USING SSR MARKERS TO DISTINGUISH APPLE CULTIVARS

The present investigation entitled “Allelic diversity in apple germplasm for fruit quality and scab resistance using SSR markers” was conducted in division of plant biotechnology, SKUAST-K, Shalimar during the year 2018. In the present study, four SSR markers namely Md-ACS-1, Md-ACO-1, ALO-7 and AM-19 were used for screening of 40 apple cultivars presently under cultivation in SKUAST-K for quality and scab resistance. The results revealed that out of 40 cultivars evaluated, 3 were found homozygous (ACS-1-2/2), 17 were heterozygous (ACS-1-1/2) and 10 were homozygous (ACS-1-1/1), while as no amplification of ACS-1 was found for 10 cultivars. Similarly, for Md-ACO-1, 3 cultivars were found homozygous, 35 as heterozygous and 2 did not show any amplification. As far as presence of Vf gene conferring resistance against scab disease, ALO-7 amplified at 820bp and 570bp alleles, 570 bp fragment was observed in resistant genotypes and was absent in susceptible genotypes, while the 820 amplicon was observed in both resistant and susceptible genotypes. The markers AM19 led to the amplification of 520 bp fragment in few cultivars.

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Evaluation of genetic diversity and relatedness among apple cultivars using RAPD and SSR markers

Agricultural Research Journal ◽

10.5958/2395-146x.2016.00003.x ◽

2016 ◽

Vol 53 (1) ◽

pp. 19 ◽

Cited By ~ 1

Author(s):

Manju Modgil ◽

Pardeep Pathani ◽

Arjun Chauhan

Keyword(s):

Genetic Diversity ◽

Ssr Markers ◽

Apple Cultivars

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Genetic diversity in Swedish and Finnish heirloom apple cultivars revealed with SSR markers

Scientia Horticulturae ◽

10.1016/j.scienta.2013.07.040 ◽

2013 ◽

Vol 162 ◽

pp. 43-48 ◽

Cited By ~ 16

Author(s):

Larisa Garkava-Gustavsson ◽

Claid Mujaju ◽

Jasna Sehic ◽

Anna Zborowska ◽

Gunter M. Backes ◽

...

Keyword(s):

Genetic Diversity ◽

Ssr Markers ◽

Apple Cultivars

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Genetic diversity of old and local apple cultivars of North Caucasus from VIR collection based on SSR markers

Plant Genetics, Genomics, Bioinformatics, and Biotechnology (PlantGen2021) ◽

10.18699/plantgen2021-205 ◽

2021 ◽

Keyword(s):

Genetic Diversity ◽

Ssr Markers ◽

North Caucasus ◽

Apple Cultivars

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Molecular Characterization of Apple Cultivars in Japan by S-RNase Analysis and SSR Markers

Journal of the American Society for Horticultural Science ◽

10.21273/jashs.130.6.885 ◽

2005 ◽

Vol 130 (6) ◽

pp. 885-892 ◽

Cited By ~ 14

Author(s):

Kentaro Kitahara ◽

Shogo Matsumoto ◽

Toshiya Yamamoto ◽

Junichi Soejima ◽

Tetsuya Kimura ◽

...

Keyword(s):

Genetic Diversity ◽

Molecular Characterization ◽

Ssr Markers ◽

Maternal Parent ◽

Golden Delicious ◽

Apple Cultivars ◽

Paternal Parent

We examined the genetic diversity and relatedness among apple (Malus ×domestica Borkh.) cultivars in Japan. The 42 apple cultivars, including major cultivars in Japan, were divided into five groups based on SSR genotypes. Most economically important cultivars belong in three groups: Fuji-Delicious, Golden Delicious, and Jonathan groups, and their genetic backgrounds seemed to be narrow. We also investigated the parent-offspring relationships of nine apple cultivars. `Jonathan', `Fuji', and `Rero 11' were identified as the respective paternal parents of three cultivars described as having unknown paternal parents (i.e., `Akagi', `Ambitious', and `Hokuto'). `Starking Delicious', `Senshu', and `Golden Delicious', rather than `Ralls Janet', `Hatsuaki', and `Indo', seemed to be the paternal parents of `Kinsei', `Kiou', and `Mellow', respectively. `Carolina Red June' was excluded as a paternal parent of `Ranzan'. Both attributed parents of `Scarlet' (`Akane' and `Starking Delicious') were excluded, and it was suggested that `Fuji' was used as either a maternal or a paternal parent of `Scarlet'. `Jonathan' rather than `McIntosh' seems to be a maternal parent of `Yukari'.

