Serum Proteins by Capillary Zone Electrophoresis: Approaches to the Definition of Reference Values

1999 ◽  
Vol 37 (10) ◽  
Author(s):  
Concetta Petrini ◽  
Maria G. Alessio ◽  
Luisa Scapellato ◽  
Simona Brambilla ◽  
Carlo Franzini
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Reference Values ◽  
Serum Proteins ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Definition Of

scholarly journals Serum Proteins Capillary Zone Electrophoresis: A Comparison of Two Systems

2006 ◽  
Vol 37 (4) ◽  
pp. 233-236 ◽  
Author(s):  
Alessandro Terreni ◽  
Anna Caldini ◽  
Agostino Ognibene ◽  
Benedetta Salvadori ◽  
Gianni Messeri
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Serum Proteins ◽  
Zone Electrophoresis ◽  
Two Systems ◽  
Capillary Zone

Determination of denatured serum proteins in the casein fraction of heat-treated milk by capillary zone electrophoresis

1996 ◽  
Vol 17 (7) ◽  
pp. 1228-1233 ◽  
Author(s):  
Isidra Recio ◽  
Cornelis Olieman
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Serum Proteins ◽  
Heat Treated ◽  
Zone Electrophoresis ◽  
Capillary Zone

Sensitivity investigation of high resolution capillary zone electrophoresis for serum proteins (CZE-HR SPE) for slight monoclonal bands as compared to Hevylite™

2012 ◽  
Vol 45 (13-14) ◽  
pp. 1106
Author(s):  
J.-P. Émond ◽  
T. El Hariri ◽  
N. Fourrier ◽  
J. Burden ◽  
S. Harding
Keyword(s):  
High Resolution ◽  
Capillary Zone Electrophoresis ◽  
Serum Proteins ◽  
Zone Electrophoresis ◽  
Capillary Zone

Capillary Zone Electrophoresis of Serum Proteins: Effects of Changed Analytical Conditions

2003 ◽  
Vol 41 (6) ◽  
Author(s):  
Paola Luraschi ◽  
Elisabetta Dalla Dea ◽  
Carlo Franzini
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Serum Proteins ◽  
Zone Electrophoresis ◽  
Capillary Zone

scholarly journals Effect of Sulfamethoxazole on Clinical Capillary Zone Electrophoresis of Serum Proteins

2003 ◽  
Vol 49 (2) ◽  
pp. 340-341 ◽  
Author(s):  
Xavier Bossuyt ◽  
Jan Verhaegen ◽  
Godelieve Mariën ◽  
Norbert Blanckaert
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Serum Proteins ◽  
Zone Electrophoresis ◽  
Capillary Zone

Determination of poorly separated monoclonal serum proteins by capillary zone electrophoresis

2002 ◽  
Vol 775 (1) ◽  
pp. 63-70 ◽  
Author(s):  
T LEBRICON ◽  
E LAUNAY ◽  
P HOUZE ◽  
D BENGOUFA ◽  
B BOUSQUET ◽  
...  
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Serum Proteins ◽  
Zone Electrophoresis ◽  
Capillary Zone

EXPRESS: The effect of paraprotein polymerisation on quantitation by capillary zone electrophoresis and Hevylite®

2021 ◽  
pp. 000456322110293
Author(s):  
Helen Valentine ◽  
Anne Dawnay
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Serum Proteins ◽  
Reducing Agent ◽  
Single Band ◽  
Post Treatment ◽  
Close Proximity ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Biclonal Gammopathy ◽  
Turbidimetric Assay

Objectives Up to 3% of patients with monoclonal gammopathies have multiple serum paraproteins. This article investigates whether multiple isotype-matched paraproteins, as seen on capillary zone electrophoresis (CZE), are truly biclonal. Methods Sera containing multiple isotype-matched paraproteins were treated with the reducing agent dithiothreitol (DTT) and CZE performed pre- and post-treatment. Band resolution and effect of resolution on quantitation of paraprotein burden was assessed. The Hevylite® turbidimetric assay was also evaluated for ability to quantify such paraproteins. Results Among patients with biclonal isotype-matched (BIM) paraproteins, 23/24 (96%) IgA paraproteins resolved into a single band following treatment with DTT compared with only 1/12 (8%) IgG paraproteins. Daratumumab therapy accounted for the second band in 5/9 non-resolving IgGκ paraproteins. Where initially quantified as a single IgA “complex” (multiple bands in close proximity), the single post-DTT band averaged 2.8 g/L less (P=<0.001), likely due to inclusion of lower amounts of underlying serum proteins (y=0.97x-2.03, R2=0.993). Quantitating IgA BIM (n=58) using the Hevylite® assay gave higher results (P=0.002) than CZE (y=1.48x–7.13, R2=0.959). In contrast, single IgA paraprotein results (n=48) did not differ between the two methods (P=0.466; y=1.24x – 2.74, R2=0.898), suggesting that polymerisation enhances Hevylite® quantitation. Conclusions These results suggest that disulphide-mediated polymerisation of IgA paraproteins is more common than true biclonal gammopathy and support DTT treatment of samples with isotype-matched IgA bands before quantifying by CZE. The Hevylite® assay should be utilised with caution where polymerisation is likely. Where IgGκ BIM paraproteins appear on CZE, Daratumumab therapy should be considered.

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