Rapid detection and differentiation of bovine herpesvirus 1 and 5 glycoprotein C gene in clinical specimens by multiplex-PCR

2005 ◽  
Vol 128 (1-2) ◽  
pp. 183-188 ◽  
Author(s):  
Marlise Pompeo Claus ◽  
Alice Fernandes Alfieri ◽  
Áurea Valadares Folgueras-Flatschart ◽  
Sheila Rezler Wosiacki ◽  
Kerlei Cristina Médici ◽  
...  
Keyword(s):  
Multiplex Pcr ◽  
Rapid Detection ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Clinical Specimens ◽  
Glycoprotein C ◽  
Herpesvirus 1

Induction of immune responses in cattle with a DNA vaccine encoding glycoprotein C of bovine herpesvirus-1

2001 ◽  
Vol 78 (4) ◽  
pp. 293-305 ◽  
Author(s):  
Praveen K. Gupta ◽  
Mohini Saini ◽  
L.K. Gupta ◽  
V.D.P. Rao ◽  
S.K. Bandyopadhyay ◽  
...  
Keyword(s):  
Immune Responses ◽  
Dna Vaccine ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein C ◽  
Herpesvirus 1

Recombinant Bovine Herpesvirus-1 (BHV-1) Lacking Transactivator Protein BICPO Entails Lack of Glycoprotein C and Severely Reduced Infectivity

1996 ◽  
Vol 377 (12) ◽  
pp. 787-796 ◽  
Author(s):  
René Köppel ◽  
Cornel Fraefel ◽  
Bernd Vogt ◽  
Leonard J. Bello ◽  
William C. Lawrence ◽  
...  
Keyword(s):  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein C ◽  
Herpesvirus 1 ◽  
Transactivator Protein

scholarly journals Cloning and expression of bovine herpesvirus-1 glycoprotein C

IUBMB Life ◽  
1999 ◽  
Vol 47 (2) ◽  
pp. 275-282
Author(s):  
Praveen Gupta ◽  
Mohini Saini ◽  
L. K. Gupta ◽  
S. K. Bandyopadhyay ◽  
S. K. Garg
Keyword(s):  
Bovine Herpesvirus ◽  
Cloning And Expression ◽  
Bovine Herpesvirus 1 ◽  
Glycoprotein C ◽  
Herpesvirus 1

Rapid detection of bovine herpesvirus 1 in bovine semen by loop-mediated isothermal amplification (LAMP) assay

2013 ◽  
Vol 159 (4) ◽  
pp. 641-648 ◽  
Author(s):  
Sachin S. Pawar ◽  
Chetan D. Meshram ◽  
Niraj K. Singh ◽  
Arvind A. Sonwane ◽  
Mohini Saini ◽  
...  
Keyword(s):  
Rapid Detection ◽  
Lamp Assay ◽  
Bovine Herpesvirus ◽  
Isothermal Amplification ◽  
Bovine Herpesvirus 1 ◽  
Bovine Semen ◽  
Loop Mediated Isothermal Amplification ◽  
Herpesvirus 1

scholarly journals A new molecular method for the rapid subtyping of bovine herpesvirus 1 field isolates

2020 ◽  
Vol 32 (1) ◽  
pp. 112-117
Author(s):  
Silvina S. Maidana ◽  
Samuel Miño ◽  
Romina M. Apostolo ◽  
Gabriel A. De Stefano ◽  
Sonia A. Romera
Keyword(s):  
Multiplex Pcr ◽  
Rapid Test ◽  
Bovine Herpesvirus ◽  
Restriction Endonuclease Analysis ◽  
Snp Analysis ◽  
Hindiii Site ◽  
Bovine Herpesvirus 1 ◽  
Clinical Syndromes ◽  
Herpesvirus 1

Bovine herpesvirus 1 (BoHV-1) causes several clinical syndromes in cattle worldwide. There are 3 subtypes of BoHV-1: 1.1, 1.2a, and 1.2b. Several molecular methods are commonly used in the detection and characterization of BoHV-1. Among them, restriction endonuclease analysis (REA) and single-nucleotide polymorphism (SNP) analysis of the complete viral genome allow classification of BoHV-1 into different subtypes. However, developing countries need simpler and cheaper screening assays for routine testing. We designed a standard multiplex PCR followed by a REA assay allowing straightforward subclassification of all BoHV-1 isolates tested into 1.1, 1.2a, and 1.2b subtypes based on the analysis of fragment length polymorphism. Our standard multiplex PCR-REA was used to analyze 33 field strains of BoHV-1 isolated from various tissues. The assay confirmed the subtype identified previously by REA. In addition, non-polymorphic or undigested fragments were sequenced in order to confirm the mutation affecting the RE HindIII site. Our PCR-REA method is an affordable and rapid test that will subtype all BoHV-1 strains.

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