scholarly journals In vitro seed germination and seedling development of Annona crassiflora Mart.

2009 ◽  
Vol 66 (3) ◽  
pp. 410-413 ◽  
Author(s):  
Márcia de Nazaré Oliveira Ribeiro ◽  
Moacir Pasqual ◽  
Fabíola Villa ◽  
Leila Aparecida Salles Pio ◽  
Henk William Maria Hilhorst
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Gibberellic Acid ◽  
Seedling Development ◽  
Interactive Effects ◽  
Naphthalene Acetic Acid ◽  
Feasible Alternative ◽  
Annona Crassiflora ◽  
Short Time

Annona crassiflora Mart known as 'araticum', 'marolo' or 'field araticum' is a typical fruit from the Cerrado biome of Brazil with socio-economic and medicinal importance. Normally, Annona crassiflora is propagated through seeds. However, due to a deep dormancy that the seeds display at dispersion and the difficulty to obtain uniform plants in a short time period, micropropagation may be a feasible alternative. Concentrations of gibberellic acid (GA3) and naphthalene-acetic acid (NAA) and their interactive effects on in vitro seed germination and seedling development of Annona crassiflora were studied. Mature fruits of Annona crassiflora were depulped and the seeds washed in clear water and dried at room temperature. Seed coat was removed and the seeds were placed on Murashige & Skoog (MS) medium supplemented with gibberellic acid (GA3) and naphthalene-acetic acid (NAA), 30 g L-1 sucrose and 6 g L-1 agar-agar. Seeds were kept under these conditions for 30 days. After this period, seedlings were kept for another 90 days on Wood Plant Medium (WPM) with 20 g L-1 sucrose and 5 g L-1 agar-agar supplemented with the same GA3 and NAA concentrations. Cultures were incubated under controlled conditions at 25 ± 2°C temperature, 16: 8 (light: dark) photoperiod of 32 µmol m-2 s-1 irradiance provided by cool white fluorescent tubes (Philips). Use of WPM medium supplemented with 25-32 mg L-1 GA3 or MS with 26-30 mg L-1 GA3 and 2 mg L-1 NAA promoted rooting and plant growth.

scholarly journals In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume.

1970 ◽  
Vol 9 (9) ◽  
pp. 50-52 ◽  
Author(s):  
Bijaya Pant ◽  
Sumitra Shrestha ◽  
Shreeti Pradhan
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Seedling Development ◽  
Naphthalene Acetic Acid ◽  
Ideal Condition ◽  
Ms Medium ◽  
Mass Propagation ◽  
Asymbiotic Seed Germination ◽  
Protocorm Formation

In vitro seed germination and seedling development of Phaius tancarvilleae (L’Her.) Blume. was carried out on 0.8%(w/v) agar solidified MS Medium (Murashige and Skoog, 1962) without hormones or supplemented with different concentration and combination of Naphthalene acetic acid (NAA) and 6-benzylaminopurine (BAP). MS medium supplemented with 0.5 mg/l of BAP was the most ideal condition for early seed germination, protocorm formation and development of seedlings. Germination started after 7 weeks of culture and complete seedlings were obtained after 24 weeks of culture. This protocol might be helpful for mass propagation of orchids by asymbiotic seed germination. Keywords: Orchid; Invitro; Protocorm; Media DOI: http://dx.doi.org/10.3126/sw.v9i9.5518 SW 2011; 9(9): 50-52

scholarly journals In vitro propagation of the endangered orchid Dendrobium chryseum Rolfefrom protocorms culture

2020 ◽  
Vol 19 (1) ◽  
pp. 39-47 ◽  
Author(s):  
Sabitri Maharjan ◽  
Laxmi Sen Thakuri ◽  
Bir Bahadur Thapa ◽  
Shreeti Pradhan ◽  
Krishna K. Pant ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Gibberellic Acid ◽  
In Vitro Propagation ◽  
Seedling Development ◽  
Naphthalene Acetic Acid ◽  
Mass Propagation ◽  
Endangered Orchid ◽  
Immature Seeds ◽  
Indole 3 Acetic Acid

