Diet of crab-eating fox (Cerdocyon thous) in two conservation units of the Amazon rainforest, Brazil

The present study aimed to evaluate the diet of the free-living crab-eating fox by identifying the stomach contents of the 17 crab-eating foxes (Cerdocyon thous) roadkilled in two conservation units, both located in the Amazon rainforest. The food items were quantified by frequency of occurrence (FO) and percentage of occurrence (PO). The stomach contents were analysed for dry matter (DM), crude protein (CP), crude fibre (CF), ether extract (EE), and mineral matter (MM). Nitrogen-free extractives (NFE), metabolisable energy (ME) values, as well as the energy need for maintenance were estimated. The composition of the diet for the crab-eating fox presented 29 food items from the different taxonomic groups, with a greater diversity of items of animal origin (n=22), although the highest frequency of occurrence was gramineae (Poaceae) (41.18%). Among the items of animal origin, 21% were mammals, 18% reptiles, 10% amphibians, 9% invertebrates and 3% birds. A high content of CF (62.76%) were determined. Nitrogen-free extractive and dry matter averages were 5.91% and 141.82 kcal/100g, respectively. The average maintenance energy was 447.01 kcal/day. These findings suggesting that the crab-eating foxes have a generalist diet with an omnivorous diet in the Amazon basin, feeding on gramineae, fruits, insects, snakes, amphibians, birds and small mammals and have the same feeding habit that present in other Brazilian biomes.

Food and Feeding Biology of Nile Tilapia (Oreochromis niloticus) in Lake Langeno, Ethiopia

This study aimed to investigate the natural feeding behavior of Nile tilapia in Lake Langeno, Ethiopia, with emphasis on potential spatial, size and seasonal effects on ingested food items. This study of the food and feeding biology of O. niloticus in Lake Langeno, Ethiopia, was conducted from March 2016to February 2017. Fish samples were collected monthly from six different sampling sites using different mesh sizes of gillnets. A total of 610 fish specimens with full stomachs were considered for the assessment of feeding biology. In total, seven food items, namely phytoplankton, zooplankton, insects, detritus, macrophytes, fish parts and nematodes, were identified from the fish stomach contents. Phytoplankton was the most commonly consumed food prey, followed by detritus, zooplankton and macrophytes. The other food items were occasionally and randomly consumed. Phytoplankton and detritus were the dominant food prey in the dry season, with zooplankton and macrophytes the main prey during the wet months. The contribution of phytoplankton, zooplankton and insects were slightly highest in small-sized groups (<10 cm), whereas detritus, macrophytes and fish parts were highest in larger-size groups (>20 cm) (p < 0.05). The present results point to a concurrence of the relative importance of dietary items at the individual level, species level and among the study sites. Phytoplankton was the primary consumed food item, which indicates the specialist feeding strategy of Nile tilapia in the lake. Generally, food items of plant origin, typically associated with less protein content than animal origin food items, dominated the stomach contents of Nile tilapia. The dietary pattern of Nile tilapia in Lake Langeno shifts with size and season, aspects that might warrant further study in view of aquaculture applications as well as climate change.

Prediction of Bioactive Peptides From Chicken Feather and Pig Hair Keratins Using In Silico Analysis Based on Fragmentomic Approach

Background: Keratin is among the most abundant structural proteins of animal origin, however it remains broadly underutilized. Objective: Bioinformatic investigation was performed to evaluate selected keratins originating from mass-produced waste products, i.e., chicken feathers and pig hair, as potential sources of bioactive peptides. Methods: Pepsin, trypsin, chymotrypsin, papain, and subtilisin were used for in silico keratinolysis with the use of “Enzyme(s) action” and fragmentomic analysis of theoretical products was performed using “Profiles of potential biological activity” in BIOPEP-UWM database of bioactive peptides. Bioactivity probability calculation and toxicity prediction of the peptides obtained were estimated using PeptideRanker and ToxinPred tools, respectively. Results: Our results showed that the keratins are a potential source of a variety of biopeptides, including dipeptidyl peptidase IV, angiotensin converting enzyme, prolyl endopeptidase inhibitory and antioxidative. Papain and subtilisin were found to be the most appropriate enzymes for keratin hydrolysis. This study presents possible structures of keratin-derived bioactive peptides that have not been previously described. Conclusion: Our data suggest additional in vitro and in vivo studies to verify theoretical predictions and further investigate the possibility of using keratin-rich waste as a source of peptide nutraceuticals.

