Phylogenetic Relationships of Neotomine-Peromyscine Rodents Using DNA Sequences from Beta Fibrinogen and Cytochrome BRelaciones FilogenÉTicas De Los Roedores Neotomino-Peromyscinos Utilizando Secuencias De Dos Loci Moleculares, El FibrinÓGeno Beta Y El Citocromo B

DNA barcoding has been recently promoted as a method for both assigning specimens to known species and for discovering new and cryptic species. Here we test both the potential and the limitations of DNA barcodes by analysing a group of well-studied organisms—the primates. Our results show that DNA barcodes provide enough information to efficiently identify and delineate primate species, but that they cannot reliably uncover many of the deeper phylogenetic relationships. Our conclusion is that these short DNA sequences do not contain enough information to build reliable molecular phylogenies or define new species, but that they can provide efficient sequence tags for assigning unknown specimens to known species. As such, DNA barcoding provides enormous potential for use in global biodiversity studies.Key words: DNA barcoding, species identification, primate, biodiversity.

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DNA BARCODING USING COI GENE SEQUENCES OF WILD BETTA FIGHTING FISH FROM INDONESIA: PHYLOGENY, STATUS AND DIVERSITY

Indonesian Fisheries Research Journal ◽

10.15578/ifrj.26.2.2020.97-105 ◽

2020 ◽

Vol 26 (2) ◽

pp. 97

Author(s):

Melta R. Fahmi ◽

Eni Kusrini ◽

Erma P. Hayuningtiyas ◽

Shofihar Sinansari ◽

Rudhy Gustiano

Keyword(s):

Genetic Diversity ◽

Genetic Distance ◽

Dna Sequences ◽

Phylogenetic Relationships ◽

Gene Sequence ◽

Ornamental Fish ◽

Coi Gene ◽

Tree Construction ◽

In The Wild ◽

Blast Program

The wild betta fish is a potential ornamental fish export commodity normally caught by traders or hobbyists in the wild. However, the population of wild betta in nature has declined and become a threat for their sustainability. This research was conducted to analyze the genetic diversity, phylogenetic relationships, and molecular identification through DNA COI gene sequence of Indonesian wild betta fish. A total of 92 wild betta fish specimens were collected in this study. Amplification of COI genes was carried out using Fish F1, Fish R1, Fish F2, and Fish R2 primers. The genetic diversity and phylogenetic relationships were analyzed using MEGA version 5 software program. Species identification of the specimen was conducted using BLAST program with 98-100% similarity value of the DNA sequences to indicate the same species. Phylogenetic tree construction showed seven monophyletic clades and showed that Betta smaragdina was the ancestral species of genus Betta in Indonesian waters. Genetic distance among species ranged from 0.02 to 0.30, whereas intra-species genetic distance ranged from 0 to 6.54.

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Phylogenetic relationships among the Caribbean members of the Cliona viridis complex (Porifera, Demospongiae, Hadromerida) using nuclear and mitochondrial DNA sequences

Molecular Phylogenetics and Evolution ◽

10.1016/j.ympev.2012.03.021 ◽

2012 ◽

Vol 64 (2) ◽

pp. 271-284 ◽

Cited By ~ 17

Author(s):

Dairo Escobar ◽

Sven Zea ◽

Juan A. Sánchez

Keyword(s):

Mitochondrial Dna ◽

Dna Sequences ◽

Phylogenetic Relationships ◽

The Caribbean

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