Molecular characterization of a novel HMW glutenin subunit Dx2.3*tfromAegilops tauschii

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E. N. Yang ◽  
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Plants ◽  
2021 ◽  
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Zdenka Gálová ◽  
Svetlana Šliková ◽  
Leona Leišová-Svobodová ◽  
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A novel high molecular weight glutenin subunit encoded by the Glu-1B locus was identified in the French genotype Bagou, which we named 1B × 6.5. This subunit differed in SDS-PAGE from well-known 1B × 6 and 1B × 7 subunits, which are also encoded at this locus. Subunit 1B × 6.5 has a theoretical molecular weight of 88,322.83 Da, which is more mobile than 1B × 6 subunit, and isoelectric point (pI) of about 8.7, which is lower than that for 1B × 6 subunit. The specific primers were designed to amplify and sequence 2476 bp of the Glu-1B locus from genotype Bagou. A high level of similarity was found between the sequence encoding 1B × 6.5 and other x-type encoding alleles of this locus.


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