viruses after the coinoculation of two distinguishable strains of the same herpesvirus species. However, none of these studies investigated the evolution of the relative proportions of parental versus recombinant progeny populations after coinoculation of the natural host, both during the excretion and the reexcretion period. In the present study, we address this by studying the infection of cattle with bovine herpesvirus 1 (BoHV-1). The recombination of two BoHV-1 mutants lacking either glycoprotein C (gC−/gE+) or E (gC+/gE−) was investigated after inoculation of cattle by the natural route of infection. The results demonstrated that (i) recombination is a frequent event in vivo since recombinants (gC+/gE+ and gC−/gE−) were detected in all coinoculated calves, (ii) relative proportions of progeny populations evolved during the excretion period toward a situation where two populations (gC+/gE+ and gC−/gE+) predominated without fully outcompeting the presence of the two other detected populations (gC+/gE− and gC−/gE−), and (iii) after reactivation from latency, no gC+/gE− and gC−/gE− progeny viruses were detected, although gC+/gE− mutants, when inoculated alone, were detected after reactivation treatment. In view of these data, the importance of gE in the biology of BoHV-1 infection and the role of recombination in herpesvirus evolution are discussed.

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