 The immature seeds of Dendrobiumchryseum, asympodial epiphytic orchid with yellow flowers, were cultured in vitro, and the resultant protocorms were used as explants for seedling development. Protocorms were cultured on½ M.S. medium fortified with Kinetin (Kn), 6-Benzylaminopurine (BAP), and Gibberellic Acid (GA3) in three concentrations (0.5mg/l, 1.0mg/land 2.0 mg/l) both alone and supplemented with 5% and 10% coconut water (C.W.). The highest number of shootsofD. chryseum developed on ½ - M.S. medium fortified with 2.0mg/lofKn and10% C.W. and the longest shootsdeveloped on ½ M.S. media fortified with 1.0mg/lGA3, and 10% C.W. The shoot derived from protocorms were placed in ½ M.S. medium fortified with three different rooting hormones, Indole -3- acetic acid (IAA), Indole -3-butyric acid (IBA) and α-Naphthalene acetic acid (NAA) in different concentrations alone as well as with each 1.0mg/l hormone combined with 10% C.W. The most effective of these media was ½ M.S. medium fortified with 1.5 mg/l IAA for rooting as well as for the production of longest roots. The present study could be useful for standardizing the protocol for mass propagation of the endangered orchid Dendrobiumchryseum.

scholarly journals Establishment of an EfficientIn VitroRegeneration Protocol for Rapid and Mass Propagation ofDendrobium chrysotoxumLindl. Using Seed Culture

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Potshangbam Nongdam ◽  
Leimapokpam Tikendra
Keyword(s):  
Acetic Acid ◽  
Seed Germination ◽  
Germination Percentage ◽  
Naphthalene Acetic Acid ◽  
Mass Propagation ◽  
Asymbiotic Seed Germination ◽  
Orchid Species ◽  
Seed Culture ◽  
Leaf Formation

An efficientin vitroregeneration protocol from seed culture has been established successfully forDendrobium chrysotoxum, an epiphytic orchid having tremendous ornamental and medicinal values. Seed germination response was encouraging in Mitra (M) medium enriched with different combinations of auxins and cytokinins. Medium supplemented with 0.4% activated charcoal (AC), 2 mg/L 6-benzyl amino purine (BAP), and 2 mg/L indole-3-acetic acid (IAA) produced best seed germination percentage in 2 weeks of culture. Incorporation of higher concentration of kinetin (KN) or BAP in combination with low auxin in medium induced pronounced shooting and leaf formation. Reduction in leaf development was evident when cytokinins exist singly in medium indicating synergistic effect of auxin and cytokinin in leaf induction. Presence of elevated level of indole-3-butyric acid (IBA) or 1-naphthalene acetic acid (NAA) with low cytokinin content in medium generated morein vitrorooting, though IBA was found to be more effective in rooting induction as compared to NAA. Thein vitroprotocol for asymbiotic seed germination developed from the present investigation can be used for rapid mass propagation of this highly importantDendrobiumorchid species.

In vitro Organogenesis in Cotton (Gossypium spp) for Ex-situ Conservation Issue

2021 ◽  
pp. 108-119
Author(s):  
Marius Guillaume Sinha ◽  
Gilles Habib Todjro Cacaï ◽  
Jerome Anani Houngue ◽  
Serge Sètondji Houédjissin ◽  
Thiéris Dassoudo Sossou ◽  
...  
Keyword(s):  
Seed Germination ◽  
Gibberellic Acid ◽  
Activated Charcoal ◽  
Genetic Erosion ◽  
Ex Situ ◽  
Naphthalene Acetic Acid ◽  
Zygotic Embryos ◽  
Local Varieties ◽  
Regeneration Rate

