scholarly journals Changes in peroxidase and polypeptide profiles in Nicotiana tabacum L. transformed with Agrobacterium rhizogenes

1995 ◽  
Vol 25 (2) ◽  
pp. 229-232
Author(s):  
Sergio Echeverrigaray

Morphological and proteic modifications in Nicotiana tabacum L. transformed by Agrobacterium rhizogenes were evaluated by the comparison of normal and transformant plants regenerated from hairy-roots formed by the strains A4 or IB-642 of A. rhizogenes. Changes in apical dominance were observed in IB-642 transformants, which exhibited an abnormal development of axiliary buds. The electrophoretic analysis indicated an increase in peroxidase activity and the induction of several isozymes of this complex in the transformants. The SDS-PAGE patterns comparison allows to identify several changes, specially, the increase in 31-33 and 54 kD polypeptides in the transformants.. Biochemical analysis suggests the induction of a pathogen or stress like response of the transformants due to the high auxin concentration codified by A. rhizogenes T-DNA incorporated to the plant genome.

2011 ◽  
Vol 30 (11) ◽  
pp. 2117-2129 ◽  
Author(s):  
Fengqing Wang ◽  
He Wei ◽  
Zhijun Tong ◽  
Xiaobo Zhang ◽  
Zemao Yang ◽  
...  

Author(s):  
Arne J. Aasen ◽  
Sven-Olof Almquist ◽  
Curt R. Enzell

Abstract35: two isomeric 5,6-Epoxy-3-hydroxy-7-megastigmen-9-ones from Nicotiana tabacum L.


Crop Science ◽  
1964 ◽  
Vol 4 (4) ◽  
pp. 349-353 ◽  
Author(s):  
T. J. Mann ◽  
J. A. Weybrew ◽  
D. F. Matzinger ◽  
J. L. Hall

Author(s):  
Monika Agacka-Mołdoch ◽  
Mian Abdur Rehman Arif ◽  
Ulrike Lohwasser ◽  
Teresa Doroszewska ◽  
Ramsey S. Lewis ◽  
...  

AbstractGenetic mapping of seed germination traits has been performed with many plant species. In tobacco, however, investigations are rare. In the present study, a bi-parental mapping population consisting of 118 doubled haploid lines and derived from a cross between ‘Beinhart-1000’ and ‘Hicks’ was investigated. Four germination-related traits, total germination (TG), normal germination (NG), time to reach 50% of total germination (T50), and the area under the curve after 200 h of germination (AUC) were considered by examining seeds either untreated or after a moderate controlled deterioration (CD). Quantitative trait loci were found for all traits distributed on 11 out of the 24 linkage groups. It was demonstrated that, as in many other species, germination-related traits are very complex and under polygenic control.


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