ABSENCE OF PYROGLUTAMYL-N3im-METHYL-HISTIDYL-PROLINEAMIDE (METHYL-THYROTROPHIN RELEASING HORMONE) IN THE RAT HYPOTHALAMUS

1978 ◽  
Vol 77 (3) ◽  
pp. 405-408 ◽  
Author(s):  
A. E. PEKARY ◽  
J. E. MORLEY ◽  
J. M. HERSHMAN
Keyword(s):  
Cation Exchange ◽  
Exchange Chromatography ◽  
Mammalian Brain ◽  
Synthetic Analogue ◽  
Cation Exchange Chromatography ◽  
Thyrotrophin Releasing Hormone ◽  
Releasing Hormone ◽  
Rat Hypothalamus

Pyroglutamyl-N3im-methyl-histidyl-prolineamide (methyl-thyrotrophin releasing hormone, methyl-TRH) is a potent synthetic analogue of TRH. N3im-Methyl-histidine is present in mammalian brain and it has been suggested that methyl-TRH is a physiological releasing hormone normally present in the hypothalamus. A non-gradient cation-exchange chromatography system that uses SP-Sephadex C-25 and completely resolves methyl-TRH and TRH has been developed. Because methyl-TRH cross-reacts in the immunoassay for TRH, this assay was used to measure TRH and methyl-TRH in the chromatographic fractions. By this means it has been demonstrated that the amount of methyl-TRH present in the rat is less than 0·025 ng/hypothalamus.

Thyrotrophin releasing hormone and related peptides in canine tissues

1983 ◽  
Vol 98 (3) ◽  
pp. 299-306 ◽  
Author(s):  
A. E. Pekary ◽  
Melvyn Richkind ◽  
J. M. Hershman
Keyword(s):  
Total Concentration ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography ◽  
Thyrotrophin Releasing Hormone ◽  
Releasing Hormone ◽  
Cation Exchangers ◽  
Binding Substance ◽  
Exclusion Chromatography ◽  
Liver Homogenates ◽  
Dog Liver

Thyrotrophin releasing hormone (TRH)-immunoreactive peptides have been quantified in canine serum, hypothalamus, liver, pancreas, adrenal, thyroid, prostate, testis, epididymis and semen by TRH radioimmunoassay, SP-Sephadex C-25 cation exchange chromatography, Sephadex G-10 exclusion chromatography and high pressure liquid chromatography. The total concentration of TRH and TRH-like peptides was highest in the hypothalamus, followed by liver, adrenal, pancreas, thyroid, prostate, epididymis, testis and serum. All of the TRH immunoreactivity (TRH-IR) within extracts of the hypothalamus was due to TRH. On the other hand, nearly all of the TRH-IR of extracts of liver, thyroid, prostate, epididymis, testis and semen was due to TRH-homologous peptides. Adrenal and pancreatic extracts contained a greater proportion of TRH in relation to the TRH-homologous peptides. Extracts of dog serum and semen were found to contain a TRH-binding substance which reduced the retention of added TRH by cation exchangers. The half-time of disappearance (t½) of synthetic TRH incubated at 23 °C in 10% (w/v) homogenates in 0·15 m-NaCl–0·05 m-phosphate buffer, pH 7·5, ranged from 22±10 (s.d.) min for liver to 120 ±58 min for thyroid. The short t½ for TRH added to dog liver homogenates contrasted with a previous report that dog liver is essentially free of TRH-degrading activity.

Strong cation-exchange chromatography of proteins on a sulfoalkylated monolithic cryogel

2015 ◽  
Vol 1386 ◽  
pp. 13-21 ◽  
Author(s):  
Işık Perçin ◽  
Rushd Khalaf ◽  
Bastian Brand ◽  
Massimo Morbidelli ◽  
Orhan Gezici
Keyword(s):  
Cation Exchange ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography ◽  
Strong Cation Exchange ◽  
Strong Cation Exchange Chromatography

Structural characteristics of low-molecular-mass displacers for cation-exchange chromatography

1998 ◽  
Vol 814 (1-2) ◽  
pp. 83-95 ◽  
Author(s):  
Abhinav A Shukla ◽  
Kristopher A Barnthouse ◽  
Sung Su Bae ◽  
J.A Moore ◽  
Steven M Cramer
Keyword(s):  
Cation Exchange ◽  
Molecular Mass ◽  
Structural Characteristics ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography

Characterization of the Acidic Species of a Monoclonal Antibody Using Weak Cation Exchange Chromatography and LC-MS

2015 ◽  
Vol 87 (17) ◽  
pp. 9084-9092 ◽  
Author(s):  
Gomathinayagam Ponniah ◽  
Adriana Kita ◽  
Christine Nowak ◽  
Alyssa Neill ◽  
Yekaterina Kori ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Cation Exchange ◽  
Exchange Chromatography ◽  
Cation Exchange Chromatography ◽  
Weak Cation Exchange
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