scholarly journals Quantitative autoradiographic localization of prostaglandin E2 binding sites in monkey diencephalon

1988 ◽  
Vol 8 (6) ◽  
pp. 2003-2010 ◽  
Author(s):  
Y Watanabe ◽  
Y Watanabe ◽  
O Hayaishi
Keyword(s):  
Prostaglandin E2 ◽  
Binding Sites

Prostaglandin E2-binding sites and cAMP production in porcine fundic mucosa

1981 ◽  
Vol 241 (4) ◽  
pp. G313-G320
Author(s):  
B. L. Tepperman ◽  
B. D. Soper
Keyword(s):  
Adenylate Cyclase ◽  
Prostaglandin E2 ◽  
Binding Site ◽  
Binding Sites ◽  
Biologically Active ◽  
Cyclase Activity ◽  
Adenylate Cyclase Activity ◽  
Scatchard Analysis ◽  
Fundic Mucosa ◽  
Stimulation Of

Biologically active [3H]prostaglandin E2 (PGE2) bound rapidly and specifically to membrane fractions from hog fundic mucosa. Optimal binding occurred in the 30,000-g membrane preparation at 37 degrees C (pH 5.0). Scatchard analysis of specific PgE2 binding revealed the presence of a heterogeneous population of binding sites with Kd values and binding site concentrations of approximately 1 X 10(-9) M and 1 fmol/mg prot and 2 X 10(-8) M and 20 fmol/mg prot, respectively. Specific binding was inhibited by the following agents in descending order of potency: PGE1, PGA2, PGD2, 6-keto-PGF1 alpha, and thromboxane B2. Trypsin treatment or boiling reduced or abolished specific PGE2 binding. PGE2 stimulated cAMP formation in the 2,500-g fraction, with an approximate Km of 1 X 10(-6) M, but stimulation of adenylate cyclase activity by PG was not evident in the 16,000-g or 30,000-g tissue preparations. These results suggest that a specific PGE2-binding site exists in the 16,000-g and 30,000-g fractions of porcine fundic mucosa, although an increase in cAMP-forming capacity could not b of 1 X 10(-6) M, but stimulation of adenylate cyclase activity by PG was not evident in the 16,000-g or 30,000-g tissue preparations. These results suggest that a specific PGE2-binding site exists in the 16,000-g and 30,000-g fractions of porcine fundic mucosa, although an increase in cAMP-forming capacity could not b of 1 X 10(-6) M, but stimulation of adenylate cyclase activity by PG was not evident in the 16,000-g or 30,000-g tissue preparations. These results suggest that a specific PGE2-binding site exists in the 16,000-g and 30,000-g fractions of porcine fundic mucosa, although an increase in cAMP-forming capacity could not be localized in these fractions in vitro.

Subcellular distribution of [3H]-prostaglandin E2 binding sites in porcine gastric mucosa

1983 ◽  
Vol 25 (3) ◽  
pp. 425-441 ◽  
Author(s):  
Barry L. Tepperman ◽  
Brian D. Soper
Keyword(s):  
Gastric Mucosa ◽  
Prostaglandin E2 ◽  
Binding Sites ◽  
Subcellular Distribution

Detailed mapping of prostaglandin E2 binding sites in the brain

1991 ◽  
Vol 14 ◽  
pp. S141
Author(s):  
Kiyoshi Matsumura ◽  
Yumiko Watanabe ◽  
Hirotaka Onoe ◽  
Yoshimasa Koyama ◽  
Michael Connolly ◽  
...  
Keyword(s):  
Prostaglandin E2 ◽  
Binding Sites ◽  
The Brain

Modulation of 3H-prostaglandin E2 binding sites in the rabbit gastric mucosa

1990 ◽  
Vol 39 (2) ◽  
pp. 113-122 ◽  
Author(s):  
M. Tomoi ◽  
M. Matsuo ◽  
T. Ono ◽  
F. Shibayama
Keyword(s):  
Gastric Mucosa ◽  
Prostaglandin E2 ◽  
Binding Sites

High density of prostaglandin E2 (PGE2) binding sites in nucleus solitary tract (NST) and direct action of PGE2 on NST neurons

1991 ◽  
Vol 16 ◽  
pp. 42
Author(s):  
Kiyoshi Matsumura ◽  
Yumiko Watanabe ◽  
Hirotaka Onoe ◽  
Yasuyoshi Watanabe ◽  
Shinsuke Tanaka ◽  
...  
Keyword(s):  
Prostaglandin E2 ◽  
Binding Sites ◽  
Direct Action ◽  
High Density ◽  
Solitary Tract

Distribution of prostaglandin E2 binding sites in the porcine gastrointestinal tract

1981 ◽  
Vol 22 (2) ◽  
pp. 205-212 ◽  
Author(s):  
Barry L. Tepperman ◽  
Brian D. Soper
Keyword(s):  
Gastrointestinal Tract ◽  
Prostaglandin E2 ◽  
Binding Sites

scholarly journals Mapping the binding sites of UDP and prostaglandin E2 glyceryl ester in the nucleotide receptor P2Y6

ChemMedChem ◽  
2022 ◽  
Author(s):  
Anne Zimmermann ◽  
Oanh Vu ◽  
Antje Brüser ◽  
Gregory Sliwoski ◽  
Lawrence J. Marnett ◽  
...  
Keyword(s):  
Prostaglandin E2 ◽  
Binding Sites ◽  
Nucleotide Receptor
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