A duplex real-time NASBA assay targeting serotype-specific gene for rapid detection of viable S. enterica serovar Paratyphi C in retail foods of animal origin

Salmonella enterica serovars Paratyphi C is highly adapted to humans and can cause a typhoid-like disease with high mortality rates. In this study, three serovar-specific genes were determined for S. Paratyphi C, SPC_0871，SPC_0872, and SPC_0908, by comparative genomics method. Based on SPC_0908 and xcd gene for testing Salmonella spp., we have developed a duplex real-time nucleic acid sequence-based amplification (real-time NASBA) with molecular beacon approach for simultaneous detection of viable cells of Salmonella spp. and serotype Paratyphi C. The test selectively and consistently detected 53 Salmonella spp. (representing 31 serotypes) and 18 non-Salmonella strains. Additionally, the method showed high resistance to interference by natural background flora in pork and chicken samples. The sensitivity of the established approach was determined to be 4.89 CFU/25 g in artificially contaminated pork and chicken samples after pre-enrichment. We propose this NASBA-based protocol as a potential detection method for Salmonella spp. and serotype Paratyphi C in food of animal origin.

Food Safety Governance and Guardianship: The Role of the Private Sector in Addressing the EU Ethylene Oxide Incident

Sesame seeds within the European Union (EU) are classified as foods not of animal origin. Two food safety issues associated with sesame seeds have emerged in recent years, i.e., Salmonella contamination and the presence of ethylene oxide. Fumigation with ethylene oxide to reduce Salmonella in seeds and spices is not approved in the EU, so its presence in sesame seeds from India was a sentinel incident sparking multiple trans-European product recalls between 2020–2021. Following an interpretivist approach, this study utilises academic and grey sources including data from the EU Rapid Alert System for Food and Feed (RASFF) database to inform a critical appraisal of current EU foods not of animal origin legislation and associated governance structures and surveillance programs. This is of particular importance as consumers are encouraged towards plant-based diets. This study shows the importance of collaborative governance utilizing data from company testing and audits as well as official regulatory controls to define the depth and breadth of a given incident in Europe. The development of reflexive governance supported by the newest technology (e.g., blockchain) might be of value in public–private models of food safety governance. This study contributes to the literature on the adoption of risk-based food safety regulation and the associated hybrid public–private models of food safety governance where both regulators and private organizations play a vital role in assuring public health.

Introduction to Special Issue: Study of Microbiological Safety in the Food Chain

Ensuring microbiological safety in the food (of animal origin) chain is a challenging task due to the complex interactions among animals, humans and the environment [...]

Biodegradable Composites with Functional Properties Containing Biopolymers

There is a major focus on natural biopolymers of bacterial, animal, or plant origin as ecological materials, replacing petrochemical products. Biologically derived polylactide (PLA), polyhydroxybutyrate (PHB), and polyhydroxyalkanoates (PHA) possess interesting properties, but they are currently too expensive for most applications. Therefore, researchers try to find other biopolymers that are both durable and cheap enough to replace plastics in some applications. One possible candidate is gelatin, which can be transformed into a thin, translucent film that is flexible and has stable and high mechanical properties. Here, we present a method of synthesizing a composite material from gelatin. For preparation of such material, we used gelatin of animal origin (pig skin) with the addition of casein, food gelatin, glycerin, and enzymes as biocatalysts of chemical modification and further extraction of gelatin from collagen. Compositions forming films with homogeneous shapes and good mechanical properties were selected (Tensile strength reaches 3.11 MPa, while the highest value of elongation at break is 97.96%). After administering the samples to microbial scaring, the composites completely decomposed under the action of microorganisms within 30 days, which proves their biodegradation.

Review of raw sources applicable for the development of plant-based products: Selection criteria for a balanced composition

