Flow chemistry approaches directed at improving chemical synthesis

2013 ◽  
Vol 2 (3) ◽  
Author(s):  
Ian R. Baxendale ◽  
Laurens Brocken ◽  
Carl J. Mallia
Keyword(s):  
Chemical Synthesis ◽  
Flow Chemistry

scholarly journals Catalytic Methods in Flow Chemistry

Catalysts ◽  
2019 ◽  
Vol 9 (8) ◽  
pp. 663
Author(s):  
Christophe Len ◽  
Renzo Luisi
Keyword(s):  
Chemical Synthesis ◽  
Continuous Flow ◽  
Flow Chemistry ◽  
Pharmaceutical Companies ◽  
Enabling Technology ◽  
Continuous Flow Chemistry ◽  
The Way

Continuous flow chemistry is radically changing the way of performing chemical synthesis, and several chemical and pharmaceutical companies are now investing in this enabling technology [...]

scholarly journals Synthesis of proteins by automated flow chemistry

Science ◽  
2020 ◽  
Vol 368 (6494) ◽  
pp. 980-987 ◽  
Author(s):  
N. Hartrampf ◽  
A. Saebi ◽  
M. Poskus ◽  
Z. P. Gates ◽  
A. J. Callahan ◽  
...  
Keyword(s):  
Amino Acids ◽  
Chemical Synthesis ◽  
Flow Chemistry ◽  
Chain Elongation ◽  
High Fidelity ◽  
Structural Units ◽  
Regulatory Factors ◽  
Synthetic Materials ◽  
Consecutive Reactions ◽  
Fast Flow

Ribosomes can produce proteins in minutes and are largely constrained to proteinogenic amino acids. Here, we report highly efficient chemistry matched with an automated fast-flow instrument for the direct manufacturing of peptide chains up to 164 amino acids long over 327 consecutive reactions. The machine is rapid: Peptide chain elongation is complete in hours. We demonstrate the utility of this approach by the chemical synthesis of nine different protein chains that represent enzymes, structural units, and regulatory factors. After purification and folding, the synthetic materials display biophysical and enzymatic properties comparable to the biologically expressed proteins. High-fidelity automated flow chemistry is an alternative for producing single-domain proteins without the ribosome.

scholarly journals Flow chemistry on the International Space Station: Novel approaches to chemical synthesis and fully automated microreactor systems

2020 ◽  
Author(s):  
Dominique Ebony Williams ◽  
Jana Stoudemire ◽  
Danielle Rosales ◽  
Paul Kuehl ◽  
Gentry Barnett ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
International Space Station ◽  
Space Station ◽  
Flow Chemistry ◽  
International Space ◽  
Novel Approaches

scholarly journals Flow-based Methods in Chemical Peptide and Protein Synthesis

2021 ◽  
Vol 75 (6) ◽  
pp. 480-483
Author(s):  
Kevin Schiefelbein ◽  
Nina Hartrampf
Keyword(s):  
Protein Synthesis ◽  
Chemical Synthesis ◽  
Flow Chemistry ◽  
Synthesis Methods ◽  
Powerful Method ◽  
Time Resolved ◽  
Peptide Cyclization ◽  
Peptide Chemistry ◽  
On Demand ◽  
Future Developments

Flow chemistry has emerged as a powerful method for on-demand chemical synthesis and modification of peptides and proteins. Herein, we discuss the characteristics of flow chemistry and how they are applied to various aspects of peptide chemistry. We highlight recent advances in automated flow-based peptide synthesis, which extend the length of peptides routinely accessible to single-domain proteins and allow for the collection of time-resolved synthesis data. Applications of this data for the prediction of synthesis outcome and the potential for the development of more sustainable synthesis methods are also discussed. Finally, we will review solutionphase approaches, including flow-based ligation strategies and peptide cyclization. Throughout this review, the current challenges and potential future developments are highlighted.