Background: Monovarietal cultivation of cotton plant allowed the genetic erosion for traditional cotton varieties that proceed essential traits mainly involved in cotton genetic breeding. These varieties need to be preserved for future used. This study aims to evaluate the effect of gibberellic acid on cotton seed germination and the effect of Benzylaminopurin (BAP), Kinetin (KIN), α-naphthalene acetic acid (NAA) and activated charcoal (CA) on cotton seedlings growth obtained from different type of explants. Methodology: The seeds of three improved varieties (KET782, ANG956, OKP768) and five local varieties (Q62, Q64, Q85, Q88, Q92) were germinated with different concentration of gibberellic acid (GA3). Different explants were collected from the germinated plantlets and cultivated in different culture media containing plants growth regulators and activated charcoal in different concentration. Results: We found that Gibberellic Acid activity on cotton seeds germination significantly varied according to the variety and GA3 concentration in the medium. 63.33% germination rate in OKP768 was obtained on the medium containing 0.5 mg/L of GA3 while the medium with 1 mg/l of GA3 gave the highest seed germination in Q85 (75%), Q64 (69.17%), and ANG956 (40.83%). The plantlets regeneration rate varied within the explants in different varieties used. 100%of axillary nodes were regenerated in Q62; Q85 and Q92 varieties while 60% were regenerated with the zygotic embryos in ANG956, and OKP768. Moreover, the medium supplemented with GA3 (1mg/l) + NAA (1 mg/l) induced the greatest number of roots (2.75 roots/plantlet) in the ANG956 and OKP768 varieties. With activated charcoal (10 g/l), ANG956 and OKP768 varieties achieved better performance with respective roots length average of 3.4 cm/plantlet and 2.1 cm/plantlet. The activated charcoal at 10 g/l highly influenced the length of roots with an average of 7.7cm in ANG956 variety. Implication: The protocol established during this study will be useful for in vitro regeneration and conservation for cotton local varieties.

scholarly journals In Vitro Adventitious Rooting of Carrizo Citrange Microshoots

HortScience ◽  
2010 ◽  
Vol 45 (6) ◽  
pp. 988-990 ◽  
Author(s):  
Almudena Montoliu ◽  
Aurelio Gómez-Cadenas ◽  
Rosa M. Pérez-Clemente
Keyword(s):  
Acetic Acid ◽  
Gibberellic Acid ◽  
Activated Charcoal ◽  
Culture Medium ◽  
Basal Medium ◽  
Poncirus Trifoliata ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Carrizo Citrange

The objective of this work was to develop an efficient in vitro rooting protocol for one of the most commercially used citrus rootstocks in Spain, Carrizo citrange (Citrus sinensis L. Osbeck × Poncirus trifoliata L. Raf.). Single-node cuttings taken from greenhouse-grown plants were cultured in petri dishes containing basal Murashige and Skoog medium. Shoots from nodal stem segments were excised and cultured in a multiplication medium (basal medium supplemented with 1.8 μM 6-benzylaminopurine) to promote the development of axillary buds. Individual shoots (15 mm long) were treated with different hormones at several concentrations for root induction evaluations. The addition of activated charcoal (AC) to the culture medium was also explored. The addition of auxins to the culture medium enhanced rooting percentage. Optimal results were obtained when 1-naphthalene acetic acid (10.8 μM) and gibberellic acid (0.3 μM) were added to the culture medium. The addition of AC to the rooting medium resulted in negative effects on the percentage of rooted shoots but had a positive effect on number of roots per rooted shoot. Chemical names used: activated charcoal (AC); 6-benzylaminopurine (BA); 1-naphthalene acetic acid (NAA); gibberellic acid (GA3); indole-3-butyric acid (IBA)

scholarly journals In Vitro Regeneration of Medicinally Important Shrub Carissa Opaca from Shoot Apices and Nodal Segments

2018 ◽  
Vol 3 ◽  
pp. 23-29
Author(s):  
Ali Ahmad ◽  
Bilal Haider Abbasi ◽  
Muhammad Zia
Keyword(s):  
Acetic Acid ◽  
Gibberellic Acid ◽  
Genetic Transformation ◽  
Nodal Segments ◽  
Naphthalene Acetic Acid ◽  
Ex Vitro ◽  
Shoot Apices ◽  
Murashige And Skoog Medium ◽  
Carissa Opaca

