scholarly journals Influence of calpain on lamb myofibrillar proteins degradation during in vitro culture

2018 ◽  
Vol 36 (No. 4) ◽  
pp. 296-300
Author(s):  
Muhtar Mukaddas ◽  
Qiao Ma Yi ◽  
Ablikim Batur ◽  
Abduwali Arzugul ◽  
Sulayman Yusup
Keyword(s):  
In Vitro Culture ◽  
Meat Quality ◽  
Troponin T ◽  
Culture Method ◽  
Myofibrillar Protein ◽  
Myofibrillar Proteins ◽  
Important Indicator ◽  
Quality Degradation ◽  
Sds Page

Tenderness is an important indicator of meat quality, degradation plays important role during the maturation, therefore improving meat quality and tenderness. Understanding the process of myofibrillar and which enzymes affect degradation is more needed to prove. In this study we used longest muscle of Bashibai sheep of Xinjiang as the experiment material. The isolated myofibrillar protein and μ-calpains are incubated at 4°C about 1, 3, 7, 14, and 21 days using in vitro culture method and analysed by SDS-PAGE, Western blotting. Results showed that μ-calpain can improve the degradation of some myofibrillar proteins. Desmin was degraded by the 3<sup>rd</sup>, troponin–T was completely degraded by the 7<sup>th</sup> day.

scholarly journals Effect of Ultimate pH on Postmortem Myofibrillar Protein Degradation and Meat Quality Characteristics of Chinese Yellow Crossbreed Cattle

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Peng Li ◽  
Tiantian Wang ◽  
Yanwei Mao ◽  
Yimin Zhang ◽  
Lebao Niu ◽  
...  
Keyword(s):  
Enzymatic Activity ◽  
Protein Degradation ◽  
Meat Quality ◽  
Troponin T ◽  
Quality Characteristics ◽  
Myofibrillar Protein ◽  
Cooking Loss ◽  
Crossbred Cattle ◽  
Beef Tenderness ◽  
Fragmentation Index

This paper describes the complex effects of postmortem ultimate pH (pHu) on Chinese Yellow crossbreed cattle quality during postmortem ageing and provides an explanation of how pHu affects beef tenderness. High pHu beef had the highest initial tenderness (P<0.05) compared with other groups at 1 day postmortem. Intermediate and low pHu beef had similar initial WBSF at 1 day postmortem, but intermediate pHu beef had slower tenderization rate than low pHu beef (P<0.05). Purge loss, cooking loss,L*,a*, andb*values decreased with increasing pHu during ageing (P<0.05). Myofibril fragmentation index (MFI) was higher in high pHu beef than intermediate and low pHu beef throughout ageing (P<0.05). Protein degradation studies found that desmin and troponin-T appeared degraded within 0.5 h postmortem for high and low pHu beef, compared to >2 days for intermediate pHu beef. Overall, Chinese Yellow crossbred cattle tenderness is related to pHu, which may be affected by proteolytic enzymatic activity. Therefore, pHu may be used to predict beef tenderness and other quality characteristics during postmortem ageing. To achieve consistent tenderness, different ageing times should be used, depending on pHu.

An ultrastructural study of embryo and endosperm development during in vitro culture of maize ovaries (Zea mays)

1986 ◽  
Vol 64 (10) ◽  
pp. 2227-2238 ◽  
Author(s):  
J. H. N. Schel ◽  
H. Kieft
Keyword(s):  
In Vitro Culture ◽  
Embryo Development ◽  
Light And Electron Microscopy ◽  
Culture Method ◽  
Endosperm Development ◽  
Endosperm Formation ◽  
Development In Vitro ◽  
Maize Embryos

A culture method is described which allows the continuous supply of fresh liquid medium and which prevents the accumulation of toxic metabolites. Development of maize embryos and endosperm after various periods of in vitro ovary culture was studied by light and electron microscopy. Using this method the ultrastructural features of embryo development in vitro were similar to those of in vivo embryos. In contrast, the formation of endosperm was irregular with the absence of cellularization of the inner endosperm being frequent. In some cases, only the endosperm developed without any indication of embryo formation. In a calcium-depleted medium, embryo development was normal but again, endosperm formation was aberrant. No cells were formed in the central part of the endosperm and near the placental region degeneration took place, resulting in vacuoles with dark inclusions, clumps of rough endoplasmic reticulum membranes, and cellular breakdown. The events occurring after in vitro culture strongly resemble those taking place after intergeneric crosses or crosses between diploid and tetraploid strains. It is concluded that defective endosperm development is probably the main factor for the failure of embryo development.

