Enhancement of bovine growth hormone activity by antibodies against growth hormone peptides

1990 ◽  
Vol 125 (1) ◽  
pp. 31-38 ◽  
Author(s):  
R. Bomford ◽  
R. Aston
Keyword(s):  
Growth Hormone ◽  
Solid Phase ◽  
Growth Promotion ◽  
Costal Cartilage ◽  
Growth Hormones ◽  
Peptide Sequence ◽  
Hormone Activity ◽  
High Affinity ◽  
Solid Phase Radioimmunoassay ◽  
Dwarf Mice

ABSTRACT The biological activity of growth hormones can be enhanced by complexing with monoclonal antibodies of appropriate specificity. In order to define the regions associated with the phenomenon, site-directed antisera to ovine GH (oGH) were prepared by vaccination of sheep with synthetic peptides. Peptides from six distinct regions of the oGH molecule raised antibodies which recognized the hormone in solid-phase radioimmunoassay; however, only one peptide elicited high-affinity antibody as determined by liquid-phase assay. This peptide, corresponding to amino acid sequence 35–53, resulted in circulating hormone antibody in the majority of vaccinated sheep. Immunoglobulin prepared from the serum of immunized animals produced an enhancement of the somatotrophic activity of exogenously administered GH in dwarf mice as determined by the incorporation of [35S]sulphate into costal cartilage. The identification of an antigenic peptide sequence from oGH/bovine GH which elicits enhancing antisera, raises the possibility of a growth-promotion vaccine. Journal of Endocrinology (1990) 125, 31–38

GROWTH HORMONE AND RADIO-SULFATE INCORPORATION IN COSTAL CARTILAGE

1961 ◽  
Vol 37 (2) ◽  
pp. 176-182 ◽  
Author(s):  
Elliott J. Collins ◽  
Vernon F. Baker
Keyword(s):  
Growth Hormone ◽  
Costal Cartilage ◽  
Time Response ◽  
Hormone Activity ◽  
Reliable Estimation ◽  
Hypophysectomized Rats ◽  
Related Response ◽  
Sulfate Incorporation

ABSTRACT The characteristics and nature of the effect of growth hormone on the incorporation of radio-sulfate into the costal cartilage of hypophysectomized rats has been studied. The time-response studies indicate that a reliable estimation of growth hormone activity can be ascertained within a 24 hour period, and a reproducible dose-related response can be obtained at dosage levels ranging from 12-48 μg. Growth hormone stimulates the synthesis of organic sulfates and accumulation of inorganic sulfates within 48 hours.

EFFECTS OF GROWTH HORMONE, PROLACTIN AND THYROXINE ON BODY WEIGHT, SOMATOMEDIN-LIKE ACTIVITY AND IN-VIVO SULPHATION OF CARTILAGE IN HYPOPITUITARY DWARF MICE

1980 ◽  
Vol 85 (1) ◽  
pp. 35-47 ◽  
Author(s):  
A. T. HOLDER ◽  
M. WALLIS ◽  
P. BIGGS ◽  
M. A. PREECE
Keyword(s):  
Growth Hormone ◽  
Costal Cartilage ◽  
Tail Length ◽  
Bovine Growth Hormone ◽  
Dwarf Mouse ◽  
Dwarf Mice ◽  
Dose Dependent Increase ◽  
Dose Dependent ◽  
In Vivo Uptake

SUMMARY Hypopituitary dwarf mice were found to have reduced levels of serum somatomedin-like activity compared with normal mice of the Snell strain. Treatment with bovine growth hormone for 3 and 7 days resulted in growth without significantly increased levels of serum somatomedin-like activity, as detected by in-vitro uptake of 35SO42− into normal rat cartilage; only after treatment for 14 days was somatomedin activity significantly raised. However, treatment for 2 days with bovine growth hormone, bovine prolactin or thyroxine resulted in a dose-dependent increase in in-vivo uptake of 35SO42− into dwarf mouse costal cartilage; growth hormone and thyroxine did not act synergistically. Ten days of treatment with growth hormone promoted a dose-dependent increase in both growth (increased weight gain and tail length) and in-vivo uptake of 35SO42−. Increase in tail length was correlated with uptake of 35SO42−. Thus, in-vivo uptake of 35SO42− into dwarf mouse costal cartilage provides a sensitive method for detecting a dose-related effect of growth hormone.

