scholarly journals Effects of single and double infections with Potato virus X and Tobacco mosaic virus on disease development, plant growth, and virus accumulation in tomato

2002 ◽  
Vol 27 (3) ◽  
pp. 241-248 ◽  
Author(s):  
OLUSEGUN S. BALOGUN ◽  
LEIXIN XU ◽  
TOHRU TERAOKA ◽  
DAIJIRO HOSOKAWA
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Disease Severity ◽  
Potato Virus ◽  
Mixed Infection ◽  
Potato Virus X ◽  
Northern Hybridization ◽  
Post Inoculation ◽  
Virus Accumulation ◽  
Severity Of The Disease

The tomato cv. Fukuju nº. 2 was used for studying the effect of single and double infections with Potato virus X (PVX) and Tobacco mosaic virus (TMV). Mixed infection resulted in a synergistic increase of disease severity, where more growth reduction was seen with simultaneous inoculations than with sequential inoculations at four-day intervals. At five and 12 days post-inoculation, the increased severity of the disease coincided with enhancement of virus accumulation in the rapidly expanding upper leaves. The PVX concentration in leaves nº 5 to 7 of doubly infected plants was three to six fold that of singly infected ones, as determined by DAS-ELISA. Mixed infection with the L strain led to higher enhancement of PVX than with the TMV-L11A strain. The concentration of TMV-L was lower in double infection and significantly higher than TMV-L11A in both singly and doubly infected plants. Analyses of the PVX ORF2 by Western blot and Northern hybridization revealed the pattern of accumulation of the 25 kDa protein and the RNAs, respectively, following those of the virion and coat protein. The strain TMV-L11A overcame the resistance gene in cv. GCR 237 (Tm-1). In the upper leaf nº. 8, the concentration of PVX was three times higher in plants with mixed infection than with L11A. The concentrations of the L and OM (TMV strains) in both singly and doubly infected plants were at very low levels, and the synergistic effect on PVX concentration and disease severity was not observed.

scholarly journals Transgenic Plants Expressing Potato Virus X ORF2 Protein (p24) Are Resistant to Tobacco Mosaic Virus and Ob Tobamoviruses

1998 ◽  
Vol 72 (1) ◽  
pp. 731-738 ◽  
Author(s):  
X. Ares ◽  
G. Calamante ◽  
S. Cabral ◽  
J. Lodge ◽  
P. Hemenway ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Transgenic Tobacco ◽  
Potato Virus ◽  
Potato Virus X ◽  
N Gene ◽  
Transgenic Tobacco Plants ◽  
Tobacco Plants ◽  
Virus Accumulation

ABSTRACT The p24 protein, one of the three proteins implicated in local movement of potato virus X (PVX), was expressed in transgenic tobacco plants (Nicotiana tabacum Xanthi D8 NN). Plants with the highest level of p24 accumulation exhibited a stunted and slightly chlorotic phenotype. These transgenic plants facilitate the cell-to-cell movement of a mutant of PVX that contained a frameshift mutation in p24. Upon inoculation with tobacco mosaic virus (TMV), the size of necrotic local lesions was significantly smaller in p24+ plants than in nontransgenic, control plants. Systemic resistance to tobamoviruses was also evidenced after inoculation of p24+ plants with Ob, a virus that evades the hypersensitive response provided by the N gene. In the latter case, no systemic symptoms were observed, and virus accumulation remained low or undetectable by Western immunoblot analysis and back-inoculation assays. In contrast, no differences were observed in virus accumulation after inoculation with PVX, although more severe symptoms were evident on p24-expressing plants than on control plants. Similarly, infection assays conducted with potato virus Y showed no differences between control and transgenic plants. On the other hand, a considerable delay in virus accumulation and symptom development was observed when transgenic tobacco plants containing the movement protein (MP) of TMV were inoculated with PVX. Finally, a movement defective mutant of TMV was inoculated on p24+ plants or in mixed infections with PVX on nontransgenic plants. Both types of assays failed to produce TMV infections, implying that TMV MP is not interchangeable with the PVX MPs.

Effect of Kinetin on Tobacco Mosaic Virus and Potato Virus X Replication in Leaves of Systemic Hosts

1977 ◽  
Vol 90 (4) ◽  
pp. 342-349 ◽  
Author(s):  
A. V. Reunov ◽  
G. D. Reunova ◽  
L. A. Vasilyeva ◽  
V. G. Reifman
Keyword(s):  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Potato Virus ◽  
Potato Virus X

scholarly journals Tobacco mosaic virus (TMV) and potato virus X (PVX) coat proteins confer heterologous interference to PVX and TMV infection, respectively

2006 ◽  
Vol 87 (4) ◽  
pp. 1005-1012 ◽  
Author(s):  
A. A. Bazzini ◽  
S. Asurmendi ◽  
H. E. Hopp ◽  
R. N. Beachy
Keyword(s):  
Transgenic Plants ◽  
Tobacco Mosaic Virus ◽  
Mosaic Virus ◽  
Potato Virus ◽  
Quaternary Structure ◽  
Systemic Infection ◽  
Potato Virus X ◽  
Coat Proteins ◽  
Local Cell ◽  
Cell Spread

Replication of Potato virus X (PVX) was reduced in transgenic protoplasts that accumulated wild-type coat protein (CPWT) of Tobacco mosaic virus (TMV) or a mutant CP, CPT42W, that produced highly ordered states of aggregation, including pseudovirions. This reaction is referred to as heterologous CP-mediated resistance. However, protoplasts expressing a CP mutant that abolished aggregation and did not produce pseudovirions, CPT28W, did not reduce PVX replication. Similarly, in transgenic tobacco plants producing TMV CPWT or CPT42W, there was a delay in local cell-to-cell spread of PVX infection that was not observed in CPT28W plants or in non-transgenic plants. The results suggest that the quaternary structure of the TMV CP regulates the mechanism(s) of heterologous CP-mediated resistance. Similarly, transgenic protoplasts that produced PVX CP conferred transient protection against infection by TMV RNA. Transgenic plants that accumulated PVX CP reduced the cell-to-cell spread of infection and resulted in a delay in systemic infection following inoculation with TMV or TMV RNA. Heterologous CP-mediated resistance was characterized by a brief delay in systemic infection, whilst homologous CP-mediated resistance conferred reduced or no systemic infection.

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