scholarly journals Evaluation of Trichoderma asperellum B1902 in Controlling Fusarium Wilt of Cavendish Banana Cultivar

2021 ◽  
Vol 50 (9) ◽  
pp. 2549-2561
Author(s):  
Sharifah Siti Maryam Syd Abdul Rahman ◽  
Nur Ain Izzati Mohd Zainudin ◽  
Nor Azwady Abd. Aziz
Keyword(s):  
Biological Control ◽  
Fusarium Wilt ◽  
Wilt Disease ◽  
Internal Transcribed Spacers ◽  
Plant Diseases ◽  
Dual Culture ◽  
Species Identity ◽  
Trichoderma Species ◽  
Culture Test ◽  
Fusarium Wilt Disease

Trichoderma species is one of the microorganisms with antagonistic properties as biological control agents. In the banana industry, Fusarium wilt disease caused by Fusarium oxysporum f. sp. cubense (Foc) has been practically managed using chemical pesticides that led to environmental disruptions, ineffective conditions and disease resistance. In preliminary study, T. asperellum gave better result compared to other species in inhibiting the growth of Foc in in vitro condition. Therefore, the aim of this study was to examine the effects of T. asperellum as a biological control of Fusarium wilt disease of banana. A total of 326 fungal isolates were isolated from soil samples obtained around Malaysia and identified as Trichoderma species based on phenotype characteristics. The species identity for the best candidates from dual culture test was confirmed based on internal transcribed spacers (ITS) and translation elongation factor 1 alpha (TEF-1α) sequence identity. In dual culture test, findings showed that three isolates with a high percentage inhibition of radial growth (PIRG) were observed in plates of T. asperellum isolates B1902 (84.85%), T2007 (77.78%) and C1667 (75.76%), which successfully inhibited the growth of F. oxysporum f. sp. cubense isolate 9888. Based on in vivo test, the best candidate was T. asperellum B1902 with lower disease severity index (DSI) value of 0.2 compared to the inoculated control with DSI of 3.6. As a conclusion, T. asperellum B1902 can be used as an alternative treatment in managing Fusarium wilt disease. Hence, future study should be focused on applying T. asperellum as a biocontrol agent in the field and controlling other plant diseases in the agricultural plantation.

scholarly journals BIOLOGICAL CONTROL OF FUSARIUM WILT DISEASE IN SWEET BASIL PLANT

2019 ◽  
Vol 48 (2) ◽  
pp. 67-84
Author(s):  
Sabah A. Mohamed ◽  
Nazmy A. Abd EL-Ghany ◽  
Mohamed G. A. Nada ◽  
Mohammed H. M. Ramadan
Keyword(s):  
Biological Control ◽  
Fusarium Wilt ◽  
Wilt Disease ◽  
Sweet Basil ◽  
Fusarium Wilt Disease

scholarly journals Biological Control of Fusarium Wilt Disease of Pigeonpea

2002 ◽  
Vol 18 (5) ◽  
pp. 279-283 ◽  
Author(s):  
Rajesh Singh ◽  
B.K. Singh ◽  
R.S. Upadhyay ◽  
Bharat Rai ◽  
Youn-Su Lee
Keyword(s):  
Biological Control ◽  
Fusarium Wilt ◽  
Wilt Disease ◽  
Fusarium Wilt Disease

scholarly journals INCREASING SOIL SUPPRESSIVITY TO FUSARIUM WILT OF BANANA THROUGH BANANA INTERCROPPING WITH Allium spp. (PENINGKATAN SUPRESIFITAS TANAH TERHADAP PENYAKIT LAYU FUSARIUM PISANG MELALUI PERTANAMAN TUMPANGSARI ANTARA PISANG DAN Allium spp.)