scholarly journals Comparative suitability of two culture media to the in vitro growth of embryos of three coconut types

2007 ◽  
pp. 79-90 ◽  
Author(s):  
Tessie Nuñez
Keyword(s):  
In Vitro Culture ◽  
Culture Media ◽  
Iron Concentration ◽  
Culture Method ◽  
State University ◽  
Plant Genotype ◽  
Initial Culture ◽  
Semi Solid ◽  
Two Stages

Using a suitable medium for specific plant genotype greatly improves the efficiency of the in vitro culture method. The Visayas State University (VSU)-based National Coconut Research Center -Visayas (NCRC-V) evaluated the comparative suitability of the COGENT medium and VSU-modified Y3 (mY3) as in vitro culture media for coconut embryos using Albuera Dwarf (ALD), Baybay Tall (BAYT),and the VSU-developed Coconiño x Makapuno (VMAC1) hybrid. These two media differ in vitamin components, iron concentration and state during the first two stages of culture, namely germination and first subculture. Results showed that mY3 was more suitable for the in vitro germination and development of the coconut embryos than the COGENT medium. Significantly higher germination rates were observed in BAYT, ALD, and VMAC1 cultured in the semi-solid mY3 than those in the liquid COGENT medium from the first week until the fourth week of initial culture. Germination rates of 100%, 85.8% and 84.5% were obtained from CÑO x MAC, ALD, and BAYT, respectively. Furthermore, significantly higher percentages of germinating embryos with developing shoot and root were observed in the semi-solid mY3 than in the liquid COGENT. Likewise, better growth of plantlets in liquid mY3 was noted during the fourth and fifth months of culture. Among coconut types, VMAC1 had the highest germination rates in the two media and the best growth in mY3. BAYT had better growth in the COGENT medium while ALD had better shoot development in mY3.

scholarly journals In Vitro Culture Method for Accelerating Generation Turn-over in Pea.

1999 ◽  
Vol 68 (2) ◽  
pp. 402-408
Author(s):  
Tadashi Fujioka ◽  
Masayoshi Fujita ◽  
Yoshiki Miyamoto
Keyword(s):  
In Vitro Culture ◽  
Culture Method ◽  
Turn Over

scholarly journals Innovative Alternatives for Continuous In Vitro Culture of Babesia bigemina in Medium Free of Components of Animal Origin

Pathogens ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 343
Author(s):  
Jesús A. Álvarez Martínez ◽  
Julio V. Figueroa Millán ◽  
Massaro W. Ueti ◽  
Carmen Rojas-Martínez
Keyword(s):  
In Vitro Culture ◽  
Culture Media ◽  
Culture Method ◽  
Animal Origin ◽  
Acid Mixture ◽  
Babesia Bigemina ◽  
Lipid Mixture ◽  
Proliferation In Vitro ◽  
Animal Component

In this study, we report Babesia bigemina proliferation in culture medium free of components of animal origin supplemented with a lipid mixture. Babesia bigemina continuously proliferated in VP-SFM with a higher percent parasitized erythrocyte as compare to using other animal component-free culture media. Compared with Advanced DMEM/F12 (ADMEM/F12), VP-SFM had a similar percent parasitized erythrocyte (PPE). Supplementation of VP-SF with a lipid acid mixture improved B. bigemina proliferation in vitro culture, with a maximum PPE of 11.3%. Growth of B. bigemina in a perfusion bioreactor using VP-SFM medium supplemented with lipid mixture resulted in a PPE above 28%. In conclusion, we demonstrated that B. bigemina proliferated in an animal component-free medium supplemented with the fatty acid mixture. This innovation to B. bigemina in vitro culture method presented herein is an important source of biological material for live vaccine production and understanding the mechanisms and molecules involved in parasite attachment and invasion of bovine erythrocytes.

Development of in vitro culture method for zebrafish ovarian tissue fragment

Cryobiology ◽  
2011 ◽  
Vol 63 (3) ◽  
pp. 311-312 ◽  
Author(s):  
S. Anil ◽  
T. Zampolla ◽  
T. Zhang
Keyword(s):  
In Vitro Culture ◽  
Ovarian Tissue ◽  
Culture Method ◽  
Tissue Fragment
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