scholarly journals Viral infection of spermatozoa. Part 1. Hepatitis B virus and human papillomavirus (review)

2021 ◽  
Vol 21 (4) ◽  
pp. 12-19
Author(s):  
E. E. Bragina
Keyword(s):  
Human Papillomavirus ◽  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Viral Infection ◽  
Vertical Transmission ◽  
Assisted Reproductive Technologies ◽  
Reproductive Technologies ◽  
Papilloma Virus ◽  
B Virus ◽  
Natural Conception

Intragametal viral infection of spermatozoa can cause true vertical transmission of viruses through germ cells. Currently, human papilloma-virus, including oncogenic risk strains, and hepatitis B virus, have been detected in spermatozoa. The possibility of vertical transmission of hepatitis B virus and human papillomavirus has been proven.Intragametal infection of spermatozoa with viruses of human papillomavirus and hepatitis B virus leads to abnormalities in the development of the embryo and can cause spontaneous abortions both duringnatural conception and when using assisted reproductive technologies.The development of adequate methods for diagnosing an intragametal spermatozoa virus infection will make it possible to find out, at least in some patients, the cause of infertility and pregnancy abnormalities and apply appropriate antiviral therapy in preparation for natural conception or the use of assisted reproductive technologies.

Vertical transmission of hepatitis B virus despite maternal lamivudine therapy

The Lancet ◽  
2002 ◽  
Vol 359 (9316) ◽  
pp. 1488-1489 ◽  
Author(s):  
Syed N Kazim ◽  
Salma M Wakil ◽  
Luqman A Khan ◽  
Seyed E Hasnain ◽  
Shiv K Sarin
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Vertical Transmission ◽  
Lamivudine Therapy ◽  
B Virus

scholarly journals Correlation between Vertical Transmission of Hepatitis B Virus and the Expression of HBsAg in Ovarian Follicles and Placenta

PLoS ONE ◽  
2013 ◽  
Vol 8 (1) ◽  
pp. e54246 ◽  
Author(s):  
Minmin Yu ◽  
Qian Jiang ◽  
Xiaojun Gu ◽  
Lili Ju ◽  
Ying Ji ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Vertical Transmission ◽  
Ovarian Follicles ◽  
B Virus

scholarly journals A Sensitive and Rapid Assay for Investigating Vertical Transmission of Hepatitis B Virus via Male Germ Line Using EGFP Vector as Reporter

2008 ◽  
Vol 2008 ◽  
pp. 1-6 ◽  
Author(s):  
Mohamed Morsi M. Ahmed ◽  
Tian-Hua Huang ◽  
Qing-Dong Xie
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Vertical Transmission ◽  
Germ Line ◽  
Dna Recombination ◽  
Green Fluorescence ◽  
Rt Pcr ◽  
Male Germ Line ◽  
Rapid Assay ◽  
B Virus

Hepatitis B virus (HBV) constitutes a serious menace to man. DNA recombination and sequencing, interspecific in vitro fertilization, single-embryo PCR and RT-PCR were employed to establish a sensitive and rapid assay for exploring the vertical transmission of viruses via male germ line. Plasmid pIRES2-EGFP-HBs which expressed enhanced green fluorescent protein as reporter for the expression of hepatitis B virusSgene was successfully constructed and confirmed by PCR, EcoR I and Sal I digestion, and DNA sequencing. After exposure to the plasmid, human spermatozoa were used to fertilize with zona-free hamster ova. Two-cell embryos were collected and classified into group A with green fluorescence and group B without green fluorescence under fluorescence microscope. The results showed that HBs DNA positive bands were detected in the embryos with green fluorescence (PCR and RT-PCR) and positive control (PCR) indicating expression of pIRES2-EGFP-HBs, and not observed in the embryos without green fluorescence and negative controls (PCR and RT-PCR) indicating no pIRES2-EGFP-HBs in the cells. The advantages and application foreground of this assay for study on vertical transmission of viruses such as HCV, HIV, HPV, and SARS via germ line were discussed.

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