Comparison of hemostatic efficacy of topical Ankaferd Blood Stopper on heparinized and nonheparinized rats in bleeding related to liver injury

Abstract Background: Several topical hemostats are available to help control surgical bleeding. Cutanplast is a highly absorbent and porous gelatin product that is available in Fast sponge and powder forms. This study investigated the hemostatic efficacy of Cutanplast Standard and Fast gelatin sponge and powder and Emosist oxidized regenerated cellulose (ORC) gauze in porcine liver and spleen surgical bleeding models.Methods: Cutanplast Standard and Fast gelatin sponge and Emosist ORC gauze were tested in liver abrasion/incision, liver puncture and spleen incision/puncture injuries, and Cutanplast Standard and Fast gelatin powder products were tested in liver abrasion/incision injuries. There were 13 liver injury (five abrasion, five incision and three puncture) and six spleen injury (three puncture and three incision sites) sites per animal.Results: Rapid hemostasis (≤ 2–5 min) was achieved in the liver abrasion and incision models with all Cutanplast gelatin sponge and powder products and Emosist ORC gauze, although in the liver incision model, time to hemostasis was > 5 min with Cutanplast Standard gelatin powder and Emosist ORC gauze. Rapid hemostasis occurred with Cutanplast Fast gelatin sponge and Emosist ORC gauze in the liver puncture and spleen puncture and incision models. In the spleen incision model, Cutanplast Standard gelatin sponge had a time to hemostasis approaching 10 min.Conclusion: Cutanplast gelatin sponge and powder products and Emosist ORC gauze may be suitable for surgical applications involving parenchymal organ bleeding, but certain products may perform better than others, including Cutanplast gelatin powder in diffuse mild bleeding (such as liver abrasion), and Cutanplast Fast gelatin sponge and Emosist ORC gauze for splenic bleeding.

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Hemostatic Efficacy and Biocompatibility Evaluation of a Novel Absorbable Porous Starch Hemostat

Surgical Innovation ◽

10.1177/15533506211046100 ◽

2021 ◽

pp. 155335062110461

Author(s):

Jianjun Zhu ◽

Zhuona Wu ◽

Wenzhong Sun ◽

Zhiyun Meng ◽

Xiaoxia Zhu ◽

...

Keyword(s):

Liver Injury ◽

Rat Liver ◽

Calcium Ion ◽

Blood Compatibility ◽

Potato Starch ◽

Control Group ◽

Tissue Compatibility ◽

Injury Model ◽

Hemostatic Efficacy

Background A novel absorbable porous starch hemostat (APSH) based on calcium ion-exchange crosslinked porous starch microparticles (Ca2+CPSM) was developed to improve hemostasis during surgeries for irregular cuts. The aim of this study was to compare its hemostatic efficacy and biocompatibility in a standard rat liver injury model relatively to Arista AH, Quickclean, and crosslinked porous starch microparticles (CPSM, without calcium ion). Methods 72 Wistar rats (220g–240 g) were randomly assigned to six groups (Arista, Quickclean, CPSM, Ca2+CPSM, native potato starch, and untreated control group, n =12 per group). 30 mg of each hemostatic agent was applied to a standard circular liver excision (8 mm in diameter and 3 mm deep) in rats. Following their hemostatic efficacy, in vivo biocompatiblity evaluation was examined. The native potato starch (NPS) group was used as the negative group. Results Ca2+CPSM had almost the same hemostatic efficacy compared with Arista; meanwhile, all the 4 hemostatic agents had good blood compatibility. In terms of in vivo tissue compatibility, Ca2+CPSM had relatively fast degradation and absorption rate with good histocompatibility. As the morphological, anatomic observation and H&E staining of liver defects after implantation, Ca2+CPSM was almost completely absorbed by liver tissue after 14 days. Conclusion According to our study, Ca2+CPSM could effectively achieve hemostasis in the standard rat liver injury model and exhibited good blood compatibility and in vivo tissue compatibility. These finding suggested that Ca2+CPSM as a new kind of APSH had its extensive clinical application value.

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Hemostatic Efficacy of Ankaferd Blood Stopper® in a Swine Bleeding Model

Medical Principles and Practice ◽

10.1159/000204344 ◽

2009 ◽

Vol 18 (3) ◽

pp. 165-169 ◽

Cited By ~ 41

Author(s):

Hasan Bilgili ◽

Ali Kosar ◽

Mevlut Kurt ◽

Ibrahim K. Onal ◽

Hakan Goker ◽

...