The study was aimed to develop efficient shoot regeneration fromex vitroexplants ofCarissa opaca, an imperative medicinal reservoir. Shoot apices and nodal segments were inoculated on MS (Murashige and Skoog) medium containing BAP (6-bezyl amino purine) and Kin (Kinetin) alone and in combination with NAA (naphthalene acetic acid) and GA3(Gibberellic acid). Higher concentrations of both cytokinins were found effective for regeneration from both explants. However, gibberellic acid and NAA addition with cytokinin, no persuading results were achieved. The shoot apices were found more effective inin vitroregeneration than nodal segments.The protocol can be effectively used for in vitro multiplication ofC. opaca, genetic transformation, and secondary metabolite production.

scholarly journals Effect of Different Media on in vitro Seed Germination and Seedling Development of Cymbidium aloifolium (L.) Sw.

2013 ◽  
Vol 14 (1) ◽  
pp. 51-56 ◽  
Author(s):  
Shreeti Pradhan ◽  
Tripti Regmi ◽  
Gaurav Parmar ◽  
Bijaya Pant
Keyword(s):  
Seed Germination ◽  
In Vitro Regeneration ◽  
Seedling Development ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Regeneration System ◽  
High Concentration ◽  
Plantlet Formation ◽  
Cymbidium Aloifolium

A comparative study on in vitro seed germination and seedling development of Cymbidium aloifolium (L.) Sw., an epiphytic medicinal orchid, was carried out on four different conditions of Murashige and Skoog (MS) and Knudson (KC) medium viz. full, ½. ¼ strength and medium supplemented with 0.5mg/l BAP (benzyl amino purine) and 0.5mg/l NAA (Naphthalene acetic acid). Varied response in terms of seed germination, protocorm formation and seedling development was observed on two different media. Medium supplemented with hormones favored optimum condition for the germination (approx. 90%) of seeds followed by full, ½ and ¼ strength on both MS & KC media. MS medium supplemented with 0.5mg/l BAP and 0.5mg/l NAA showed comparatively better response within 7 weeks of culture than other conditions of MS medium as well as KC medium. Based upon the results, it was found that MS medium was more effective than KC medium for germination, protocorm and plantlet formation. The present study has provided useful information that the high concentration of nutrient compounds supplemented with hormones are required for earlier in-vitro germination and plantlet development from immature seeds of C. aloifolium. It could be an important protocol to conserve this important orchid species by establishing an efficient in vitro regeneration system using immature seed culture. Nepal Journal of Science and Technology Vol. 14, No. 1 (2013) 51-56 DOI: http://dx.doi.org/10.3126/njst.v14i1.8878

scholarly journals Influence of growth regulators and explants on shoot regeneration in carnation

2009 ◽  
Vol 36 (No. 4) ◽  
pp. 140-146 ◽  
Author(s):  
J.K. Kanwar ◽  
S. Kumar
Keyword(s):  
Acetic Acid ◽  
Shoot Regeneration ◽  
Growth Regulators ◽  
Dianthus Caryophyllus ◽  
Naphthalene Acetic Acid ◽  
Ms Medium ◽  
Shoot Bud ◽  
Dichlorophenoxy Acetic Acid ◽  
Number Of Shoots

The influence of growth regulators, explants and their interactions on in vitro shoot bud formation from callus was studied in <I>Dianthus caryophyllus</I> L. The leaf and internode explants were cultured on Murashige and Skoog (MS) medium containing different concentrations of growth regulators. The highest callus induction was observed with 2 mg/l 2,4-dichlorophenoxy acetic acid (2,4-D) and 1 mg/l benzyl adenine (BA). Out of twenty seven shoot regeneration media tested, only 2 mg/l thidiazuron (TDZ) and zeatin alone or in combination with naphthalene acetic acid (NAA) and/or indole acetic acid (IAA) could differentiate calli. The highest average number of shoots was observed with 2 mg/l TDZ and 1 mg/l IAA. Significant differences were observed in calli producing shoots and number of shoots per callus in the explants of leaf and internode. The shoots were elongated and multiplied on MS medium supplemented with 1 mg/l BA and solidified with 1% agar. The shoots were rooted and hardened with 76% survival success in pots after six weeks of transfer to the pots.

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