Пищевые привычки и потребности в питательных веществах складываются на протяжении всей жизни человека под влиянием различных факторов, однако неоспоримым остается факт биологической потребности организма в незаменимых аминокислотах и как следствие - в доступных, полноценных белках. Поиск новых источников белка и развитие технологий его получения, воспроизводства, переработки, сбережения всегда в центре внимания пищевой промышленности. Потребность растущего населения Земли в доступных пищевых ресурсах, богатых полноценным белком, с каждым годом только возрастает. Выбор оптимальной формулы, способа переработки или сочетания композиции натуральных белков растительного происхождения, способных выступать в качестве возобновляемого источника незаменимых аминокислот, в целях полноценной замены белков животного происхождения, в частности молочных, в соответствии с принципами экономической целесообразности является основной движущей силой исследований и разработок. Актуальность обзора сырьевых источников и формирование критериев выбора растительного сырья с целью применения в моделях конструирования рецептур обоснованы стремительным ростом популярности продукции растительного происхождения. Безмолочные белковые напитки и десерты сформировали существенную потребительскую нишу, стали обязательной частью диеты современных потребителей не только комплементарно или в качестве перекуса, но и в качестве отдельного полноценного приема пищи, что формирует устойчивые привычки здорового пищевого поведения. Nutritional habits and needs for nutrients develop throughout a person's life under the influence of various factors, but the fact of the body's biological need for essential amino acids, and as a result, for accessible, complete proteins, remains undeniable. The search for new sources of protein and the development of technologies for its production, reproduction, processing, saving are always in the center of attention of the food industry. The demand of the growing world population for affordable food resources rich in complete protein only increases every year. The choice of the optimal formula, processing method or combination of a composition of natural proteins of plant origin, capable of acting as a renewable source of essential amino acids, in order to fully replace proteins of animal origin, and, in particular, dairy proteins, in accordance with the principles of economic feasibility, is the main driving force of research and development. The relevance of the review of raw materials and the formation of criteria for the selection of vegetable raw materials for the purpose of application in models for designing formulations is justified by the rapid growth in the popularity of products of vegetable origin. Dairy-free protein drinks and desserts have formed a significant consumer niche, have become an obligatory part of the diet of modern consumers, not only as a complementary or as a snack, but also as a separate full meal, which forms stable healthy eating habits.

Comparative Analysis of Mesenchymal Stem Cells Cultivated in Serum Free Media

Stem cells are attractive candidates for the regeneration of tissue and organ. Mesenchymal stem cells (MSCs) have been extensively investigated for their potential applications in regenerative medicine and cell therapy. For developing effective stem cell therapy, the mass production of consistent quality cells is required. The cell culture medium is the most critical aspect of the mass production of qualified stem cells. Classically, fetal bovine serum (FBS) has been used as a culture supplement for MSCs. Due to the undefined and heterologous composition of animal origin components in FBS, efforts to replace animal-derived components with non-animal-derived substances led to safe serum free media (SFM). Adipose derived mesenchymal stem cells (ADSCs) cultivated in SFM provided a more stable population doubling time (PDT) to later passage and more cells in a shorter time compared to FBS containing media. ADSCs cultivated in SFM had lower cellular senescence, lower immunogenicity, and higher genetic stability than ADSCs cultivated in FBS containing media. Differential expression analysis of mRNAs and proteins showed that the expression of genes related with apoptosis, immune response, and inflammatory response were significantly up-regulated in ADSCs cultivated in FBS containing media. ADSCs cultivated in SFM showed similar therapeutic efficacy in an acute pancreatitis mouse model to ADSCs cultivated in FBS containing media. Consideration of clinical trials, not only pre-clinical trial, suggests that cultivation of MSCs using SFM might offer more safe cell therapeutics as well as repeated administration due to low immunogenicity.

The Impact of Pre- and Probiotic Product Combinations on Ex vivo Growth of Avian Pathogenic Escherichia coli and Salmonella Enteritidis

Due to the global spread of antibiotic resistance, there is a strong demand to replace antimicrobial growth promotors in livestock. To identify suitable additives that inhibit the growth of avian pathogenic Escherichia coli O1/O18 and Salmonella enterica serotype Enteritidis strains, an ex vivo screening was performed. Inulin and fructooligosaccharides (FOS) were investigated as prebiotics. Enterococcus faecium and Bacillus coagulans served as probiotic strains. Firstly, the pathogen was anaerobically incubated in caecal digesta from different broiler breeder flocks with the addition of feed additives. Secondly, subsamples of these suspensions were incubated in an antibiotic medium for selective growth of the pathogen. During this step, turbidity was recorded, and lag times were calculated for each pathogen as readout of growth inhibition. Combinations of E. faecium with inulin or FOS significantly extended the lag time for E. coli compared to control. Moreover, older age was a significant factor to enhance this inhibitory effect. In contrast, the combination of FOS and B. coagulans showed shorter lag times for S. Enteritidis. Our results indicate that the E. faecium strain with prebiotics may inhibit the pathogen proliferation in the studied poultry flocks. Furthermore, our results suggest that prophylactic treatments should be assigned by feed additive, age and animal origin.