scholarly journals Investigation on the bio-efficacy of fungal and bacterial bio-agents against Alternaria alternata inciting little millet leaf blight

2020 ◽  
Vol 34 (4) ◽  
pp. 251-257
Author(s):  
BODA PRAVEEN ◽  
K B. PALANNA ◽  
M K. PRASANNA KUMAR ◽  
A NAGARAJA ◽  
◽  
...  
Keyword(s):  
Alternaria Alternata ◽  
Leaf Blight ◽  
Little Millet

scholarly journals Redroot pigweed as a host for Alternaria alternata – the causal agent of Alternaria leaf blight in potato

2015 ◽  
Vol 102 (1) ◽  
pp. 115-118 ◽  
Author(s):  
Stanisław Mazur ◽  
Halina Kurzawińska ◽  
Małgorzata Nadziakiewicz ◽  
Jacek Nawrocki
Keyword(s):  
Alternaria Alternata ◽  
Leaf Blight ◽  
Causal Agent ◽  
Redroot Pigweed ◽  
Alternaria Leaf Blight

scholarly journals First report of Alternaria alternata causing leaf blight on little millet (Panicum sumatrense) in India.

Plant Disease ◽  
2020 ◽  
Author(s):  
Boda Praveen ◽  
A. Nagaraja ◽  
M. K. Prasanna Kumar ◽  
Devanna Pramesh ◽  
K. B. Palanna ◽  
...  
Keyword(s):  
Crop Yields ◽  
Disease Incidence ◽  
Leaf Blight ◽  
Post Inoculation ◽  
Pathogenicity Test ◽  
Conidial Suspension ◽  
Pcr Assay ◽  
Causal Organism ◽  
First Report ◽  
Little Millet

Little millet (LM) is a minor cereal crop grown in the Indian sub-continent. During October 2018, dark brown, circular to oval necrotic spots surrounded by concentric rings were observed on the upper leaf surface of the LM (cv. VS-13) grown in the fields of the University of Agricultural Sciences, Bengaluru, India (13.0784oN, 77.5793oE). As the disease progressed, infected leaves became blighted. Disease incidence up to 53% was recorded in 3 fields of 0.4-hectare area each. Thirty symptomatic leaves were collected to isolate the associated causal organism. The margins of diseased tissue were cut into 5 × 5-mm pieces, surface-sterilized in 75% ethanol for 45 seconds followed by 1% sodium hypochlorite for 1 min, finally rinsed in sterile distilled water five times and placed on PDA. After 7 days of incubation at 25°C, greyish fungal colonies appeared on PDA. Single-spore isolations were performed to obtain ten isolates. Pure cultures of the fungus initially produced light gray aerial mycelia that later turned to dark grey. All isolates formed obclavate to pyriform conidia measured 22.66-48.97μm long and 6.55-13.79µm wide with 1-3 longitudinal and 2-7 transverse septa with a short beak (2.55-13.26µm) (n=50). Based on the conidial morphology, the fungus was identified as Alternaria sp. Further, the taxonomic identity of all ten isolates was confirmed as A. alternata using species-specific primers (AAF2/AAR3, Konstantinova et al. 2002) in a PCR assay. Later, one of the isolate UASB1 was selected, and its internal transcribed spacer (ITS) region, glyceraldehyde-3-phosphate dehydrogenase (gapdh), major allergen Alt a 1 (Alt a 1), major endo-polygalacturonase (endoPG), OPA10-2, and KOG1058 genes were amplified in PCR (White et al. 1990; Berbee et al. 1999; Woudenberg et al. 2015), and the resultant products were sequenced and deposited in the NCBI GenBank (ITS, MN919390; gapdh, MT637185; Alt a 1, MT882339; endoPG, MT882340; OPA10-2, MT882341; KOG1058, MT882342). Blastn analysis of ITS, gapdh, Alt a 1, endoPG, OPA10-2, KOG1058 gene sequences showed 99.62% (with AF347031), 97.36% (with AY278808), 99.58% (with AY563301), 99.10% (with JQ811978), 99.05% (with KP124632) and 99.23% (with KP125233) respectively, identity with reference strain CBS916.96 of A. alternata, confirming UASB1 isolate to be A. alternata. For pathogenicity assay, conidial suspension of UASB1 isolate was spray inoculated to ten healthy LM (cv. VS-13) plants (45 days old) maintained under protected conditions. The spore suspension was sprayed until runoff on healthy leaves, and ten healthy plants sprayed with sterile water served as controls. Later, all inoculated and control plants were covered with transparent polyethylene bags and were maintained in a greenhouse at 28±2 ◦C and 90% RH. The pathogenicity test was repeated three times. After 8 days post-inoculation, inoculated plants showed leaf blight symptoms as observed in the field, whereas no disease symptoms were observed on non-inoculated plants. Re-isolations were performed from inoculated plants, and the re-isolated pathogen was confirmed as A. alternata based on morphological and PCR assay (Konstantinova et al. 2002). No pathogens were isolated from control plants. There is an increasing acreage of LM crop in India, and this first report indicates the need for further studies on leaf blight management and the disease impacts on crop yields.

