scholarly journals Complement binding reaction in gonads transplant. I. M. Shustrov, G. A. Vasiliev (Moscow Medical Zh., 1926, No. 4)

2021 ◽  
Vol 22 (7) ◽  
pp. 864-864
Author(s):  
V. S.
Keyword(s):  
Binding Reaction

Research carried out in this direction by I. M. Shustrov G. A. Vasiliev (Moscow. Med. Zh., 1926, No. 4)

scholarly journals The Hematin-Binding Reaction as a Basis for Serum Albumin Determination

1952 ◽  
Vol 199 (2) ◽  
pp. 911-921
Author(s):  
Morris Rosenfeld ◽  
Douglas M. Surgenor
Keyword(s):  
Serum Albumin ◽  
Binding Reaction

scholarly journals Characterization of the adenosine deaminase-adenosine deaminase complexing protein binding reaction.

1990 ◽  
Vol 265 (31) ◽  
pp. 19312-19318
Author(s):  
W.P. Schrader ◽  
C.A. West ◽  
A.D. Miczek ◽  
E.K. Norton
Keyword(s):  
Protein Binding ◽  
Adenosine Deaminase ◽  
Binding Reaction

scholarly journals RUBELLA INFECTION OF SYNOVIAL CELLS AND THE RESISTANCE OF CELLS DERIVED FROM PATIENTS WITH RHEUMATOID ARTHRITIS

1970 ◽  
Vol 131 (2) ◽  
pp. 367-375 ◽  
Author(s):  
Arthur I. Grayzel ◽  
Carolyn Beck
Keyword(s):  
Fluorescence Intensity ◽  
Growth Stimulation ◽  
Cell Level ◽  
Pronounced Increase ◽  
Binding Reaction ◽  
Major Histocompatibility Locus ◽  
Histocompatibility Locus ◽  
Protein Moiety ◽  
Mechanism Of Growth

The mechanism of growth stimulation in allogeneic lymphocytes mixed in vitro was studied at the cell level by means of cytophotometric techniques. A pronounced increase in fluorescence intensity of fixed and acridine orange (AO) stained lymphocytes was observed as soon as after 1–3 hr in mixed culture. No increase in the amount of DNA took place during this time. The higher fluorescence intensity was due to an increased accessibility of AO binding sites in the deoxyribonucleoprotein (DNP) complex, most probably as a result of weakened bonds between the DNA and the protein moiety in the DNP complex. Similar DNP changes have been found in other systems of growth stimulation and may be one prerequisite for later induction of cellular synthetic processes. Increased AO binding only occurred when the lymphocyte donors were incompatible at the major histocompatibility locus (HL-A); there was no change in AO binding in cases of HL-A identity. The AO binding reaction probably reflects a specific recognition of HL-A antigens, whereas other antigenic discrepancies between the individuals do not seem to cause an analogous response.

scholarly journals A proline switch explains kinetic heterogeneity in a coupled folding and binding reaction

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Franziska Zosel ◽  
Davide Mercadante ◽  
Daniel Nettels ◽  
Benjamin Schuler
Keyword(s):  
Binding Reaction ◽  
Kinetic Heterogeneity

The Effect of Microwave Sintering Time to PM Aluminum Alloys

2015 ◽  
Vol 754-755 ◽  
pp. 240-244
Author(s):  
M.N. Derman ◽  
Syaza Nabilla Mohd Suhaimi ◽  
Zuraidawani Che Daud
Keyword(s):  
Microwave Sintering ◽  
Microwave Oven ◽  
Solid Particles ◽  
Binding Reaction ◽  
Aluminium Powder ◽  
Sintering Time ◽  
Conventional Sintering ◽  
Rapid Sintering ◽  
Powder Particles ◽  
Solid Liquid

Microwave sintering is new sintering technology method to produce Al alloys. The advantages of this method because of very short sintering time and less production cost compare to conventional sintering. However, the main problems in microwave sintering are required to be controlled sintering time due to rapid sintering mechanism. Therefore the effect of microwave sintering time to PM Aluminium will be studied. The compacted and sintered aluminium powder is placed in a microwave oven at a different period of 5 minutes, 10 minutes, 15 minutes and 20 minutes. Compression of 150 MPa is applied on aluminium powder to form pellets. Palette is shaped to 1cm in diameter and weighs 1g. SiC is placed together with aluminium samples in the microwave for the purpose of absorbing electromagnetic energy and is converted to heat. Results of different period sintering of aluminium pallet production altered physical properties of each sample. For a rapid sintering time, aluminium pallet does not show any binding reaction between powder particles. Whereas, for long microwave sintering period, solid particles phase change into solid-liquid phase caused by the movement and the formation of bonds between particles. Hence, this will be affecting the mechanical properties of the sample material.

