Value-added reporting of antinuclear antibody testing by automated indirect immunofluorescence analysis

2014 ◽  
Vol 52 (4) ◽  
Author(s):  
Sofie Schouwers ◽  
Myriam Bonnet ◽  
Patrick Verschueren ◽  
René Westhovens ◽  
Daniel Blockmans ◽  
...  
Keyword(s):  
Indirect Immunofluorescence ◽  
Antinuclear Antibody ◽  
Value Added ◽  
Antibody Testing ◽  
Immunofluorescence Analysis

Reconstructing a 3-dimensional image of the results of antinuclear antibody testing by indirect immunofluorescence

2013 ◽  
Vol 387 (1-2) ◽  
pp. 312-316
Author(s):  
Ryosei Murai ◽  
Koji Yamada ◽  
Maki Tanaka ◽  
Kageaki Kuribayashi ◽  
Daisuke Kobayashi ◽  
...  
Keyword(s):  
Indirect Immunofluorescence ◽  
Antinuclear Antibody ◽  
Antibody Testing ◽  
3 Dimensional ◽  
Dimensional Image

scholarly journals Variation in antinuclear antibody detection by automated indirect immunofluorescence analysis

2018 ◽  
Vol 78 (6) ◽  
pp. e48-e48 ◽  
Author(s):  
Lieve Van Hoovels ◽  
Sofie Schouwers ◽  
Stefanie Van den Bremt ◽  
Xavier Bossuyt
Keyword(s):  
Indirect Immunofluorescence ◽  
Antinuclear Antibody ◽  
Antibody Detection ◽  
Immunofluorescence Analysis

scholarly journals The role of NbTMP1, a surface protein of sporoplasm, in Nosema bombycis infection

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Shiyi Zheng ◽  
Yukang Huang ◽  
Hongyun Huang ◽  
Bin Yu ◽  
Ni Zhou ◽  
...  
Keyword(s):  
Indirect Immunofluorescence ◽  
Selective Breeding ◽  
Developmental Stages ◽  
Transmembrane Protein ◽  
Surface Protein ◽  
Spore Wall ◽  
Significant Inhibition ◽  
Pcr Analysis ◽  
Immunofluorescence Analysis ◽  
Nosema Bombycis

Abstract Background Nosema bombycis is a unicellular eukaryotic pathogen of the silkworm, Bombyx mori, and is an economic and occupational hazard in the silkworm industry. Because of its long incubation period and horizontal and vertical transmission, it is subject to quarantine measures in sericulture production. The microsporidian life-cycle includes a dormant extracellular phase and intracellular proliferation phase, with the proliferation period being the most active period. This latter period lacks spore wall protection and may be the most susceptible stage for control. Methods In order to find suitable target for the selective breeding of N. bombycis-resistant silkworm strains, we screen highly expressed membrane proteins from the transcriptome data of N. bombycis. The subcellular localization of the candidate protein was verified by Indirect immunofluorescence analysis (IFA) and immunoelectron microscopy (IEM), and its role in N. bombycis proliferation was verified by RNAi. Results The N. bombycis protein (NBO_76g0014) was identified as a transmembrane protein and named NbTMP1. It is homologous with hypothetical proteins NGRA_1734 from Nosema granulosis. NbTMP1 has a transmembrane region of 23 amino acids at the N-terminus. Indirect immunofluorescence analysis (IFA) results suggest that NbTMP1 is secreted on the plasma membrane as the spores develop. Western blot and qRT-PCR analysis showed that NbTMP1 was expressed in all developmental stages of N. bombycis in infected cells and in the silkworm midgut. Downregulation of NbTMP1 expression resulted in significant inhibition of N. bombycis proliferation. Conclusions We confirmed that NbTMP1 is a membrane protein of N. bombycis. Reduction of the transcription level of NbTMP1 significantly inhibited N. bombycis proliferation, and this protein may be a target for the selective breeding of N. bombycis-resistant silkworm strains.

