scholarly journals Calyptospora sp. in Brachyplatystoma vaillantii trapped at the Vigia, State of Pará, Brazil

2012 ◽  
Vol 21 (2) ◽  
pp. 176-178 ◽  
Author(s):  
Moacir Cerqueira da Silva ◽  
Sérgio Carmona da São Clemente ◽  
José Antônio Picanço Júnior ◽  
Michele Velasco Oliveira da Silva
Keyword(s):  
Light Microscopy ◽  
Coastal Region ◽  
Differential Interference Contrast ◽  
First Occurrence ◽  
City State ◽  
Hepatic Coccidiosis

The article describes the first occurrence of hepatic coccidiosis in catfish of the species Brachyplatystoma vaillantii, captured in the coastal region of the Vigia city, state of Pará, Brazil, caused by species of the genus Calyptospora, family Calyptosporidae. Thirty specimens of piramutabas were examined where 60% were infected with liver location, featuring numerous mature and immature oocysts, grouped or isolated, with four sporocysts in pyriform shape. They were described on their morphology and dimensions of the oocysts and sporocysts, obtained from light microscopy and differential interference contrast.

scholarly journals Trichodina colisae (Ciliophora: Trichodinidae): new parasite records for two freshwater fish species farmed in Brazil

2012 ◽  
Vol 21 (4) ◽  
pp. 366-371 ◽  
Author(s):  
Gabriela Tomas Jerônimo ◽  
Natália da Costa Marchiori ◽  
Santiago Benites de Pádua ◽  
José Dias Neto ◽  
Fabiana Pilarski ◽  
...  
Keyword(s):  
South America ◽  
Light Microscopy ◽  
Silver Nitrate ◽  
Fish Species ◽  
Piaractus Mesopotamicus ◽  
The World ◽  
First Occurrence ◽  
Taxonomic Studies ◽  
Important Fish ◽  
Ciliate Protozoa

Family Trichodinidae comprises ciliate protozoa distributed worldwide; they are considered some of the main parasitological agents infecting cultivated fish. However, the trichodinidae parasitizing important fish species cultured in Brazil are unknown, and more taxonomic studies on this group of parasites are required. This research morphologically characterizes Trichodina colisae Asmat & Sultana, (2005) of pacu (Piaractus mesopotamicus) and patinga hybrid (P. mesopotamicus × P. brachypomus) cultivated in the central and southeast regions of the country. Fresh assemblies were made from mucus scraped from the skin, fins and gills, fixed with methanol and, subsequently, impregnated with silver nitrate and stained with Giemsa for assessment under light microscopy. This research reports not only the second occurrence of T. colisae in the world, but also its first occurrence in South America.

scholarly journals Detailed biostratigraphy of the Santonian/Campanian boundary interval in Northern Israel

1991 ◽  
Vol 10 (1) ◽  
pp. 39-50 ◽  
Author(s):  
A. Almogi-Labin ◽  
Y. Eshet ◽  
A. Flexer ◽  
A. Honigstein ◽  
S. Moshkovitz ◽  
...  
Keyword(s):  
Light Microscopy ◽  
Planktonic Foraminifera ◽  
Lower Boundary ◽  
Outer Shelf ◽  
Calcareous Nannoplankton ◽  
First Occurrence ◽  
Upper Slope

Abstract. One of the best continuous and fossiliferous Late Santonian-Early Campanian successions in Israel is the approximately 6m thick Kabri section in northern Israel. Its chalky marls were deposited in an outer shelf to upper slope environment with minor depth fluctuations. This Santonian/Campanian interval was studied examining calcareous nannoplankton, palynomorphs, ostracods and benthonic and planktonic foraminifera. The planktonic foraminiferal Dicarinella asymetrica - Globotruncanita elevata concurrent range zone was first observed in Israel in the Kabri section. The first occurrence of Aspidolithus parcus parcus herein is characterized by small specimens, difficult to determine by light microscopy. The lower boundary of the Campanian in this sequence was defined by the first occurrence of G. elevata, in accordance with the ammonite stage definition. This datum line nearly coincides with the first occurrence of the nannofossil marker A. parcus parcus and with the base of the Leguminocythereis dorsocostata (S-4) ostracod zone, both slightly above the foraminiferal boundary.

Cytoplasmic streaming and transport in the characean alga Nitella

1980 ◽  
Vol 58 (7) ◽  
pp. 786-796 ◽  
Author(s):  
N. Strömgren Allen
Keyword(s):  
Light Microscopy ◽  
Actin Filaments ◽  
Cytoplasmic Streaming ◽  
Three Dimensional ◽  
Differential Interference Contrast ◽  
Intercellular Transport ◽  
Cryoprotective Agents ◽  
Myosin Filaments ◽  
Microscopy Techniques

Plasmodesmata were recorded in vivo in Nitella furcata using polarization and differential interference-contrast light microscopy, techniques that could prove useful for further physiological experimentation on intercellular transport in characean cells.Freeze-fractured, deep-etch replicas of Nitella endoplasm were prepared without the use of cryoprotective agents. The endoplasm, in which rapid cytoplasmic streaming occurs, contains intricate three-dimensional networks of filaments of which two prominent size classes are characterized: 7- to 8-nm putative actin filaments and 4- to 5-nm putative myosin filaments. It is quite likely that the putative actin filaments are components of the endoplasmic filaments, and that these filaments interacting with the 4- to 5-nm filaments produce the motive force generating the observed cytoplasmic streaming.

Correlative Video-Enhanced Light Microscopy High-Resolution TEM

1988 ◽  
Vol 46 ◽  
pp. 124-125
Author(s):  
J. C. Lewis ◽  
N. L. Jones ◽  
N. S. Allen
Keyword(s):  
High Resolution ◽  
Light Microscopy ◽  
Spatial Data ◽  
Video Camera ◽  
Stereo Pair ◽  
Differential Interference Contrast ◽  
Differential Interference Contrast Microscopy ◽  
Normal Limits ◽  
Contrast Microscopy ◽  
Interference Contrast Microscopy

Allen video-enhanced contrast, differential interference-contrast microscopy (AVEC-DIC) combines high resolution differential interference contrast microscopy of the Zeiss IM35 microscope with a Hamamatsu video camera to obtain images with increased magnification, resolution, contrast and visibility. Through this combination and by capitalizing on light diffraction phenomena, it is possible to visualize subcellular organelles, particles, and structures which are an order of magnitude smaller (0.01 μm) than the normal limits of resolution with light microscopy (0.2 μm). The use of gold “finder” grids mounted on glass coverslips provides the mechanism by which cells after being observed in the video system can be located in the electron microscope. Such a correlative approach allows static ultrastructural features to be associated with cellular dynamics as observed and recorded by video. When the electron microscopy is done using either IVEM, HVEM or conventional TEM at 120 keV and the images recorded as stereo pair micrographs, the AVEC-DIC spatial data can be directly compared to high resolution 3-D data obtained by EM.

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