A MEQ-Deleted Marek's Disease Virus Cloned as a Bacterial Artificial Chromosome is a Highly Efficacious Vaccine

2010 ◽  
Vol 5 (2) ◽  
pp. e16-e17
Author(s):  
Robert F. Silva ◽  
John R. Dunn ◽  
Hans H. Cheng ◽  
Masahiro Niikura
Keyword(s):  
Bacterial Artificial Chromosome ◽  
Disease Virus ◽  
Artificial Chromosome ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Efficacious Vaccine

scholarly journals Oncogenicity of Virulent Marek's Disease Virus Cloned as Bacterial Artificial Chromosomes

2004 ◽  
Vol 78 (23) ◽  
pp. 13376-13380 ◽  
Author(s):  
Lawrence Petherbridge ◽  
Andrew C. Brown ◽  
Susan J. Baigent ◽  
Ken Howes ◽  
Melanie A. Sacco ◽  
...  
Keyword(s):  
Disease Virus ◽  
Artificial Chromosome ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Wild Type Virus ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Bac Clone ◽  
Artificial Chromosomes ◽  
Bac Clones

ABSTRACT Marek's disease virus (MDV) is an oncogenic alphaherpesvirus that induces T-cell lymphomas in poultry. We report the construction of bacterial artificial chromosome (BAC) clones of the highly oncogenic RB-1B strain by inserting mini-F vector sequences into the US2 locus. MDV reconstituted from two BAC clones induced rapid-onset lymphomas similar to those induced by the wild-type virus. Virus reconstituted from another BAC clone that showed a 7.7-kbp deletion in the internal and terminal unique long repeat regions was nononcogenic, suggesting that the deleted region may be associated with oncogenicity. The generation of the oncogenic BAC clones of MDV is a significant step in unraveling the oncogenic determinants of this virus.

scholarly journals The products of the UL10 (gM) and the UL49.5 genes of Marek’s disease virus serotype 1 are essential for virus growth in cultured cells

2002 ◽  
Vol 83 (5) ◽  
pp. 997-1003 ◽  
Author(s):  
B. Karsten Tischer ◽  
Daniel Schumacher ◽  
Martin Messerle ◽  
Markus Wagner ◽  
Nikolaus Osterrieder
Keyword(s):  
Disease Virus ◽  
Cultured Cells ◽  
Artificial Chromosome ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Virus Growth ◽  
Virus Serotype ◽  
Serotype 1

The role of the products of the UL10 and the UL49.5 homologous genes of Marek’s disease virus serotype 1 (MDV-1) in virus replication was investigated. Deletion of either open reading frame in an infectious bacterial artificial chromosome clone (BAC20) of MDV-1 resulted in progeny viruses that were unable to spread from cell to cell. After transfection of UL10- or UL49.5-negative BAC20 DNA into chicken or quail cells, only single infected cells were observed by indirect immunofluorescence analysis. In contrast, plaque formation was restored when mutant BAC DNAs were co-transfected with the corresponding expression plasmid encoding either the UL10-encoded gM or the UL49.5 gene product. These data demonstrate that gM and its putative complex partner, the UL49.5 homologous protein, are essential for MDV-1 growth in cultured cells. Thus, MDV-1 represents the first example of a member of the family Herpesviridae for which the highly conserved membrane proteins are indispensable for cell-to-cell spread.

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