Antinuclear antibody testing: discordance between commercial laboratories

2016 ◽  
Vol 35 (7) ◽  
pp. 1713-1718 ◽  
Author(s):  
Aryeh M. Abeles ◽  
Manuel Gomez-Ramirez ◽  
Micha Abeles ◽  
Shyoko Honiden
Keyword(s):  
Antinuclear Antibody ◽  
Antibody Testing

Measurements of Endpoint Titers Based on the Fluorescence Intensity Trend in Anti-Nuclear Antibody Testing

2019 ◽  
Vol 51 (5) ◽  
pp. 469-477 ◽  
Author(s):  
Dong I L Won
Keyword(s):  
Fluorescence Intensity ◽  
Error Rate ◽  
Antinuclear Antibody ◽  
Regression Line ◽  
Cost Effective ◽  
Serial Dilution ◽  
Automated Systems ◽  
Antibody Testing ◽  
Single Well ◽  
Line Slope

Abstract Background Automated systems for antinuclear antibody (ANA) testing provide endpoint titers that are predicted based on the fluorescence intensity (FI) value at a screening dilution (single-well titration [SWT]) showing frequent titration errors (more than plus or minus 1 dilution). Methods Line slope titration (LST) was based on the trend of FI values on dilutions. Three dilutions per specimen were prepared considering a patient’s previous titer or FI at the screening dilution. On the XY plot, with the reciprocal of dilution as the X-axis and FI value as the Y-axis, a fitted line was drawn to obtain the endpoint titers. Results The titration error rate (no. of errors/total no.) of LST using a regression line was lower than that of SWT (31/710 [4.4%] and 152/674 [22.6%], respectively; P < .000000001), with serial dilution as a reference. When comparing a regression line using 3 dilution points with a line using 2 dilution points, the error rate of the former was not significantly different from that of the latter (31/710 [4.4%] and 31/746 [4.2%], respectively; P = .842). Conclusions This LST method is useful as an accurate, cost-effective, and rapid approach to measure endpoint titers in routine ANA testing.

Antinuclear antibody testing

2002 ◽  
Vol 22 (2) ◽  
pp. 447-474 ◽  
Author(s):  
David F Keren
Keyword(s):  
Antinuclear Antibody ◽  
Antibody Testing

scholarly journals Current laboratory and clinical practices in reporting and interpreting anti-nuclear antibody indirect immunofluorescence (ANA IIF) patterns: results of an international survey

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Lieve Van Hoovels ◽  
Sylvia Broeders ◽  
Edward K. L. Chan ◽  
Luis Andrade ◽  
Wilson de Melo Cruvinel ◽  
...  
Keyword(s):  
Indirect Immunofluorescence ◽  
Clinical Relevance ◽  
Clinical Practices ◽  
Antibody Testing ◽  
Clinical Value ◽  
International Consensus ◽  
Nuclear Antigens ◽  
Dsdna Antibody ◽  
Homogeneous Pattern ◽  
Rods And Rings

Abstract Background The International Consensus on Antinuclear Antibody (ANA) Patterns (ICAP) has recently proposed nomenclature in order to harmonize ANA indirect immunofluorescence (IIF) pattern reporting. ICAP distinguishes competent-level from expert-level patterns. A survey was organized to evaluate reporting, familiarity, and considered clinical value of ANA IIF patterns. Methods Two surveys were distributed by European Autoimmunity Standardization Initiative (EASI) working groups, the International Consensus on ANA Patterns (ICAP) and UK NEQAS to laboratory professionals and clinicians. Results 438 laboratory professionals and 248 clinicians from 67 countries responded. Except for dense fine speckled (DFS), the nuclear competent patterns were reported by > 85% of the laboratories. Except for rods and rings, the cytoplasmic competent patterns were reported by > 72% of laboratories. Cytoplasmic IIF staining was considered ANA positive by 55% of clinicians and 62% of laboratory professionals, with geographical and expertise-related differences. Quantification of fluorescence intensity was considered clinically relevant for nuclear patterns, but less so for cytoplasmic and mitotic patterns. Combining IIF with specific extractable nuclear antigens (ENA)/dsDNA antibody testing was considered most informative. Of the nuclear competent patterns, the centromere and homogeneous pattern obtained the highest scores for clinical relevance and the DFS pattern the lowest. Of the cytoplasmic patterns, the reticular/mitochondria-like pattern obtained the highest scores for clinical relevance and the polar/Golgi-like and rods and rings patterns the lowest. Conclusion This survey confirms that the major nuclear and cytoplasmic ANA IIF patterns are considered clinically important. There is no unanimity on classifying DFS, rods and rings and polar/Golgi-like as a competent pattern and on reporting cytoplasmic patterns as ANA IIF positive.

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