Diterpenes from Xylopia langsdorffiana Inhibit Cell Growth and Induce Differentiation in Human Leukemia Cells

Two new diterpenes were isolated from stems and leaves of Xylopia langsdorffi ana, entatisane- 7α,16α-diol (xylodiol) and ent-7α-acetoxytrachyloban-18-oic acid (trachylobane), along with the known 8(17),12E,14-labdatrien-18-oic acid (labdane). We investigated their antitumour effects on HL60, U937 and K562 human leukemia cell lines. We found that xylodiol was the most potent diterpene in inhibiting cell proliferation of HL60, U937 and K562 cells, with mean IC50 values of 90, 80 and 50 μM, respectively. Based on the nitroblue tetrazolium (NBT) reduction assay, all the diterpenes were found to induce terminal differentiation in HL60 and K562 cells, with xylodiol being the most effective. NBT reduction was increased by almost 120% after 12 h exposure of HL60 cells to xylodiol at a concentration lower than the IC50 (50 μM). Thus, xylodiol inhibited human leukemia cell growth in vitro partly by inducing cell differentiation, and merits further studies to examine its mechanism of action as a potential antitumoural agent

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In vitro radiosensitivity of human leukemia cell lines.

Radiology ◽

10.1148/radiology.139.2.6939019 ◽

1981 ◽

Vol 139 (2) ◽

pp. 485-487 ◽

Cited By ~ 38

Author(s):

R R Weichselbaum ◽

J S Greenberger ◽

A Schmidt ◽

A Karpas ◽

W C Moloney ◽

...

Keyword(s):

Cell Lines ◽

Leukemia Cell ◽

Human Leukemia ◽

Human Leukemia Cell ◽

Leukemia Cell Lines

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Biofabrication of polyphenols coated Nano palladium and its in-vitro cytotoxicity against human leukemia cell lines (K562)

Journal of Photochemistry and Photobiology B Biology ◽

10.1016/j.jphotobiol.2017.07.025 ◽

2017 ◽

Vol 175 ◽

pp. 173-177 ◽

Cited By ~ 5

Author(s):

Yingchun Li ◽

Huihan Wang ◽

Rong Zhang ◽

Guojun Zhang ◽

Ying Yang ◽

...

Keyword(s):

Cell Lines ◽

Leukemia Cell ◽

In Vitro Cytotoxicity ◽

Human Leukemia ◽

Human Leukemia Cell ◽

Leukemia Cell Lines

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Apoptosis Induction in Human Leukemia Cell Lines by Gold Nanoparticles Synthesized Using the Green Biosynthetic Approach

Journal of Nanomaterials ◽

10.1155/2015/642621 ◽

2015 ◽

Vol 2015 ◽

pp. 1-10 ◽

Cited By ~ 10

Author(s):

Farideh Namvar ◽

Heshu Sulaiman Rahman ◽

Rosfarizan Mohamad ◽

Abdullah Rasedee ◽

Swee Keong Yeap ◽

...

Keyword(s):

Gold Nanoparticles ◽

Cell Lines ◽

Leukemia Cell ◽

Water Extract ◽

Human Leukemia ◽

Cytotoxicity Test ◽

Apoptosis Induction ◽

Human Leukemia Cell ◽

Leukemia Cell Lines

Gold nanoparticles were grown onSargassum muticumwater extract (S-GNPs) using the green biosynthetic approach. The nanoparticles were characterized using UV-visible spectroscopy, zeta potential, and transmission electron microscopy (TEM). The resulting S-GNPs were spherical and crystalline with a size of <10 nm. Thein vitroanticancer activity was demonstrated in human leukemia cell lines. The cancer cells were treated with different concentrations of S-GNPs, and calorimetric (MTT) assay used for the cytotoxicity test, which resulted in an IC50value of 4.22 ± 1.12, 5.71 ± 1.4, 6.55 ± 0.9, and 7.29 ± 1.7 μg/mL for each of the K562, HL-60, Jurkat, and CEM-ss cells, respectively. Thus, the K562 was selected for the next experiments. Furthermore, apoptosis induction was confirmed by Hoechst 33342, annexin V staining, and caspase-3/-9 activity tests. The cell cycle analysis exhibited a significant increase in the accumulation of S-GNPs treated cells at the sub-G1 phase, demonstrating the induction of apoptosis by S-GNPs. The nature of the inhibition of cancer cell growth by S-GNPs could open the way for further research in the design of green synthesis therapeutic agents, particularly in nanomedicine, for the treatment of cancer.

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