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Microsatellite loci variability in apple cultivars developed at VIR

PROCEEDINGS ON APPLIED BOTANY GENETICS AND BREEDING ◽

10.30901/2227-8834-2021-3-151-158 ◽

2021 ◽

Vol 182 (3) ◽

pp. 151-158

Author(s):

A. V. Shlyavas ◽

A. A. Trifonova ◽

I. S. Chepinoga ◽

A. S. Sidnin ◽

K. V. Boris

Keyword(s):

Genetic Diversity ◽

Ssr Markers ◽

Collection Management ◽

Breeding Programs ◽

Rare Alleles ◽

Pcr Products ◽

Apple Cultivars ◽

Place Of Origin ◽

High Level ◽

Traits For Breeding

Background. Microsatellite (SSR) markers are now widely used both for studying genetic diversity in fruit crop collections, including apple (Malus × domestica Borkh.), and for genotyping individual accessions. The apple collection held by VIR, being the largest in Russia, contains cultivars bred at five experiment stations of VIR. These cultivars not only meet the requirements of horticulture in the region of their origin, but also are interesting as sources of valuable traits for breeding programs. However, these cultivars have not previously been studied using microsatellite markers. Materials and methods. A set of 10 SSR markers (CH02c02b, CH03d01, CH01f03b, CH02d08, CH02c02a, CH05e03, CH02c09, CHVf1, CH01h01, COL) was used for genotyping 35 apple cultivars developed at VIR and four parental forms. Fluorescently labeled PCR products were separated by capillary electrophoresis on ABI Prism 3130xl.Results. In 35 apple cultivars from VIR, 97 alleles were identified at ten studied loci. The number of alleles per locus varied from 5 (CH02c02b) to 17 (CH02c02a). The frequency of unique alleles was 26,8%, with 12,4% for rare alleles. Polymorphism information content (PIC) values varied from 0,49 (CH02c02b) to 0,91 (CH02c02a) and averaged 0,76. Expected and observed heterozygosity levels averaged 0,79 and 0,56, respectively. Cluster analysis did not reveal a clear division of cultivars according to the place of origin (experiment stations of VIR), but revealed clustering according to pedigrees.Conclusion. A high level of polymorphism was observed in 35 apple cultivars from VIR using ten SSR markers. The obtained data on the allelic composition of the studied cultivars can be used for their identification and contribute to more efficient collection management. Further studying and SSR genotyping of VIR’s apple collection and comparison with the data obtained in the presented work can help to identify the origin of cultivars with unknown pedigrees.

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PARENT IDENTIFICATION OF HUNGARIAN APPLE CULTIVARS USING SSR MARKERS

Acta Horticulturae ◽

10.17660/actahortic.2009.839.64 ◽

2009 ◽

pp. 471-477 ◽

Cited By ~ 3

Author(s):

I. Király ◽

M. Tóth ◽

A. Pedryc ◽

J. Halász ◽

T. Deák

Keyword(s):

Ssr Markers ◽

Apple Cultivars

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Parent Identification of Eight Apple Cultivars by S-RNase Analysis and Simple Sequence Repeat Markers

HortScience ◽

10.21273/hortsci.40.2.314 ◽

2005 ◽

Vol 40 (2) ◽

pp. 314-317 ◽

Cited By ~ 17

Author(s):

Kentaro Kitahara ◽

Shogo Matsumoto ◽

Toshiya Yamamoto ◽

Junichi Soejima ◽

Tetsuya Kimura ◽

...

Keyword(s):

Ssr Markers ◽

Simple Sequence Repeat ◽

Sequence Repeat ◽

Simple Sequence Repeat Markers ◽

Maternal Parent ◽

Golden Delicious ◽

Apple Cultivars ◽

Paternal Parent ◽

Simple Sequence

As the parents of the some of the apple cultivars were unknown and others were uncertain, we investigated the parent-offspring relationships of eight apple cultivars by S-RNase analysis and SSR markers. The paternal parent of `Hida' was identified as `Golden Delicious', not the previously mentioned `Orin'. It was indicated that `Ryoka No Kisetsu' and `Korin' showing identical SSR genotype are likely sports of `Fuji'. `Fuji', rather than `Toko', seemed to be a maternal parent of `Kotoku', but was not a paternal parent of `Orei', `Starking Delicious', `Nero 26', `Empire', or `Aori 3'. Previously mentioned `Mutsu', `Indo', and `Shin Indo' were excluded as paternal parents of `Hokuto'. `Tsugaru' and `Jonathan' and were identified as the respective paternal parents of three cultivars described as having unknown paternal parents, i.e., `Aika No Kaori', `Yoko', and `Tsugaru'.

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