2016 ◽  
Vol 19 (1) ◽  
pp. 33
Author(s):  
Arif Wibowo ◽  
Aulia Rahman Alboneh ◽  
Medina Uli Alba Somala ◽  
Siti Subandiyah ◽  
Tony Pattison ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Fusarium Wilt ◽  
Soil Analysis ◽  
Wilt Disease ◽  
Plant Diseases ◽  
Fluorescein Diacetate ◽  
Control Treatment ◽  
Soil Suppressiveness ◽  
Horticultural Crops ◽  
Fusarium Wilt Disease

Fusarium wilt, caused by Fusarium oxysporum f.sp. cubense (Foc), is one of the most destructive diseases of banana and has spread in many plantation areas in Indonesia. Until today, the effective ways to control banana fusarium wiltdisease have not yet been found. Some studies indicated thatAllium spp. could be used to suppress plant diseases caused by Fusarium. Allium spp. are important horticultural crops which are generally cultivated in some areas in Indonesia. This research was conducted to determine the effect of several species ofAllium spp. intercropped with banana to improve soil suppressiveness against banana fusarium wilt disease. The results showed that up to 12 months after planting, from 3 species ofAllium spp. (A. tuberosum/ Chinese leek, A. fistulosum/ bunching onion, and A. cepa var. aggregatum/ shallot) intercropped with banana Ambon Kuning (AAA) cultivar, Chinese leek and shallot were able to suppress the incidence of fusarium wilt disease of banana by 46 and 33% respectively. Soil analysis on the rhizosphere of banana intercropped with Chinese leek and shallot had lower population of total Fusarium compared to the other treatments. Analysis of fluorescein diacetate (3’.6’-diacetylfluoerescein) or FDA also showed that total microbial activity in the rhizosphere of banana intercropped withAllium spp. was also lower compared to control treatment (without intercropping). The observation of the effect ofAllium spp. extracts on Foc showed that Allium spp. extracts were able to suppress the development of the colony and spore germination of Foc in vitro.Keywords: Allium spp., banana, fusarium wilt suppression, intercroppingLayu Fusarium, yang disebabkan oleh Fusarium oxysporum f.sp. cubense (Foc), merupakan salah satu penyakit tanaman pisang yang paling merusak dan telah tersebar di berbagai daerah di Indonesia. Sampai saat ini cara yangefektif untuk mengendalikan penyakit layu fusarium pisang belum ditemukan. Beberapa penelitian menunjukkan bahwa Allium spp. dapat dipergunakan untuk menekan penyakit tumbuhan yang disebabkan oleh Fusarium. Allium spp.adalah tanaman hortikultura penting yang banyak dibudidayakan di Indonesia. Penelitian ini dilakukan untuk mengetahui pengaruh beberapa spesiesAllium spp. yang ditumpangsarikan dengan tanaman pisang untuk meningkatkansupresifitas tanah dalam menekan penyakit layu fusarium pisang. Hasil pengamatan menunjukkan bahwa sampai 12 bulan setelah tanam, dari 3 spesiesAllium spp. (A. tuberosum/kucai, A. fistulosum/loncang, dan A. cepa var. aggregatum/bawang merah) yang ditumpangsarikan dengan pisang kultivar Ambon Kuning (AAA), kucai dan bawang merah mampu menekan insidensi penyakit layu fusarium pisang berturut-turut sebesar 46% dan 33%. Analisis tanah rhizosfer pisang yang ditumpang sari dengan kucai dan bawang merah menunjukkan populasi total Fusarium yang lebih rendah daripada perlakuan lainnya. Analisis fluorescein diacetate (3’.6’-diacetylfluoerescein) atau FDA juga menunjukkan bahwa aktivitas total mikrobia pada rhizosfer pisang yang ditumpang sari dengan Allium spp. lebih rendah jika dibandingkan dengan perlakuan kontrol (tanpa tumpang sari). Pengamatan pengaruh ekstrak Allium spp. terhadap Foc menunjukkan bahwa ekstrak Allium spp. mampu menekan perkembangan koloni dan perkecambahan spora Foc secara in vitro.Kata kunci: Allium spp., penekanan layu fusarium, pisang, tumpang sari

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