Development and Validation of a Short-Time Cell Culture and Multiplex Reverse Transcriptase Polymerase Chain Reaction Assay for Infectious Pancreatic Necrosis Virus in Mexican Farm-Sampled Rainbow Trout

2009 ◽  
Vol 21 (3) ◽  
pp. 167-172 ◽  
Author(s):  
Magda Barrera-Mejía ◽  
J. Simón-Martínez ◽  
Celene Salgado-Miranda ◽  
Fernando Vega ◽  
César Ortega ◽  
...  
Keyword(s):  
Cell Culture ◽  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Necrosis Virus ◽  
Chain Reaction ◽  
Infectious Pancreatic Necrosis ◽  
Polymerase Chain ◽  
Pancreatic Necrosis Virus ◽  
Short Time ◽  
Development And Validation

scholarly journals Effect of Quercetin on Infectious Pancreatic Necrosis Virus: in Vitro Replication Study

2020 ◽  
Vol 20 (2) ◽  
pp. 113-119
Author(s):  
Sermin Yildirim ◽  
Semra Gumusova
Keyword(s):  
Cell Culture ◽  
Rainbow Trout ◽  
Pancreatic Necrosis ◽  
Infectious Pancreatic Necrosis Virus ◽  
Cell Counting ◽  
Cytotoxicity Test ◽  
Viral Agent ◽  
Necrosis Virus ◽  
Infectious Pancreatic Necrosis ◽  
Pancreatic Necrosis Virus

Infectious Pancreatic Necrosis Virus (IPNV) is a viral agent that causes significant losses in salmonid fish in the rainbow trout and salmon farming sectors. The present study aimed to examine of quercetin effects on (IPNV) replication in Rainbow trout gonad (RTG-2) cell cultures. Half-maximal (50%) cytostatic concentration (CC 50) of quercetin were detected in the RTG-2 cell culture by cytotoxicity test with Cell Counting Kit-8 (CCK-8, Sigma-Aldrich) (n=3). Titration, cytopathogenic effect reduction, and qRT-PCR tests were used to determine the effect of quercetin on IPNV replication in RTG-2 cell culture. At the end of the cytotoxicity test, the non-toxic dose of quercetin was determined. Besides that, 50 μmol/L quercetin decreased the titer of IPNV in RTG-2 cells from 10-7 to 10-5, at the same time, the same dose of quercetin reduced the viral load to 40%. We concluded that quercetin is a potent antioxidant agent for IPNV infections and it can be an option in the treatment of the IPN disease.

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