scholarly journals Physical and Linkage Maps for Drosophila serrata, a Model Species for Studies of Clinal Adaptation and Sexual Selection

2012 ◽  
Vol 2 (2) ◽  
pp. 287-297 ◽  
Author(s):  
Ann J. Stocker ◽  
Bosco B. Rusuwa ◽  
Mark J. Blacket ◽  
Francesca D. Frentiu ◽  
Mitchell Sullivan ◽  
...  
Keyword(s):  
Sexual Selection ◽  
Linkage Maps ◽  
Model Species ◽  
Drosophila Serrata

Integrating genomics and multivariate evolutionary quantitative genetics: a case study of constraints on sexual selection in Drosophila serrata

2021 ◽  
Vol 288 (1960) ◽  
Author(s):  
Adam J. Reddiex ◽  
Stephen F. Chenoweth
Keyword(s):  
Sexual Selection ◽  
Quantitative Genetics ◽  
Genome Wide Association Study ◽  
Mutational Bias ◽  
Stabilizing Selection ◽  
Genetic Constraints ◽  
Selection Gradients ◽  
Selection Responses ◽  
Drosophila Serrata ◽  
Potential Factors

In evolutionary quantitative genetics, the genetic variance–covariance matrix, G , and the vector of directional selection gradients, β , are key parameters for predicting multivariate selection responses and genetic constraints. Historically, investigations of G and β have not overlapped with those dissecting the genetic basis of quantitative traits. Thus, it remains unknown whether these parameters reflect pleiotropic effects at individual loci. Here, we integrate multivariate genome-wide association study (GWAS) with G and β estimation in a well-studied system of multivariate constraint: sexual selection on male cuticular hydrocarbons (CHCs) in Drosophila serrata . In a panel of wild-derived re-sequenced lines, we augment genome-based restricted maximum likelihood to estimate G alongside multivariate single nucleotide polymorphism (SNP) effects, detecting 532 significant associations from 1 652 276 SNPs. Constraint was evident, with β lying in a direction of G with low evolvability. Interestingly, minor frequency alleles typically increased male CHC-attractiveness suggesting opposing natural selection on β . SNP effects were significantly misaligned with the major eigenvector of G , g max , but well aligned to the second and third eigenvectors g 2 and g 3 . We discuss potential factors leading to these varied results including multivariate stabilizing selection and mutational bias. Our framework may be useful as researchers increasingly access genomic methods to study multivariate selection responses in wild populations.

scholarly journals SIGNAL TRAIT SEXUAL DIMORPHISM AND MUTUAL SEXUAL SELECTION IN DROSOPHILA SERRATA

Evolution ◽  
2003 ◽  
Vol 57 (10) ◽  
pp. 2326-2334 ◽  
Author(s):  
Stephen F. Chenoweth ◽  
Mark W. Blows
Keyword(s):  
Sexual Selection ◽  
Sexual Dimorphism ◽  
Drosophila Serrata

scholarly journals An expressed sequence tag (EST) library for Drosophila serrata, a model system for sexual selection and climatic adaptation studies

BMC Genomics ◽  
2009 ◽  
Vol 10 (1) ◽  
pp. 40 ◽  
Author(s):  
Francesca D Frentiu ◽  
Marcin Adamski ◽  
Elizabeth A McGraw ◽  
Mark W Blows ◽  
Stephen F Chenoweth
Keyword(s):  
Sexual Selection ◽  
Expressed Sequence Tag ◽  
Model System ◽  
Adaptation Studies ◽  
Climatic Adaptation ◽  
Est Library ◽  
Drosophila Serrata ◽  
Expressed Sequence

SIGNAL TRAIT SEXUAL DIMORPHISM AND MUTUAL SEXUAL SELECTION IN DROSOPHILA SERRATA

Evolution ◽  
2003 ◽  
Vol 57 (10) ◽  
pp. 2326 ◽  
Author(s):  
Stephen F. Chenoweth ◽  
Mark W. Blows
Keyword(s):  
Sexual Selection ◽  
Sexual Dimorphism ◽  
Drosophila Serrata

scholarly journals Faster rates of molecular sequence evolution in reproduction-related genes and in species with hypodermic sperm morphologies

2021 ◽  
Author(s):  
R Axel W Wiberg ◽  
Jeremias N Brand ◽  
Lukas Schaerer
Keyword(s):  
Sexual Selection ◽  
Sequence Evolution ◽  
Model Species ◽  
Free Living ◽  
Molecular Sequence ◽  
Functional Annotations ◽  
Genome Wide ◽  
Large Genus ◽  
Signatures Of Selection ◽  
Efficiency Of Selection

