Steroid binding properties of the 2nd WHO International Standard for sex hormone-binding globulin

2011 ◽  
Vol 49 (5) ◽  
Author(s):  
Markus Thaler ◽  
Carolin Müller ◽  
Alice Schlichtiger ◽  
Katharina Gründler ◽  
Melanie Moore ◽  
...  
Keyword(s):  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Hormone Binding ◽  
Binding Properties ◽  
International Standard ◽  
Steroid Binding

Resolution of the steroid-binding and dimerization domains of human sex hormone-binding globulin by expression in Escherichia coli

Biochemistry ◽  
1995 ◽  
Vol 34 (10) ◽  
pp. 3231-3238 ◽  
Author(s):  
Catherine Hildebrand ◽  
Wayne P. Bocchinfuso ◽  
David Dales ◽  
Geoffrey L. Hammond
Keyword(s):  
Escherichia Coli ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Hormone Binding ◽  
Expression In Escherichia Coli ◽  
Steroid Binding

scholarly journals Resolution of the Human Sex Hormone-binding Globulin Dimer Interface and Evidence for Two Steroid-binding Sites per Homodimer

2001 ◽  
Vol 276 (37) ◽  
pp. 34453-34457 ◽  
Author(s):  
George V. Avvakumov ◽  
Irina Grishkovskaya ◽  
Yves A. Muller ◽  
Geoffrey L. Hammond
Keyword(s):  
Binding Sites ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Hormone Binding ◽  
Dimer Interface ◽  
Steroid Binding

The cDNA-deduced primary structure of human sex hormone-binding globulin and location of its steroid-binding domain

FEBS Letters ◽  
1987 ◽  
Vol 215 (1) ◽  
pp. 100-104 ◽  
Author(s):  
G.L. Hammond ◽  
D.A. Underhill ◽  
C.L. Smith ◽  
I.S. Goping ◽  
M.J. Harley ◽  
...  
Keyword(s):  
Primary Structure ◽  
Binding Domain ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Hormone Binding ◽  
Steroid Binding

Resolution of a Disordered Region at the Entrance of the Human Sex Hormone-binding Globulin Steroid-binding Site

2002 ◽  
Vol 318 (3) ◽  
pp. 621-626 ◽  
Author(s):  
Irina Grishkovskaya ◽  
George V Avvakumov ◽  
Geoffrey L Hammond ◽  
Yves A Muller
Keyword(s):  
Binding Site ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Hormone Binding ◽  
Steroid Binding

Time-resolved immunofluorometric assay of sex-hormone binding globulin.

1988 ◽  
Vol 34 (1) ◽  
pp. 63-66 ◽  
Author(s):  
S Niemi ◽  
O Mäentausta ◽  
N J Bolton ◽  
G L Hammond
Keyword(s):  
Binding Capacity ◽  
Reference Intervals ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Hormone Binding ◽  
Time Resolved ◽  
Interassay Coefficient ◽  
The Mean ◽  
Steroid Binding ◽  
The Eu

Abstract A time-resolved immunofluorometric assay (trlFMA) for human sex-hormone binding globulin (SHBG) is described in which antibody-coated tubes or microliter strip-wells and a europium (Eu) chelate-labeled monoclonal antibody are used. The trlFMA sensitivity is similar to that of other SHBG immunoassays, and other analytical variables compare favorably with an SHBG immunoradiometric assay (IRMA) kit and a steroid binding capacity assay: the interassay coefficient of variation (CV) is less than 8% and the intra-assay CV is less than 6% for concentrations between 6 and 200 nmol/L. The reference intervals (means +/- SD) for SHBG concentrations (nmol/L) in serum from 10 men, 10 women, and 10 pregnant women were 23 +/- 12, 65 +/- 39, and 439 +/- 122, respectively. In 14 hirsute women the mean +/- SD serum SHBG concentration (37 +/- 21 nmol/L) was significantly lower (P less than 0.01) than the mean for an age-matched, nonhirsute female comparison group. The trlFMA is technically simple, requires no centrifugation or separation reagent, and takes a counting time of only 1 s. In addition, the Eu-label is nontoxic, presents no waste-disposal problems, and has a long shelf life.

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