scholarly journals Granulomatous pneumonia due to pythiosis in captive South American coatis (Nasua nasua)

2020 ◽  
Vol 40 (8) ◽  
pp. 647-650
Author(s):  
Kalinne S. Bezerra ◽  
Tarcísio A. Santos ◽  
Janaina M.A. Rosa ◽  
Carolina A. Pescador ◽  
Valeria Dutra ◽  
...  
Keyword(s):  
South American ◽  
Silver Stain ◽  
Pythium Insidiosum ◽  
Nasua Nasua ◽  
Methenamine Silver ◽  
Polymerase Chain ◽  
Methenamine Silver Stain ◽  
Clinical Records ◽  
Free Ranging ◽  
Granulomatous Pneumonia

ABSTRACT: Pythiosis is an emerging infectious disease affecting captive and free-ranging wild animals. We report granulomatous pneumonia due to Pythium insidiosum in two South American coatis (Nasua nasua), who were found dead without any clinical records. Severe granulomatous pneumonia associated with pleural effusion was revealed in the necropsy. Microscopically, variably sized granulomas and pyogranulomas presented negative hyphae profiles at the periphery of their necrotic cores. Grocott methenamine silver stain highlighted these structures, and immunostain (anti- P. insidiosum) was strongly positive. Molecular analysis by polymerase chain reaction amplified P. insidiosum specific DNA. These findings characterized P. insidiosum as a cause of granulomatous pneumonia in coatis and proved that pythiosis needs to be considered in the differential diagnosis of respiratory diseases affecting this species in endemic areas.

scholarly journals Equine cutaneous pythiosis: a report of four cases

2004 ◽  
Vol 34 (1) ◽  
pp. 289-292 ◽  
Author(s):  
Selwyn Arlington Headley ◽  
Homero Neves Arruda Junior
Keyword(s):  
Cutaneous Lesions ◽  
Silver Stain ◽  
Pythium Insidiosum ◽  
Methenamine Silver ◽  
Methenamine Silver Stain ◽  
Muscular Tissues

Cutaneous pythiosis is described in four horses in Northern Paraná, Brazil. All animals presented ulcerative, granulomatous, cutaneous lesions that did not invade adjacent muscular tissues. Histopathological evaluations revealed eosinophilic granulomatous reactions associated with intralesional hyphae suggestive of Pythium insidiosum observed at the margins of coagula. P. insidiosum hyphae were visualized by Gomori’s methenamine silver stain.

scholarly journals Canine Pythiosis - Isolation and Identification of Pythium Insidiosum

1989 ◽  
Vol 1 (4) ◽  
pp. 295-298 ◽  
Author(s):  
John Bentinck-Smith ◽  
Arvind A. Padhye ◽  
William R. Maslin ◽  
Carolyn Hamilton ◽  
Robert K. McDonald ◽  
...  
Keyword(s):  
Intestinal Tract ◽  
Subcutaneous Tissue ◽  
Isolation And Identification ◽  
Silver Stain ◽  
Pythium Insidiosum ◽  
Tissue Sections ◽  
Zoospore Formation ◽  
Methenamine Silver ◽  
Hematoxylin And Eosin ◽  
Methenamine Silver Stain

Pythium insidiosum was isolated from the subcutaneous tissue of a 1-year-old tan crossbreed dog and from the intestinal tract of an 18-month-old Samoyed male. Gomori's methenamine silver stain was superior to hematoxylin and eosin in demonstrating the organism in tissue sections. The agent was identified as P. insidiosum by zoospore formation in an aqueous yeast extract solution containing grass blades. Exoantigens produced in culture were shown to be identical to known P. insidiosum antigens by microimmunodiffusion.

Distinctive, rapid, and easy labeling of diffuse plaques in the Alzheimer brains by a new methenamine silver stain

1990 ◽  
Vol 79 (5) ◽  
pp. 569-572 ◽  
Author(s):  
H. Yamaguchi ◽  
C. Haga ◽  
S. Hirai ◽  
Y. Nakazato ◽  
K. Kosaka
Keyword(s):  
Silver Stain ◽  
Methenamine Silver ◽  
Methenamine Silver Stain ◽  
Diffuse Plaques

scholarly journals Pneumocystis jirovecii Pneumonia in a HIV-Infected Patient with a CD4 Count Greater Than 400 Cells/μL and Atovaquone Prophylaxis

2020 ◽  
Vol 2020 ◽  
pp. 1-4
Author(s):  
Abigayle Sullivan ◽  
Theresa Lanham ◽  
Ronald Krol ◽  
Shilla Zachariah
Keyword(s):  
Highly Active Antiretroviral Therapy ◽  
Lung Biopsy ◽  
Cd4 Count ◽  
Pneumocystis Jirovecii ◽  
Pneumocystis Jirovecii Pneumonia ◽  
Silver Stain ◽  
Highly Active ◽  
History Of ◽  
Methenamine Silver ◽  
Methenamine Silver Stain

We describe a rare case of Pneumocystis jirovecii pneumonia (PCP) in a heterosexual man with a pertinent medical history of well-controlled human immunodeficiency virus (HIV) on highly active antiretroviral therapy (HAART) and PCP prophylaxis with atovaquone. The patient presented with recurrent shortness of breath, worsening malaise, and fever, following treatment for hypersensitivity pneumonitis one month prior, including a twenty-four-day course of 40 milligrams daily glucocorticoid with taper. However, transbronchial biopsies, lavage, and cytology from prior admission were inconclusive. The patient refused video-assisted thoracic surgery (VATS) at that time. Upon readmission, bronchoscopy with right VATS and lung biopsy were performed. Grocott’s methenamine silver stain of right lung biopsy was positive for Pneumocystis jirovecii. This case is a rare example of PCP in a patient with a normal CD4 count (>487 cells/μL) and a low viral load (<20 copies/mL) despite PCP prophylactic antibiotics in the setting of recent iatrogenic immunosuppression.

Immunodetection of Pneumocystis carinii in bronchoalveolar lavage specimens compared with methenamine silver stain.

1996 ◽  
Vol 34 (3) ◽  
pp. 728-730 ◽  
Author(s):  
I Lautenschlager ◽  
O Lyytikainen ◽  
L Jokipii ◽  
A Jokipii ◽  
A Maiche ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage ◽  
Pneumocystis Carinii ◽  
Silver Stain ◽  
Methenamine Silver ◽  
Methenamine Silver Stain

Cytology of treated and minimalPneumocystis carinii pneumonia and a pitfall of the grocott methenamine silver stain

1991 ◽  
Vol 7 (1) ◽  
pp. 41-47 ◽  
Author(s):  
Sonya Naryshkin ◽  
Joan Daniels ◽  
Elaine Freno ◽  
Larry Cunningham
Keyword(s):  
Silver Stain ◽  
Methenamine Silver ◽  
Methenamine Silver Stain
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