Sexual selection is expected to drive the evolution of many striking behaviours and morphologies, leaving signatures of selection at loci underlying these phenotypes. However, relatively few studies have contrasted molecular sequence evolution at such loci across lineages that differ in their sexual selection context. Our comparative genomics study of Macrostomum, a large genus of free-living simultaneously hermaphroditic flatworms, takes advantage of functional annotations from the model species, M. lignano, and transcriptome assemblies of 97 congeners. We compare molecular sequence evolution in species with contrasting sperm morphologies, which are strongly associated with multiple convergent shifts in the mating strategy and thus reflect the sexual selection context in Macrostomum. The sperm of most reciprocally mating species carry lateral bristles, likely functioning as anchoring mechanisms against post-copulatory sperm removal. Hypodermically mating species lack these bristles, potentially as adaptations to a different environment experienced by hypodermic sperm. We document faster molecular sequence evolution in reproduction-related, compared to ubiquitously-expressed, genes across all sperm morphologies, consistent with more intense selection acting on the former. Furthermore, we observed faster molecular sequence evolution in species with hypodermic sperm morphologies, in both reproduction-related and ubiquitously-expressed genes. These genome-wide patterns suggest that shifts to hypodermic mating reduce the efficiency of selection, possibly due to higher selfing rates in hypodermically mating species. Moreover, we find little evidence for convergent amino acid changes across species. We provide the first comprehensive comparative analysis of molecular sequence evolution in a group of simultaneously hermaphroditic animals, across well-replicated contrasts of lineages with divergent sperm morphologies.

scholarly journals Development of thirty-four new microsatellite loci and multiplexing of seven existing loci for Zootoca vivipara (Squamata: Lacertidae)

2017 ◽  
Vol 16 (1) ◽  
pp. 89 ◽  
Author(s):  
Jose Luis Horreo ◽  
Maria Luisa Peláez ◽  
Teresa Suárez ◽  
Benoit Heulin ◽  
Patrick Stefan Fitze
Keyword(s):  
Population Genetics ◽  
Population Dynamics ◽  
Sexual Selection ◽  
Microsatellite Loci ◽  
Population Studies ◽  
Effective Number ◽  
Model Species ◽  
Common Lizard ◽  
Zootoca Vivipara ◽  
Polymorphic Microsatellite

Few microsatellite loci exist for the European common lizard, Zootoca vivipara, a common model species in studies of population dynamics, sexual selection, population genetics, parity evolution, and physiology. The existing primers did not amplify in all lineages, and multiplexes were not optimized. A total of 34 new polymorphic microsatellite markers have been developed for this species and tested in 64 specimens belonging to oviparous and viviparous clades (B and D). The microsatellites were combined into seven different multiplexes. Results showed that all but one loci successfully amplified in all samples and both clades. The number of alleles detected per locus ranged 7–22 alleles and the effective number 1.58–7.82. The observed heterozygosity ranged 0.312–0.930, showing that all loci were highly variable. Oviparous and viviparous clades exhibited significant genetic differences (in FST). In addition to these new markers, the seven previously published and widely used microsatellite loci have been multiplexed and tested in oviparous clades. These innovations will allow for timesaving and robust analyses in Zootoca vivipara, boosting evolutionary and population studies and easing paternity analyses

No support for a meiosis suppressor in Daphnia pulex: Comparison of linkage maps reveals normal recombination in males of obligate parthenogenetic lineages.

2021 ◽  
Author(s):  
Cécile Molinier ◽  
Thomas Lenormand ◽  
Christoph R Haag
Keyword(s):  
Daphnia Pulex ◽  
Genetic Maps ◽  
Linkage Maps ◽  
Model Species ◽  
Recombination Rates ◽  
Entire Life ◽  
Entire Life Cycle ◽  
Genome Wide ◽  
Males And Females ◽  
Genomic Resource

It is often assumed that obligate parthenogenesis (OP) evolves by a disruption of meiosis and recombination. One emblematic example that appears to support this view is the crustacean Daphnia pulex. Here, by constructing high-density linkage maps, we estimate genome-wide recombination rates in males that are occasionally produced by OP lineages, as well as in males and females of cyclical parthenogenetic (CP) lineages. The results show no significant differences in recombination rates and patterns between CP and OP males nor between CP males and CP females. The observation that recombination is not suppressed in OP males invalidates the hypothesis of a general meiosis suppressor responsible for OP. Rather, our findings suggest that in D. pulex, as in other species where OP evolves from CP ancestors, the CP to OP transition evolves through a re-use of the parthenogenesis pathways already present in CP and through their extension to the entire life cycle, at least in females. In addition to the implications for the evolution of OP, the genetic maps produced by this study constitute an important genomic resource for the model species Daphnia.

scholarly journals Single-molecule sequencing of theDrosophila serratagenome

2016 ◽  
Author(s):  
Scott L. Allen ◽  
Emily K. Delaney ◽  
Artyom Kopp ◽  
Stephen F. Chenoweth
Keyword(s):  
Single Molecule ◽  
De Novo ◽  
Sequence Data ◽  
Error Rates ◽  
Model Species ◽  
High Coverage ◽  
Single Molecule Sequencing ◽  
Long Read ◽  
Drosophila Serrata ◽  
High Degree

ABSTRACTLong read sequencing technology promises to greatly enhancede novoassembly of genomes for non-model species. While error rates have been a large stumbling block, sequencing at high coverage allows reads to be self-corrected. Here we sequence andde novoassemble the genome ofDrosophila serrata, a non-model species from themontiumsubgroup that has been well studied for clines and sexual selection. Using 11 PacBio SMRT cells, we generated 12 Gbp of raw sequence data comprising approximately 65x whole genome coverage. Read lengths averaged 8,940 bp (NRead50 12,200) with the longest read at 53 Kbp. We self-corrected reads using the PBDagCon algorithm and assembled the genome using the MHAP algorithm within the PBcR assembler. Total genome length was 198 Mbp with an N50 just under 1 Mbp. Contigs displayed a high degree of arm-level conservation withD. melanogaster. We also provide an initial annotation for this genome usingin silicogene predictions that were supported by RNA-seq data.

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