scholarly journals Optimizing Genomic DNA Isolation and PCR Amplification For Pasak Bumi (Eurycoma longifolia)

2019 ◽  
Vol 1 (2) ◽  
pp. 30
Author(s):  
Arida Susilowati ◽  
Henti Hendalastuti Rachmat ◽  
Ahmad Baiquni Rangkuti ◽  
Deni Elfiati ◽  
Ami Ambarwati
Keyword(s):  
Genomic Dna ◽  
Dna Isolation ◽  
Pcr Amplification ◽  
Eurycoma Longifolia ◽  
Genomic Dna Isolation

.

scholarly journals Optimizing the pure genomic DNA isolation procedure for Plectranthus amboinicus DNA – A prerequisite for further genomic Studies

2017 ◽  
Vol 2 (4) ◽  
Author(s):  
S. Manikandan ◽  
S. Ansarali ◽  
G.M. Alagu Lakshmanan
Keyword(s):  
Dna Barcoding ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Pcr Amplification ◽  
Leaf Tissue ◽  
Isolation Procedure ◽  
Modified Method ◽  
Genomic Dna Isolation ◽  
Young Leaves ◽  
Plectranthus Amboinicus

The DNA isolation procedure for different plant groups have been studied and standardized.  The isolation of pure genomic DNA is the most essential component for any type of molecular studies.  The present work is aimed to identify suitable DNA markers for the amplification of P.amboinicus  DNA  becomes a great hurdle  for DNA barcoding studies carried out by rbcL and matK primers Used in the members of Lamiaceae. To solve this problem, The DNA was extracted by three methods from fresh young leaf tissue of P.amboinicus. After the evaluationthe outcome of these methods, one most suitable modified method was selected for isolating DNA from young leaves of P.amboinicus and selected for suitable DNA barcoding markers for PCR amplification. The quality and quantity of DNAs are a prerequisite for genetic studies for a variety of plants including P.amboinicus. The quantity and quality of the DNA extracted by this method wasused for suitable DNA barcoding markers selection.

scholarly journals Comparative Analysis of the Genomic DNA Isolation Methods on Inula sp. (Asteraceae)

2016 ◽  
Vol 8 (4) ◽  
pp. 451-455
Author(s):  
Emre SEVİNDİK ◽  
Fatih COŞKUN ◽  
Zehra Tuğba MURATHAN ◽  
Mehmet Yavuz PAKSOY ◽  
Veysel UZUN
Keyword(s):  
Genomic Dna ◽  
Dna Isolation ◽  
Low Cost ◽  
Pcr Amplification ◽  
Isoamyl Alcohol ◽  
Dna Amount ◽  
Genomic Dna Isolation ◽  
Asteraceae Family ◽  
Positive Results ◽  
Commercial Kit

Simple, fast, low-cost and high throughput protocols are required for DNA isolation of plant species. In this study, phenol chloroform isoamyl alcohol and commercial (Sigma) DNA isolation kit methods were applied on some Inula species that belong to Asteraceae family. Genomic DNA amounts, A260, A280, A260/A230 and purity degrees (A260/A280) that were obtained through both methods were measured through electrophoresis and spectrophotometer. Additionally, PCR amplification was realized by primer pairs specific to nrDNA ITS, cpDNA ndhF (972F-1603R) and trnL-F regions. Results showed that maximum genomic DNA in nanograms obtained by phenol chloroform isoamyl alcohol method. The study also revealed that I. macrocephala had the maximum DNA and I. heterolepis had the minimum DNA amount. A260/A280 purity degrees showed that the highest and lowest purity in gDNAs obtained through phenol-choloform isoamyl alcohol method were in I.aucheriana and I. salicina, respectively. The highest and lowest purity degrees of gDNAs obtained through commercial kit was observed in I. fragilis and I. macrocephala samples, respectively. PCR amplification results showed that while band profiles of each three regions (ITS, trnL-F and ndhF) did not yield positive results in PCR amplifications using phenol-choloform isoamyl alcohol method; PCR band profiles obtained through commercial kit yielded positive results. As a result, it is fair to say that the relation of genomic DNA with PCR was found to be more efficient although the maximum amount of genomic DNA was obtained through phenol chloroform isoamyl alcohol method. 

