scholarly journals The Immunogenicity and Protective Efficacy of Bovine Herpesvirus 1 Glycoprotein D plus Emulsigen Are Increased by Formulation with CpG Oligodeoxynucleotides

2002 ◽  
Vol 76 (18) ◽  
pp. 9002-9010 ◽  
Author(s):  
X. P. Ioannou ◽  
P. Griebel ◽  
R. Hecker ◽  
L. A. Babiuk ◽  
S. van Drunen Littel-van den Hurk
Keyword(s):  
Immune Response ◽  
Neutralizing Antibodies ◽  
Protective Efficacy ◽  
Bovine Herpesvirus ◽  
Ammonium Bromide ◽  
Cpg Odn ◽  
Glycoprotein D ◽  
Bovine Herpesvirus 1 ◽  
Cpg Oligodeoxynucleotides ◽  
Herpesvirus 1

ABSTRACT The immunogenicity and protective efficacy of a bovine herpesvirus 1 (BHV-1) subunit vaccine formulated with Emulsigen (Em) and a synthetic oligodeoxynucleotide containing unmethylated CpG dinucleotides (CpG ODN) was determined in cattle. A truncated, secreted version of BHV-1 glycoprotein D (tgD) formulated with Em and CpG ODN at concentrations of 25, 2.5, or 0.25 mg/dose produced a more balanced immune response, higher levels of virus neutralizing antibodies, and greater protection after BHV-1 challenge compared to tgD adjuvanted with either Em or CpG ODN alone. In contrast, tgD formulated with Em and either 25 mg of a non-CpG ODN or another immunostimulatory compound, dimethyl dioctadecyl ammonium bromide, induced similar immunity and protection compared to tgD formulated with Em alone, a finding which confirms the immunostimulatory effect of ODN to be CpG motif mediated. Our results demonstrate the ability of CpG ODN to induce a strong and balanced immune response in a target species.

scholarly journals Immunogenicity of a Bovine Herpesvirus 1 Glycoprotein D DNA Vaccine Complexed with Bovine Neutrophil Beta-Defensin 3

2014 ◽  
Vol 22 (1) ◽  
pp. 79-90 ◽  
Author(s):  
Sarah Mackenzie-Dyck ◽  
Laura Latimer ◽  
Ethel Atanley ◽  
Jennifer Kovacs-Nolan ◽  
Sam Attah-Poku ◽  
...  
Keyword(s):  
Immune Responses ◽  
Dna Vaccine ◽  
Protective Efficacy ◽  
Bovine Herpesvirus ◽  
Glycoprotein D ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus 1 ◽  
Humoral Responses ◽  
Bovine Neutrophil

ABSTRACTProtective efficacy against bovine herpesvirus 1 (BoHV-1) has been demonstrated to be induced by a plasmid encoding bovine neutrophil beta-defensin 3 (BNBD3) as a fusion construct with truncated glycoprotein D (tgD). However, in spite of the increased cell-mediated immune responses induced by this DNA vaccine, the clinical responses of BoHV-1-challenged cattle were not reduced over those observed in animals vaccinated with the plasmid encoding tgD alone; this might have been because the vaccine failed to improve humoral responses. We hypothesized that an alternative vaccine design strategy that utilized the DNA vaccine pMASIA-tgD as a complex with BNBD3 might improve humoral responses while maintaining robust Th1-type cell-mediated responses. C57BL/6 mice were vaccinated with pMASIA-tgD complexed with 0, 0.01875, 0.1875, or 1.875 nmol of a stable synthesized analog of BNBD3 (aBNBD3). The best results were seen in mice immunized with the vaccine composed of pMASIA-tgD complexed to 0.1875 nmol aBNBD3. In this group, humoral responses were improved, as evidenced by increased virus neutralization, tgD-specific early IgG1, and later IgG2a titers, while the strong cell-mediated immune responses, measured based on specific gamma interferon (IFN-γ)-secreting cells, were maintained relative to pMASIA-tgD. Modulation of the immune response might have been due in part to the effect of BNBD3 on dendritic cells (DCs).In vitrostudies showed that murine bone marrow-derived DCs (BMDCs) pretreated with aBNBD3 were activated, as evidenced by CD11c downregulation, and were functionally mature, as shown by increased allostimulatory ability. Native, synthetic, and analog forms of BNBD3 were equally capable of inducing functional maturation of BMDCs.

