Adult human small intestine cell dissociation (on ice) v7 (protocols.io.rnnd5de)

protocols.io ◽  
2018 ◽  
Author(s):  
Andrew Potter
Keyword(s):  
Small Intestine ◽  
Cell Dissociation ◽  
Human Small Intestine ◽  
Adult Human ◽  
Intestine Cell

scholarly journals A proximal-to-distal survey of healthy adult human small intestine and colon epithelium by single-cell transcriptomics

2021 ◽  
Author(s):  
Joseph Burclaff ◽  
R. Jarrett Bliton ◽  
Keith A Breau ◽  
Meryem T Ok ◽  
Ismael Gomez-Martinez ◽  
...  
Keyword(s):  
Small Intestine ◽  
Single Cell ◽  
Drug Targets ◽  
Healthy Adult ◽  
Cellular Level ◽  
Cell Junction ◽  
Marker Genes ◽  
Human Small Intestine ◽  
Adult Human ◽  
Human Intestinal Epithelium

Background and Aims: Single-cell transcriptomics offer unprecedented resolution of tissue function at the cellular level, yet studies in healthy adult human small intestine and colon are sparse. Here, we present single-cell transcriptomics from 3 humans covering the duodenum, jejunum, ileum, and ascending, transverse, and descending colon. Methods: 12,590 single epithelial cells from three independently processed organ donors were evaluated for organ-specific lineage biomarkers, differentially regulated genes, receptors, and drug targets. Analyses focused on intrinsic cell properties and capacity for response to extrinsic signals along the gut axis across different humans. Results: Cells were assigned to 25 epithelial lineage clusters. Human intestinal stem cells (ISCs) are not specifically marked by many murine ISC markers. Lysozyme expression is not unique to Paneth cells (PCs), and PCs lack expression of expected niche-factors. BEST4 cells express NPY and show functional and maturational differences between SI and colon. Tuft cells possess a broad ability to interact with the innate and adaptive immune systems through previously unreported receptors. Some classes of mucins, hormones, cell-junction, and nutrient absorption genes show unappreciated regional expression differences across lineages. Differential expression of receptors and drug targets across lineages reveals biological variation and potential for variegated responses. Conclusions: Our study identifies novel lineage marker genes; covers regional differences; shows important differences between mouse and human gut epithelium; and reveals insight into how the epithelium responds to the environment and drugs. This comprehensive cell atlas of the healthy adult human intestinal epithelium resolves data gaps in anatomical regions along the gastrointestinal tract and advances our understanding of human intestinal physiology.

scholarly journals Detection and Characterization of Hemopoietic Stem Cells in the Adult Human Small Intestine

2006 ◽  
Vol 176 (9) ◽  
pp. 5199-5204 ◽  
Author(s):  
Lydia Lynch ◽  
Diarmuid O’Donoghue ◽  
Jonathan Dean ◽  
Jacintha O’Sullivan ◽  
Cliona O’Farrelly ◽  
...  
Keyword(s):  
Stem Cells ◽  
Small Intestine ◽  
Hemopoietic Stem Cells ◽  
Human Small Intestine ◽  
Adult Human

scholarly journals Laminins in the developing and adult human small intestine: Relation with the functional absorptive unit

2007 ◽  
Vol 236 (7) ◽  
pp. 1980-1990 ◽  
Author(s):  
Inga C. Teller ◽  
Joëlle Auclair ◽  
Elizabeth Herring ◽  
Rémy Gauthier ◽  
Daniel Ménard ◽  
...  
Keyword(s):  
Small Intestine ◽  
Human Small Intestine ◽  
Adult Human

Electron probe x-ray microanalysis and electron microscopy of amino acid absorption and transport by the small intestine: inhibition by chemical poisons and correlation to the elemental composition of the epithelial cells

1986 ◽  
Vol 44 ◽  
pp. 134-135
Author(s):  
A. J. Tousimis
Keyword(s):  
Small Intestine ◽  
Amino Acid ◽  
Epithelial Cells ◽  
Elemental Composition ◽  
Isotope Labeling ◽  
Structural Components ◽  
X Ray ◽  
Human Small Intestine ◽  
Transport Studies

The elemental composition of amino acids is similar to that of the major structural components of the epithelial cells of the small intestine and other tissues. Therefore, their subcellular localization and concentration measurements are not possible by x-ray microanalysis. Radioactive isotope labeling: I131-tyrosine, Se75-methionine and S35-methionine have been successfully employed in numerous absorption and transport studies. The latter two have been utilized both in vitro and vivo, with similar results in the hamster and human small intestine. Non-radioactive Selenomethionine, since its absorption/transport behavior is assumed to be the same as that of Se75- methionine and S75-methionine could serve as a compound tracer for this amino acid.

Bacterial flora of the human small intestine

JAMA ◽  
1966 ◽  
Vol 196 (13) ◽  
pp. 1125-1127 ◽  
Author(s):  
G. H. Bornside
Keyword(s):  
Small Intestine ◽  
Bacterial Flora ◽  
Human Small Intestine

Metabolism of Bile Salts by Alimentary Bacteria During Transit in the Human Small Intestine

1994 ◽  
Vol 8 (4) ◽  
Author(s):  
P. Marteau ◽  
M. F. Gerhardt ◽  
A. Myara ◽  
E. Bouvier ◽  
F. Trivin ◽  
...  
Keyword(s):  
Small Intestine ◽  
Bile Salts ◽  
Human Small Intestine
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