scholarly journals Genotyping, serotyping and determination of mating-type of Cryptococcus neoformans clinical isolates from São Paulo State, Brazil

Author(s):  
Marcelo Teruyuki Matsumoto ◽  
Ana Marisa Fusco-Almeida ◽  
Lilian Cristiane Baeza ◽  
Márcia de Souza Carvalho Melhem ◽  
Maria José Soares Medes-Giannini

The basidiomycetous yeast Cryptococcus neoformans is an important fungal pathogen mainly in immunocompromised patients. In this study, 47 clinical isolates of C. neoformans from regions of São Paulo State were studied serologically by using the Crypto Check Iatron RM 304-K kit, their genetic diversity was estimated by PCR-fingerprinting with a microsatellite-specific sequence (GACA)4, RAPD with primer 6 (Amersham Pharmacia Biotech), PCR-restriction fragment length polymorphism (RFLP) analysis of the phospholipase B gene (PLB1) digested with AvaI and mating type analysis by PCR. All 47 strains isolated from HIV positive patients included in this study were serotype A and MATalpha. The majority of the isolates (45/47) were VNI and only two were VNII by PCR-fingerprinting and PCR-RFLP analysis. High degree of homogeneity was observed when (GACA)4 was used, being highly correlated (> 0.9). In contrast, the RAPD analysis was more heterogeneous with higher number of molecular profiles. By PCR-RFLP, no new molecular type was found, enhancing the suggestion that the differences based on conserved gene as PLB1, can be resultant of ongoing divergent evolution within the C. neoformans complex, into the current eight subtypes. Our results furnish new information on the molecular epidemiology of C. neoformans in the southeast region of Brazil.

2007 ◽  
Vol 7 (1) ◽  
pp. 152-164 ◽  
Author(s):  
Ana Marisa Fusco Almeida ◽  
Marcelo Teruyuki Matsumoto ◽  
Lilian Cristiane Baeza ◽  
Rosana Bellan De Oliveira e Silva ◽  
Aline Aparecida Pizzirani Kleiner ◽  
...  

Author(s):  
D.K.B. Rodrigues ◽  
L.X. Bonfietti ◽  
R.A. Garcia ◽  
M.R. Araujo ◽  
J.S. Rodrigues ◽  
...  

2017 ◽  
Vol 165 (7-8) ◽  
pp. 414-424 ◽  
Author(s):  
Alfredo Seiiti Urashima ◽  
Augusto Freitas Alves ◽  
Flávio Nunes Silva ◽  
Danielle Oliveira ◽  
Rodrigo Gazaffi

2013 ◽  
Vol 14 ◽  
pp. 39-45 ◽  
Author(s):  
Maria Conceição Martins ◽  
Carmen M. Saraiva Giampaglia ◽  
Rosângela S. Oliveira ◽  
Vera Simonsen ◽  
Fábio Oliveira Latrilha ◽  
...  

Author(s):  
Liliane Maria Guimarães de Pinho ◽  
Vera Lúcia Garcia ◽  
Maria Cezira Fantini Nogueira-Martins

Implementation of a multiprofessional residency in family health in a city of São Paulo state: perception of the first residents (2014-2016)


2017 ◽  
Vol 314 (3) ◽  
pp. 1675-1681
Author(s):  
Elvis J. França ◽  
Elisabete A. De Nadai Fernandes ◽  
Felipe Y. Fonseca ◽  
Marcelo R. L. Magalhães ◽  
Mariana L. O. Santos

Author(s):  
Raquel Cardoso de Souza ◽  
Aline Andrade Godoy ◽  
Fábio Kummrow ◽  
Thyago Leandro dos Santos ◽  
Carlos Jesus Brandão ◽  
...  

2021 ◽  
Vol 141 ◽  
pp. 110805
Author(s):  
Giancarlo Aquila ◽  
Wilson Toshiro Nakamura ◽  
Paulo Rotella Junior ◽  
Luiz Celio Souza Rocha ◽  
Edson de Oliveira Pamplona

Author(s):  
Thaís Silvana de Carvalho ◽  
Marcelo Adorna Fernandes ◽  
Frésia Ricardi-Branco ◽  
Aline Marcele Ghilardi ◽  
Bernardo de Campos Pimenta e Marque Peixoto ◽  
...  

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