Abstract
An online computer program to monitor kinetic enzyme assays is described. The program analyzes the kinetic data in a manner similar to the way a technologist handles data in a manual procedure, taking into account the lag phase, substrate depletion phase, and linear portion of the rate curve. Thus, complete automation of even complex kinetic assays has been made practical. The program has been implemented for routine use in a clinical laboratory computer system ("LABCOM"), and the results correlate well with those obtained by established methods of manual data-handling procedures