Continuous flow chemistry: where are we now? Recent applications, challenges and limitations

2018 ◽  
Vol 54 (99) ◽  
pp. 13894-13928 ◽  
Author(s):  
Faith M. Akwi ◽  
Paul Watts
Keyword(s):  
Chemical Synthesis ◽  
Continuous Flow ◽  
Flow Chemistry ◽  
Continuous Flow Chemistry

A general outlook of the changing face of chemical synthesis is provided in this article through recent applications of continuous flow processing in both industry and academia.

scholarly journals Flow chemistry on the International Space Station: Novel approaches to chemical synthesis and fully automated microreactor systems

2020 ◽  
Author(s):  
Dominique Ebony Williams ◽  
Jana Stoudemire ◽  
Danielle Rosales ◽  
Paul Kuehl ◽  
Gentry Barnett ◽  
...  
Keyword(s):  
Chemical Synthesis ◽  
International Space Station ◽  
Space Station ◽  
Flow Chemistry ◽  
International Space ◽  
Novel Approaches

scholarly journals Synthesis of Proteins by Automated Flow Chemistry

2020 ◽  
Author(s):  
Nina Hartrampf ◽  
Azin Saebi ◽  
Mackenzie Poskus ◽  
Zachary P. Gates ◽  
Alexander J. Callahan ◽  
...  
Keyword(s):  
Amino Acids ◽  
Chemical Synthesis ◽  
Flow Chemistry ◽  
Living Cells ◽  
Chain Elongation ◽  
High Fidelity ◽  
Structural Units ◽  
Synthetic Materials ◽  
Consecutive Reactions ◽  
Fast Flow

<p>Ribosomes produce most proteins of living cells in seconds. Here we report highly efficient chemistry matched with an automated fast-flow instrument for the direct manufacturing of peptide chains up to 164 amino acids over 328 consecutive reactions. The machine is rapid - the peptide chain elongation is complete in hours. We demonstrate the utility of this approach by the chemical synthesis of nine different protein chains that represent enzymes, structural units, and regulatory factors. After purification and folding, the synthetic materials display biophysical and enzymatic properties comparable to the biologically expressed proteins. High-fidelity automated flow chemistry is an alternative for producing single-domain proteins without the ribosome.<i></i></p>

scholarly journals Synthesis of Proteins by Automated Flow Chemistry

2020 ◽  
Author(s):  
Nina Hartrampf ◽  
Azin Saebi ◽  
Mackenzie Poskus ◽  
Zachary P. Gates ◽  
Alexander J. Callahan ◽  
...  
Keyword(s):  
Amino Acids ◽  
Chemical Synthesis ◽  
Flow Chemistry ◽  
Living Cells ◽  
Chain Elongation ◽  
High Fidelity ◽  
Structural Units ◽  
Synthetic Materials ◽  
Consecutive Reactions ◽  
Fast Flow

<p>Ribosomes produce most proteins of living cells in seconds. Here we report highly efficient chemistry matched with an automated fast-flow instrument for the direct manufacturing of peptide chains up to 164 amino acids over 328 consecutive reactions. The machine is rapid - the peptide chain elongation is complete in hours. We demonstrate the utility of this approach by the chemical synthesis of nine different protein chains that represent enzymes, structural units, and regulatory factors. After purification and folding, the synthetic materials display biophysical and enzymatic properties comparable to the biologically expressed proteins. High-fidelity automated flow chemistry is an alternative for producing single-domain proteins without the ribosome.<i></i></p>

Continuous-flow chemistry toward sustainable chemical synthesis

2020 ◽  
pp. 49-69
Author(s):  
Anderson R. Aguillón ◽  
Marco A. de M. Bezerra ◽  
Mauro R.B.P. Gomez ◽  
Rodrigo O.M.A. de Souza
Keyword(s):  
Chemical Synthesis ◽  
Continuous Flow ◽  
Flow Chemistry ◽  
Continuous Flow Chemistry
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