Four new genera and one new species of the subfamily Tarsosteninae (Coleoptera: Cleridae) are described. The new genera are:AgapetilusOpitz, gen. nov.,FallopylusOpitz, gen., nov,GloboclavaOpitz, gen. nov., andPseudopylusOpitz, gen. nov. The new species involvesAgapetilus vietusOpitz. sp. nov.LiostylusFairmaire is synonymized withRhophaloclerusFairmaire. New combinations,Fallopylus pallipes(MacLeay, 1872), comb. nov.,Globoclava quadrimaculata(Chevrolat, 1876), comb. nov.,Parapylus sedlaceki(Kolibáč, 2003), comb. nov.,Pseudopylus okei(Elston, 1929), comb. nov., andRhophaloclerus pictus(Fairmaire, 1902), comb. nov., are established. A key and phylogeny of the genera of Tarsosteninae is provided.