THE BIOASSAY OF GROWTH HORMONE IN SNELL'S DWARF MICE: EFFECTS OF THYROXINE AND PROLACTIN ON THE DOSE—RESPONSE CURVE

1973 ◽  
Vol 56 (2) ◽  
pp. 235-243 ◽  
Author(s):  
M. WALLIS ◽  
JENNIFER A. DEW
Keyword(s):  
Growth Hormone ◽  
Response Curve ◽  
Growth Response ◽  
Growth Promotion ◽  
Promoting Effect ◽  
Pituitary Growth Hormone ◽  
Growth Promoting ◽  
Dependent Growth ◽  
Dwarf Mice ◽  
Dose Dependent

SUMMARY Pituitary growth hormone has a dose-dependent growth promoting effect in pituitary dwarf mice (Snell's strain), and this effect can be used as the basis of a bioassay for the hormone. Prolactin and thyroxine also promote growth in these animals, and the effects of these hormones in combination with growth hormone were studied, in order to see whether their presence might enhance the precision or sensitivity of the growth hormone assay. When prolactin and/or thyroxine were administered with growth hormone, the growth response observed was no greater than the sum of the effects of the hormones given separately; in some cases it was less. Neither prolactin nor thyroxine increase the sensitivity or precision of the growth hormone bioassay. The implications of these results for theories about the mechanisms of growth promotion by these hormones are considered.

Solid-phase radioimmunoassay for human growth hormone

1969 ◽  
Vol 6 (5) ◽  
pp. 699-713 ◽  
Author(s):  
J.B. Baumann ◽  
J. Girard ◽  
M. Vest
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Solid Phase ◽  
Human Growth ◽  
Solid Phase Radioimmunoassay

Concentration of free growth hormone-binding protein in the serum of mice is not regulated by growth hormone

1997 ◽  
Vol 153 (2) ◽  
pp. 319-325 ◽  
Author(s):  
A I Sotelo ◽  
F P Dominici ◽  
A Bartke ◽  
D Turyn
Keyword(s):  
Growth Hormone ◽  
Binding Protein ◽  
High Capacity ◽  
Peripheral Circulation ◽  
Mouse Serum ◽  
High Affinity ◽  
Ames Dwarf Mice ◽  
Ames Dwarf ◽  
Hormone Binding Protein ◽  
Dwarf Mice

Abstract Ames dwarf mice that do not express growth hormone (GH) or prolactin (PRL) genes were used to study the effects of GH deficiency on the presence and the characteristics of GH-binding protein (GHBP) in serum. Chromatographic techniques were used to allow characterization of biological rather than immunological activity of GHBP. Two GH-binding fractions were found in dwarf mice serum, one with low affinity and high capacity (GHBPI) and one with high affinity, low capacity and lower molecular mass (GHBPII). Serum concentration of the high-affinity GHBP was 0·73 ± 0·03 nm with a Kd of 6·3 ± 1·7 nm. Since Ames dwarf mice have no GH in the circulation, all the GHBP is free. Interestingly, the concentration of GHBP in dwarf mice was similar to the levels of free GHBP measured in normal mice from the same line. Moreover, this value (0·7 nm) closely resembles the concentration of free GHBP in the serum of transgenic mice overexpressing GH, in which peripheral GH levels are grossly elevated. These observations can be interpreted as evidence that the levels of free GHBP in mouse serum are independent of GH concentration, and that GH influences only the levels of bound GHBP in peripheral circulation. Journal of Endocrinology (1997) 153, 319–325

scholarly journals Solid-Phase Radioimmunoassay of Human Growth Hormone

1966 ◽  
Vol 100 (3) ◽  
pp. 31C-33C ◽  
Author(s):  
Kevin Catt ◽  
H. D. Niall ◽  
G. W. Tregear
Keyword(s):  
Growth Hormone ◽  
Human Growth Hormone ◽  
Solid Phase ◽  
Human Growth ◽  
Solid Phase Radioimmunoassay

Radioimmunoassay of Fibrinogen and Its Proteolysis Products

1968 ◽  
Vol 20 (01/02) ◽  
pp. 001-006 ◽  
Author(s):  
K. J Catt ◽  
J Hirsh ◽  
D. J Castelan ◽  
H. D Niall ◽  
G. W Tregear
Keyword(s):  
Solid Phase ◽  
Solid Phase Radioimmunoassay ◽  
Radioimmunoassay Method

SummaryThe solid-phase radioimmunoassay method has been applied to the measurement of fibrinogen. The method is extremely sensitive, being able to detect fibrinogen concentrations as low as 10 ng/ml. The immunoreactivity of fibrinogen proteolysis products differs from that of native fibrinogen, early proteolysis products showing enhanced immunoreactivity which decreases progressively with further digestion.

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