scholarly journals Development of a Competent and Trouble Free DNA Isolation Protocol for Downstream Genetic Analyses in Glycine Species

2016 ◽  
Vol 4 (8) ◽  
pp. 700 ◽  
Author(s):  
Muhammad Amjad Nawaz ◽  
Faheem Shehzad Baloch ◽  
Hafiz Mamoon Rehman ◽  
Bao Le ◽  
Fahima Akther ◽  
...  
Keyword(s):  
Molecular Biology ◽  
Dna Extraction ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Plant Molecular Biology ◽  
Pcr Amplification ◽  
Cost Effective ◽  
Extraction Methods ◽  
Glycine Species ◽  
Genomic Dna Isolation

Extraction of deoxyribose nucleic acid (DNA) from plants is preliminary step in molecular biology. Fast and cost effective genomic DNA isolation from Glycine species for downstream application is a major bottleneck. Here we report a high throughput and trouble free method for genomic DNA extraction from leaf and seeds of Glycine species with high quality and quantity. Protocol reports the optimization by employing different concentrations of CTAB and PVP in extraction buffer. Efficiency of optimized protocol was compared with frequently used DNA extraction methods. Wide adoptability and utility of this protocol was confirmed by DNA extraction from leaves as well as seeds of G. max, G. soja, G. tomentella and G. latifolia. Extracted DNA was successfully subjected to PCR amplification of five microsatellite markers and four putative glycosyltransferase genes. DNA extraction protocol is reproducible, trouble free, rapid and can be adopted for plant molecular biology applications.

Genomic Dna Isolation From Plumbago L. Species Growing In India

2012 ◽  
Vol 2 (7) ◽  
pp. 45-46
Author(s):  
Varsha N Nathar ◽  
◽  
Prashant J Gadge
Keyword(s):  
Genomic Dna ◽  
Dna Isolation ◽  
Genomic Dna Isolation

scholarly journals Season, Environment Stress and Refrigerated Storage Affect Genomic DNA Isolation of Tung Tree

2012 ◽  
Vol 03 (11) ◽  
pp. 1562-1567
Author(s):  
Lingling Zhang ◽  
Yue Pan ◽  
Jinmin Fu ◽  
Junhua Peng
Keyword(s):  
Genomic Dna ◽  
Dna Isolation ◽  
Refrigerated Storage ◽  
Tung Tree ◽  
Genomic Dna Isolation ◽  
Environment Stress

Genomic DNA Isolation, Amplification of the D1S80 Locus and DNA-Gel Electrophoresis

2015 ◽  
pp. 42-42
Author(s):  
Arti Pandey ◽  
Arun Pandey ◽  
Naveen Shreevastava ◽  
Durga Neupane
Keyword(s):  
Gel Electrophoresis ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Genomic Dna Isolation ◽  
D1s80 Locus

Genomic DNA Isolation from Maize ( Zea mays ) Leaves Using a Simple, High‐Throughput Protocol

2016 ◽  
Vol 1 (1) ◽  
pp. 15-27 ◽  
Author(s):  
Kristen A. Leach ◽  
Paula C. McSteen ◽  
David M. Braun
Keyword(s):  
Zea Mays ◽  
High Throughput ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Genomic Dna Isolation

scholarly journals Chemically synthesized silver nanoparticles as cell lysis agent for bacterial genomic DNA isolation

2017 ◽  
Vol 8 (4) ◽  
pp. 045015 ◽  
Author(s):  
Gunajit Goswami ◽  
Himangshu Boruah ◽  
Trishnamoni Gautom ◽  
Dibya Jyoti Hazarika ◽  
Madhumita Barooah ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Genomic Dna ◽  
Dna Isolation ◽  
Cell Lysis ◽  
Genomic Dna Isolation

An effective protocol to solve the problem in genomic DNA isolation of tung tree

2013 ◽  
Vol 22 (4) ◽  
pp. 492-497 ◽  
Author(s):  
Lingling Zhang ◽  
Lijing Dai ◽  
Junbo Gou ◽  
Junhua Peng
Keyword(s):  
Genomic Dna ◽  
Dna Isolation ◽  
Tung Tree ◽  
Genomic Dna Isolation
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