Calorimetric study of the binding reaction of concanavalin a with immunoglobulins

1982 ◽  
Vol 19 (7) ◽  
pp. 907-911 ◽  
Author(s):  
Maria Dani ◽  
Fabrizio Manca ◽  
Giovanni Rialdi
Keyword(s):  
Concanavalin A ◽  
Calorimetric Study ◽  
Binding Reaction

scholarly journals Common cardiac medications potently inhibit ACE2 binding to the SARS-CoV-2 Spike, and block virus penetration into human lung cells

2021 ◽  
Author(s):  
Hung Caohuy ◽  
Ofer Eidelman ◽  
Tinghua Chen ◽  
Qingfeng Yang ◽  
Alakesh Bera ◽  
...  
Keyword(s):  
Cardiac Glycosides ◽  
Human Lung ◽  
Pseudotyped Virus ◽  
Target Cells ◽  
Lung Cells ◽  
Short Term ◽  
Binding Reaction ◽  
Human Lung Cells ◽  
Competitive Inhibitors ◽  
Repurposed Drugs

To initiate SARS-CoV-2 infection, the Receptor Binding Domain (RBD) on the viral spike protein must first bind to the host receptor ACE2 protein on pulmonary and other ACE2-expressing cells. We hypothesized that cardiac glycoside drugs might block the binding reaction between ACE2 and the Spike (S) protein, and thus block viral penetration into target cells. To test this hypothesis we developed a biochemical assay for ACE2:Spike binding, and tested cardiac glycosides as inhibitors of binding. Here we report that ouabain, digitoxin, and digoxin are high-affinity competitive inhibitors of ACE2 binding to the Wuhan S1 and the European [E614G] S1 proteins. These drugs also inhibit ACE2 binding to the Wuhan RBD, as well as to RBD proteins containing the S.Africa [E484K], Mink [Y453F] and UK [N501Y] mutations. As hypothesized, we also found that ouabain and digitoxin blocked penetration by SARS-CoV-2 Spike-pseudotyped virus into human lung cells. These data indicate that cardiac glycosides may block viral penetration into the target cell by first inhibiting ACE2:Spike binding. Clinical concentrations of ouabain and digitoxin are relatively safe for short term use for subjects with normal hearts. It has therefore not escaped our attention that these common cardiac medications could be deployed as inexpensive repurposed drugs for anti-COVID-19 therapy.

scholarly journals A high-affinity inositol 1,3,4,5-tetrakisphosphate receptor protein from brain is specifically labelled by a newly synthesized photoaffinity analogue, N-(4-azidosalicyl)aminoethanol(1)-1-phospho-d-myo-inositol 3,4,5-trisphosphate

1991 ◽  
Vol 280 (2) ◽  
pp. 533-539 ◽  
Author(s):  
G Reiser ◽  
R Schäfer ◽  
F Donié ◽  
E Hülser ◽  
M Nehls-Sahabandu ◽  
...  
Keyword(s):  
Molecular Mass ◽  
Anion Exchange ◽  
Protein Band ◽  
Anion Exchange Chromatography ◽  
Bovine Brain ◽  
Exchange Chromatography ◽  
Receptor Protein ◽  
Radioactive Label ◽  
Binding Reaction ◽  
High Affinity

A photolabile arylazido analogue of Ins(1,3,4,5)P4 selectively substituted at the 1-phosphate group was synthesized by coupling 2-aminoethanol(1)-1-phospho-D-myo-inositol 4,5-bisphosphate with N-hydroxysuccinimidyl-4-azidosalicylic acid [Schäfer, Nehls-Sahabandu, Grabowsky, Dehlinger-Kremer, Schulz & Mayr (1990) Biochem. J. 272, 817-825] and subsequently phosphorylating the product by bovine brain Ins(1,4,5)P3 3-kinase. The product, N-(4-azidosalicyl)-aminoethanol(1)-1-phospho-D-myo-inositol 3,4,5-trisphosphate [AsaIns(1,3,4,5)P4] was radioiodinated and purified by anion-exchange chromatography. AsaIns(1,3,4,5)P4 bound to a high-affinity Ins(1,3,4,5)P4 receptor from pig cerebellum with an affinity only 3-fold lower than that of Ins(1,3,4,5)P4. Photoirradiation of 125I-AsaIns(1,3,4,5)P4 in the presence of the receptor preparation revealed that the radioactive label was specifically associated with a protein band of apparent molecular mass 42 kDa, which Donié & Reiser [(1991) Biochem. J. 275, 453-457] had previously tentatively assigned to the Ins(1,3,4,5)P4 receptor protein. The radioactive label was displaced from the receptor when the binding reaction with 125I-AsaIns(1,3,4,5)P4 was carried out in the presence of 5 microM-Ins(1,3,4,5)P4.

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