STUDIES ON THE GROWTH OF EXPERIMENTAL OVARIAN TUMOURS IN TISSUE CULTURE

1959 ◽  
Vol XXXII (I) ◽  
pp. 41-53 ◽  
Author(s):  
Stig Kullander ◽  
Bengt Källén
Keyword(s):  
Tissue Culture ◽  
Granulosa Cell ◽  
In Vitro Study ◽  
Vitro Study ◽  
Ovarian Tumours

ABSTRACT An in vitro study has been made of experimentally produced rat ovarian tumours of different age, paying particular attention to tumour reaction to crystallized steroids. Tumours of two histological structures were found: granulosa cell – luteoma tumours and arrhenoblastoma tumours. Both types grew in vitro and pictures of their cell appearance are given. The former type gave the best growth, and the endocrine studies were restricted to this type. The steroids tested (androsterone, oestrone, progesterone) all had an arresting effect in certain cases. This effect is not an unspecific, toxic one. The different tumours react to different extents, some being completely unaffected.

scholarly journals ANTIPLAQUE EFFICACY OF GANODERMA LUCIDUM TOOTHPASTE - AN IN VITRO STUDY

2018 ◽  
Vol 11 (11) ◽  
pp. 300
Author(s):  
Prabhjeet S ◽  
Meena A K ◽  
Jesil M
Keyword(s):  
Tissue Culture ◽  
Ganoderma Lucidum ◽  
In Vitro Study ◽  
Vitro Study ◽  
Distilled Water ◽  
Culture Plate ◽  
Significant Difference ◽  
Tissue Culture Plate

Objective: The objective of the study was to evaluate the efficacy of Ganoderma lucidum toothpaste as an antiplaque agent and to compare its efficacy with herbal toothpaste and mouthwash.Methods: Pooled saliva was collected in a sterile container from the volunteers after taking the consent. Tissue culture plate with 12 (3 × 4) wells was chosen. Pooled saliva of 20 mL was added to each well using the micropipette and was kept in the incubator at 37°C for 72 h. After 72 h, saliva was removed without touching the walls or the base of the wells. Each row was treated either with slurry prepared with Ganoderma/herbal/Colgate total toothpaste or herbal/chlorhexidine mouthwash/distilled water. One row of wells was kept as a control using erythrosine dye. After 30 s, all the wells were rinsed with distilled water. Erythrosine dye was added to all the wells, kept for 30 s, and rinsed with distilled water. The tissue culture plate was kept in the ELx800MS machine (ELISA reader) which was set at 540 nm, and the readings were obtained.Results: The results showed that G. lucidum toothpaste slurry reduced plaque than herbal and chlorhexidine mouthwash. However, there was no significant difference in plaque reduction between herbal and G. lucidum toothpaste slurries.Conclusion: The present study concluded that G. lucidum had better antiplaque efficacy than herbal toothpaste, herbal mouthwash, and chlorhexidine mouthwash.

scholarly journals In vitro study for stimulation effect of human bone marrow mesenchymal stem cell secretome on humen granulosa cell

2019 ◽  
Vol 112 (3) ◽  
pp. e420
Author(s):  
Hang-Soo Park ◽  
Abdeljabar El Andaloussi ◽  
Rishi Man Chugh ◽  
Amro Elsharoud ◽  
Mara Ulin ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Granulosa Cell ◽  
In Vitro Study ◽  
Human Bone ◽  
Vitro Study ◽  
Human Bone Marrow

scholarly journals Human tissue culture of osteochondral defect: An advanced in vitro model

2021 ◽  
Vol 4 (s1) ◽  
Author(s):  
Melania Maglio ◽  
Matilde Tschon ◽  
Stefania Pagani ◽  
Leonardo Vivarelli ◽  
Marco Govoni ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Human Tissue ◽  
Osteochondral Defect ◽  
In Vitro Study ◽  
In Vitro Models ◽  
Vitro Study ◽  
Alternative Methods ◽  
Wide Scope ◽  
Osteochondral Regeneration

The optimization of advanced in vitro models is essential for the development of alternative methods. The in vitro study of osteochondral regeneration still has numerous limitations and wide scope for exploration.

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