scholarly journals Neutralizing Antibodies to Bovine Herpesvirus 1 in Reindeer

1982 ◽  
Vol 23 (4) ◽  
pp. 565-569
Author(s):  
C. Ek-Kommonen ◽  
P. Veijalainen ◽  
M. Rantala ◽  
E. Neuvonen
Keyword(s):  
Neutralizing Antibodies ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus 1

TLR activation, immune response and viral protection elicited in cattle by a commercial vaccine against Bovine Herpesvirus-1

Virology ◽  
2021 ◽  
Author(s):  
Kornuta Claudia Alejandra ◽  
Cheuquepán Felipe ◽  
Bidart Juan Esteban ◽  
Soria Ivana ◽  
Gammella Mariela ◽  
...  
Keyword(s):  
Immune Response ◽  
Bovine Herpesvirus ◽  
Bovine Herpesvirus 1 ◽  
Commercial Vaccine ◽  
Herpesvirus 1

scholarly journals Antibodies against Bovine herpesvirus 1 in dairy herds in the state of Espirito Santo, Brasil

Revista CERES ◽  
2014 ◽  
Vol 61 (2) ◽  
pp. 280-283 ◽  
Author(s):  
Marcus Rebouças Santos ◽  
Hanna Carolina Campos Ferreira ◽  
Marcos Antônio dos Santos ◽  
Giuliana Loreto Saraiva ◽  
Natália Filardi Tafuri ◽  
...  
Keyword(s):  
Dairy Cattle ◽  
Neutralizing Antibodies ◽  
Bovine Herpesvirus ◽  
The State ◽  
Serum Samples ◽  
Bovine Herpesvirus 1 ◽  
Espírito Santo ◽  
Herpesvirus 1 ◽  
Cattle Herds ◽  
Espirito Santo

Bovine herpesvirus 1 (BoHV-1) causes major losses in worldwide livestock, affecting the respiratory and reproductive tracts of bovine. In the past decades, the number of cases in Brazil has been gradually increasing. Therefore, it is important to assess the distribution of infection in different regions of the country. In the state of Espírito Santo (ES) the BoHV 1 infection rate in dairy cattle herds is unknown. Thus, the aim of this study was to detect neutralizing antibodies against BoHV-1 in serum samples from 1,161 non-vaccinated cows from 59 dairy cattle herds in 23 municipalities of the Metropolitan, North, Northwest and South macro-regions. The identification of seropositive cows was evaluated by the virus neutralization test. The results showed that of all serum samples evaluated 775 (66.75%) had neutralizing antibodies against BoHV-1. Moreover, all herds were found positive; however, the percentage of positive cows varied among regions; 49.06%, 62.15%, 67.21% and 80.04% for the Metropolitan, South, North and Northwest macro-regions, respectively. In this study, the results clearly indicate the dissemination of the viral agent in dairy cattle in the ES state, requiring the monitoring and control of diseases related to BoHV-1 infection.

scholarly journals Bovine herpesvirus 1 glycoprotein D expression in bovine upper respiratory tract mediated by a human adenovirus type 5

2004 ◽  
Vol 35 (6) ◽  
pp. 715-721 ◽  
Author(s):  
Sacha Gogev ◽  
Jean-Pierre Georgin ◽  
Fr�d�ric Schynts ◽  
Alain Vanderplasschen ◽  
Etienne Thiry
Keyword(s):  
Respiratory Tract ◽  
Bovine Herpesvirus ◽  
Human Adenovirus ◽  
Adenovirus Type ◽  
Upper Respiratory Tract ◽  
Glycoprotein D ◽  
Bovine Herpesvirus 1 ◽  
Upper Respiratory ◽  
Herpesvirus 1 ◽  
Adenovirus Type 5

Genetic diversity of 3′ region of glycoprotein D gene of bovine herpesvirus 1 and 5

Virus Genes ◽  
2014 ◽  
Vol 48 (3) ◽  
pp. 438-447 ◽  
Author(s):  
Carolina Kist Traesel ◽  
Mariana Sá e Silva ◽  
Marcelo Weiss ◽  
Fernando Rosado Spilki ◽  
Rudi Weiblen ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Bovine Herpesvirus ◽  
Glycoprotein D ◽  
Bovine Herpesvirus 1 ◽  
Herpesvirus 1
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