DER P 5 FROM HOUSE DUST MITES HDM: EFFECT OF MUTATIONS AND HIGH TEMPERATURE ON THE FOLDING AND STABILITY OF THIS ALLERGEN, A POTENTIAL DIAGNOSTIC MARKER ALLERGEN FOR HOUSE DUST MITE ALLERGY

Twenty asthmatic children with prick tests positive for house dust or house dust mites were allocated to two groups that were matched for severity. One group was provided with zippered vinyl covers for pillows, mattresses, and box springs, and instructions for making the bedroom as easy to keep clean as a hospital ward; the other group was not. At the end of a 1-month study period, there was a marked and statistically significant difference in symptoms and signs of asthma between the two groups. Those with a dust-free bedroom had fewer days on which wheezing was observed, medication was given, or an abnormally low peak expiratory flow rate was recorded. Bronchial tolerance to aerosolized histamine significantly improved in the group whose bedrooms had been modified. A dust-free bedroom diminishes bronchial irritability and is a practical and effective method for decreasing asthma in children with house dust or house dust mite allergy.

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Isolation and characterization of the 68 kD allergen from house dust mite Dermatophagoides pteronyssinus

Journal of the Serbian Chemical Society ◽

10.2298/jsc0905513m ◽

2009 ◽

Vol 74 (5) ◽

pp. 513-522 ◽

Cited By ~ 1

Author(s):

Katarina Milovanovic ◽

Lidija Burazer ◽

Olga Vuckovic ◽

Marina Atanaskovic-Markovic ◽

Tanja Cirkovic-Velickovic ◽

...

Keyword(s):

Molecular Mass ◽

House Dust ◽

House Dust Mite ◽

Blot Analysis ◽

High Molecular Mass ◽

House Dust Mites ◽

House Dust Mite Allergy ◽

Ige Reactivity ◽

Mite Allergy ◽

Dust Mite

House dust mites (HDM) represent a major source of allergens, contributing to the increasing incidence of type I hypersensitivity disease worldwide. Over 30 different IgE-binding proteins from the HDM extract were detected. Although group 1 and 2 have been identified as major allergens, due to the safety and efficacy of allergy diagnosis and immunotherapy, there is a need to carefully evaluate the clinical relevance of other allergens present in the HDM extract. In regard to this, a high molecular mass allergen of about 68 kD was purified from the HDM extract using a combination of gel permeation chromatography and reversed-phase chromatography. The IgG and IgE reactivity of the purified protein were preserved during the purification process, as confirmed by Western blot analysis with polyclonal rabbit antibodies and dot blot analysis with a pool of sera from subjects with house dust mite allergy, respectively. In addition, the IgE reactivity was confirmed using ELISA testing with nine patient sera. The biological potency of the 68 kD allergen was confirmed by skin prick testing in five allergic subjects, suggesting that the high molecular mass allergen is a good candidate for component-resolved diagnosis of house dust mite allergy and eventual therapeutic treatment.

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Dust Mites

10.1071/9780643100497 ◽

2009 ◽

Cited By ~ 11

Author(s):

Matthew J Colloff

Keyword(s):

House Dust ◽

House Dust Mite ◽

Public Health Problem ◽

House Dust Mites ◽

Major Public Health Problem ◽

Dust Mites ◽

Important Species ◽

Mite Allergy ◽

Mite Control ◽

Dust Mite

Dust mites are present in almost every home – in our beds, clothing and carpets. Conservatively, at least 100 million people are affected by house dust mite allergy worldwide, manifesting itself as asthma, rhinitis or atopic dermatitis. Despite the growing recognition of this major public health problem, there is still no simple, effective, generally applicable strategy for dust mite control. Dust Mites incorporates for the first time in a single volume the topics of systematics and identification, physiology, ecology, allergen biochemistry and molecular biology, epidemiology, mite control and allergen avoidance. It explains key biological and ecological concepts for non-specialist readers, discusses ecological research methods and includes identification keys to dust mite species and life-cycle stage. It also explores how characteristics of population growth, water balance and physiology of dust mites have contributed to their importance as allergenic organisms. Many chapters contain new data, or new analyses of existing data, including global distribution maps of the most important species. Importantly, the book emphasises that studies of the biology and ecology of house dust mites should be regarded within the context of allergic disease rather than as ends in themselves, and that approaches to mite control in clinical management are subject to the same series of ecological rules as any other major problem in pest management. This comprehensive reference is essential reading for anyone involved or interested in house dust mite research and management.

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CONFIRMATION OF ETIOLOGIC DIAGNOSIS IN CHILDREN WITH HOUSE DUST MITE ALLERGY AS AN ESSENTIAL STAGE BEFORE ALLERGEN SPECIFIC IMMUNOTHERAPY. A REVIEW

Rossiiskii Allergologicheskii ZHurnal ◽

10.36691/rja287 ◽

2017 ◽

Vol 14 (6) ◽

pp. 98-106

Author(s):

O V Trusova ◽

A V Kamaev ◽

N L Lyashenko ◽

I V Makarova

Keyword(s):

Allergic Rhinitis ◽

House Dust ◽

House Dust Mite ◽

House Dust Mites ◽

Skin Tests ◽

Allergen Specific Immunotherapy ◽

House Dust Mite Allergy ◽

Mite Allergy ◽

Dust Mite

House dust mites are important cause for allergic rhinitis, and almost half of allergic rhinitis patients is sensitized to them. In patients with house dust mite allergy evidence of sensitivity can not be strictly achieved with medical history and patients complaints, since the cause-effect relationships are not obvious. At the same time incomplete allergological examination can lead to an incorrect definition of the main relevant allergens, and expensive treatment may not give the desired result in this cases. The estimation of the allergens for allergenspecific immunotherapy should be based on the disease history, detection of offending allergens by means of allergological examination in vivo with skin tests, nasal or conjunctival provocational tests, and component diagnostics if necessary. This approach based on use of provocational tests as a part of allergological examination is considered to be one of the ways to personalized therapy.

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