Host range of Alternaria alternata f.sp. sphenocleae causing leaf blight of Sphenoclea zeylanica

1999 ◽  
Vol 77 (1) ◽  
pp. 103-112 ◽  
Author(s):  
Rhomela F. Masangkay ◽  
Maxima O. Mabbayad ◽  
Timothy C. Paulitz ◽  
Alan K. Watson
Keyword(s):  
Host Range ◽  
Alternaria Alternata ◽  
Leaf Blight

scholarly journals First Report of Alternaria alternata Causing Leaf Blight on Actinidia arguta in Liaoning, China

Plant Disease ◽  
2020 ◽  
Vol 104 (10) ◽  
pp. 2725
Author(s):  
Qin Wang ◽  
Qing-Shu Luan ◽  
Jian-Jun Wang ◽  
Xu Jiang ◽  
Gang Chen
Keyword(s):  
Alternaria Alternata ◽  
Leaf Blight ◽  
First Report ◽  
Actinidia Arguta

scholarly journals Prevalence and Severity of Leaf Blight Disease of Chrysanthemum in Southern regions of Karnataka and in-vitro Fungicidal Effect on Alternaria alternata

2015 ◽  
Vol 9 (3) ◽  
pp. 38-45 ◽  
Author(s):  
G. Shamala ◽  
G. R. Janardhana
Keyword(s):  
Alternaria Alternata ◽  
Disease Incidence ◽  
Life Sciences ◽  
Leaf Blight ◽  
Cut Flowers ◽  
In Vitro Evaluation ◽  
Karnataka State ◽  
Commercially Important ◽  
Blight Disease

Chrysanthemum is one of the commercially important flower crop with great potential both as loose and cut flowers in Karnataka state. In the present study, prevalence and incidence of leaf blight disease was studied in six major growing districts of South Karnataka during 2012 - 2013. The pathogen associated with leaf blight disease was isolated and identified as Alternaria alternata. The study revealed that, the disease was distributed in Southern districts of Karnataka wherever Chrysanthemum is grown. The varieties Chandini and Karnel were more susceptible to blight showing highest disease incidence (100%) and severity (100%) recorded from Mandya and Mysore regions. The lowest disease incidence, severity and PDI of 18.40%, 38.68% and 24.89% were recorded on Rajawhite variety in Bangalore region. During 2013, the highest disease incidence, severity and PDI with 100%, 98.47%, and 45.71% were recorded in Mysore district on Chandini variety. In vitro evaluation of four fungicides on Alternaria alternata revealed that Carbendazim+Mancozeb was most effective with 95.65% inhibition followed by Carbendazim, Mancozeb and Metalaxyl+Mancozeb with 68.26%, 91.30% and 91.30% inhibition of the mycelium at 2.0% concentration.DOI: http://dx.doi.org/10.3126/ijls.v9i3.12465   International Journal of Life Sciences 9 (3): 2015; 38-45

scholarly journals First Report of Leaf Blight Caused by Alternaria alternata on Trichosanthes kirilowii Maxim in China

Plant Disease ◽  
2016 ◽  
Vol 100 (5) ◽  
pp. 1021-1021
Author(s):  
L. X. Zhang ◽  
Y. R. Shi ◽  
J. H. Yu ◽  
H. Xu ◽  
G. J. Tan
Keyword(s):  
Alternaria Alternata ◽  
Leaf Blight ◽  
First Report ◽  
Trichosanthes Kirilowii
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