Keyword(s):

Ankaferd Blood Stopper ◽

Hemostatic Efficacy

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Comparison of the efficacy of five standard topical hemostats: a study in porcine liver and spleen models of surgical bleeding

10.21203/rs.3.rs-32186/v2 ◽

2020 ◽

Author(s):

Valerio Antonio Paternò ◽

Alessandro Bisin ◽

Alessandro Addis

Keyword(s):

Liver Injury ◽

Gelatin Sponge ◽

Regenerated Cellulose ◽

Oxidized Regenerated Cellulose ◽

Porcine Liver ◽

Surgical Bleeding ◽

Hemostatic Efficacy ◽

Better Than

Abstract Background: Several topical hemostats are available to help control surgical bleeding. Cutanplast is a highly absorbent and porous gelatin product that is available in Fast sponge and powder forms. This study investigated the hemostatic efficacy of Cutanplast Standard and Fast gelatin sponge and powder and Emosist oxidized regenerated cellulose (ORC) gauze in porcine liver and spleen surgical bleeding models.Methods: Cutanplast Standard and Fast gelatin sponge and Emosist ORC gauze were tested in liver abrasion/incision, liver puncture and spleen incision/puncture injuries, and Cutanplast Standard and Fast gelatin powder products were tested in liver abrasion/incision injuries. There were 13 liver injury (five abrasion, five incision and three puncture) and six spleen injury (three puncture and three incision sites) sites per animal.Results: Rapid hemostasis (≤ 2–5 min) was achieved in the liver abrasion and incision models with all Cutanplast gelatin sponge and powder products and Emosist ORC gauze, except in the liver incision model, time to hemostasis was > 5 min with Cutanplast Standard gelatin powder and Emosist ORC gauze. Rapid hemostasis occurred with Cutanplast Fast gelatin sponge and Emosist ORC gauze in the liver puncture and spleen puncture and incision models. In the spleen incision model, Cutanplast Standard gelatin sponge had a time to hemostasis approaching 10 min.Conclusion: Cutanplast gelatin sponge and powder products and Emosist ORC gauze may be suitable for surgical applications involving parenchymal organ bleeding, but certain products may perform better than others, including Cutanplast gelatin powder in diffuse mild bleeding (such as liver abrasion), and Cutanplast Fast gelatin sponge and Emosist ORC gauze for splenic bleeding.

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Hemostatic efficacy of human-like collagen sponge in arterioles and liver injury model

African Journal of Microbiology Research ◽

10.5897/ajmr12.102 ◽

2012 ◽

Vol 6 (10) ◽

Cited By ~ 1

Author(s):

Zhiguang Duan

Keyword(s):

Liver Injury ◽

Collagen Sponge ◽

Injury Model ◽

Hemostatic Efficacy

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Comparison of the efficacy of five standard topical hemostats: a study in porcine liver and spleen models of surgical bleeding

BMC Surgery ◽

10.1186/s12893-020-00874-w ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Valerio Antonio Paternò ◽

Alessandro Bisin ◽

Alessandro Addis

Keyword(s):

Liver Injury ◽

Gelatin Sponge ◽

Regenerated Cellulose ◽

Oxidized Regenerated Cellulose ◽

Porcine Liver ◽

Surgical Bleeding ◽

Hemostatic Efficacy ◽

Better Than

Abstract Background Several topical hemostats are available to help control surgical bleeding. Cutanplast is a highly absorbent and porous gelatin product that is available in Fast sponge and powder forms. This study investigated the hemostatic efficacy of Cutanplast Standard and Fast gelatin sponge and powder and Emosist oxidized regenerated cellulose (ORC) gauze in porcine liver and spleen surgical bleeding models. Methods Cutanplast Standard and Fast gelatin sponge and Emosist ORC gauze were tested in liver abrasion/incision, liver puncture and spleen incision/puncture injuries, and Cutanplast Standard and Fast gelatin powder products were tested in liver abrasion/incision injuries. There were 13 liver injury (five abrasion, five incision and three puncture) and six spleen injury (three puncture and three incision sites) sites per animal. Results Rapid hemostasis (≤ 2–5 min) was achieved in the liver abrasion and incision models with all Cutanplast gelatin sponge and powder products and Emosist ORC gauze, except in the liver incision model, time to hemostasis was > 5 min with Cutanplast Standard gelatin powder and Emosist ORC gauze. Rapid hemostasis occurred with Cutanplast Fast gelatin sponge and Emosist ORC gauze in the liver puncture and spleen puncture and incision models. In the spleen incision model, Cutanplast Standard gelatin sponge had a time to hemostasis approaching 10 min. Conclusion Cutanplast gelatin sponge and powder products and Emosist ORC gauze may be suitable for surgical applications involving parenchymal organ bleeding, but certain products may perform better than others, including Cutanplast gelatin powder in diffuse mild bleeding (such as liver abrasion), and Cutanplast Fast gelatin sponge and Emosist ORC gauze for splenic bleeding.

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