Taqman Real-Time PCR Assays for Rapid Detection of Avian PathogenicEscherichia coliIsolates

2014 ◽  
Vol 58 (4) ◽  
pp. 628-631 ◽  
Author(s):  
Nilo Ikuta ◽  
Fabiana de Oliveira Solla Sobral ◽  
Fernanda Kieling Moreira Lehmann ◽  
Vinicius Proença da Silveira ◽  
Silvia de Carli ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Pcr Assays

scholarly journals Rapid detection ofYersinia pestiswith multiplex real-time PCR assays using fluorescent hybridisation probes

2003 ◽  
Vol 38 (2) ◽  
pp. 117-126 ◽  
Author(s):  
Herbert Tomaso ◽  
Emil C Reisinger ◽  
Sascha Dahouk ◽  
Dimitrios Frangoulidis ◽  
Alexander Rakin ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Pcr Assays

scholarly journals Development of Candida-Specific Real-Time PCR Assays for the Detection and Identification of Eight Medically Important Candida Species

2016 ◽  
Vol 9 ◽  
pp. MBI.S38517 ◽  
Author(s):  
Jing Zhang ◽  
Guo-Chiuan Hung ◽  
Kenjiro Nagamine ◽  
Bingjie Li ◽  
Shien Tsai ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Candida Species ◽  
Rapid Detection ◽  
Corneal Transplantation ◽  
High Sensitivity ◽  
Turnaround Time ◽  
Pcr Assays ◽  
Primer Sets ◽  
Pcr Primer

Culture-based identification methods have been the gold standard for the diagnosis of fungal infection. Currently, molecular technologies such as real-time PCR assays with short turnaround time can provide desirable alternatives for the rapid detection of Candida microbes. However, most of the published PCR primer sets are not Candida specific and likely to amplify DNA from common environmental contaminants, such as Aspergillus microbes. In this study, we designed pan- Candida primer sets based on the ribosomal DNA-coding regions conserved within Candida but distinct from those of Aspergillus and Penicillium. We demonstrate that the final two selected pan- Candida primer sets would not amplify Aspergillus DNA and could be used to differentiate eight medically important Candida pathogens in real-time PCR assays based on their melting profiles, with a sensitivity of detection as low as 10 fg of Candida genomic DNA. Moreover, we further evaluated and selected species-specific primer sets covering Candida albicans, Candida glabrata, Candida tropicalis, and Candida dubliniensis and show that they had high sensitivity and specificity. These real-time PCR primer sets could potentially be assembled into a single PCR array for the rapid detection of Candida species in various clinical settings, such as corneal transplantation.

Real-time PCR assays for rapid detection ofZeugodacus cucumisandBactrocera jarvisi(Diptera: Tephritidae) for quarantine application

2018 ◽  
Vol 143 (1-2) ◽  
pp. 155-163 ◽  
Author(s):  
Dongmei Li ◽  
Sreelakshmi Nair ◽  
Diane Anderson ◽  
Prasad Doddala ◽  
Disna N. Gunawardana ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Pcr Assays

scholarly journals Development of Conventional and Real-Time PCR Assays for the Rapid Detection of Group B Streptococci

2000 ◽  
Vol 46 (3) ◽  
pp. 324-331 ◽  
Author(s):  
Danbing Ke ◽  
Christian Ménard ◽  
François J Picard ◽  
Maurice Boissinot ◽  
Marc Ouellette ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Culture Method ◽  
Conventional Pcr ◽  
Pcr Assay ◽  
Group B Streptococci ◽  
Standard Culture ◽  
Pcr Assays ◽  
Group B

Abstract Background: Group B streptococci (GBS), or Streptococcus agalactiae, are the leading bacterial cause of meningitis and bacterial sepsis in newborns. Currently available rapid methods to detect GBS from clinical specimens are unsuitable for replacement of culture methods, mainly because of their lack of sensitivity. Methods: We have developed a PCR-based assay for the rapid detection of GBS. The cfb gene encoding the Christie-Atkins-Munch-Petersen (CAMP) factor was selected as the genetic target for the assay. The PCR primers were initially tested by a conventional PCR method followed by gel electrophoresis. The assay was then adapted for use with the LightCyclerTM. For this purpose, two fluorogenic adjacent hybridization probes complementary to the GBS-specific amplicon were designed and tested. In addition, a rapid sample-processing protocol was evaluated by colony-forming unit counting and PCR. A total of 15 vaginal samples were tested by both standard culture method and the two PCR assays. Results: The conventional PCR assay was specific because it amplified only GBS DNA among 125 bacterial and fungal species tested, and was able to detect all 162 GBS isolates from various geographical areas. This PCR assay allowed detection of as few as one genome copy of GBS. The real-time PCR assay was comparable to conventional PCR assay in terms of sensitivity and specificity, but it was more rapid, requiring only ∼30 min for amplification and computer-based data analysis. The presence of vaginal specimens had no detrimental effect on the sensitivity of the PCR with the sample preparation protocol used. All four GBS-positive samples identified by the standard culture method were detected by the two PCR assays. Conclusion: These assays provide promising tools for the rapid detection and identification of GBS.

scholarly journals Development of Real-Time PCR Assays and Evaluation of Their Potential Use for Rapid Detection of Burkholderia pseudomallei in Clinical Blood Specimens

2007 ◽  
Vol 45 (9) ◽  
pp. 2894-2901 ◽  
Author(s):  
C. Supaprom ◽  
D. Wang ◽  
C. Leelayuwat ◽  
W. Thaewpia ◽  
W. Susaengrat ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Burkholderia Pseudomallei ◽  
Pcr Assays ◽  
Blood Specimens ◽  
Potential Use

scholarly journals Study of Real-Time PCR Assays for Rapid Detection of Food-Borne Pathogens

2005 ◽  
Vol 79 (9) ◽  
pp. 644-655 ◽  
Author(s):  
Hiroshi FUKUSHIMA ◽  
Yoshie TSUNOMORI
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Food Borne Pathogens ◽  
Food Borne ◽  
Pcr Assays

scholarly journals Development of Real-Time PCR Assays for Rapid Detection of Pfiesteria piscicida and Related Dinoflagellates

2002 ◽  
Vol 68 (6) ◽  
pp. 3180-3180 ◽  
Author(s):  
Holly A. Bowers ◽  
Torstein Tengs ◽  
Howard B. Glasgow ◽  
Joann M. Burkholder ◽  
Parke A. Rublee ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Pfiesteria Piscicida ◽  
Pcr Assays
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