THE HISTORY, OPPORTUNITIES AND PROSPECTS OF TIME-LAPSE TECHNOLOGIES IN THE STUDY OF EARLY HUMAN EMBRYONIC DEVELOPMENT

Despite advances in assisted reproductive technologies, the high failure rate of existing stimulation protocols remains a key industry challenge. One of the leading reasons for this is the limited ability to assess the biological potential of the embryo and its chances of implantation. Over the past ten years, the focus of attention in reproductive technologies has significantly shifted from the patient to the embryo, since the need to improve their effectiveness stimulates the need to understand the deep processes of early development of the embryo. In order to increase the effectiveness of in vitro fertilization procedures in clinical embryology, high-tech methods of culturing and evaluating embryos are being introduced and improved. The purpose of the review is to demonstrate the history, possibilities and prospects in the study of early human embryonic development of time-lapse imaging technology. The active study and use of the capabilities of the time-lapse slow-motion technology allowed not only to expand the understanding of the processes of early development of the embryo, but also at the current moment allows us to assess its potential from the point of view of both biological and clinical perspectives. The main advantages of this method are the possibility of morphological assessment during the continuous cultivation of embryos in closed-type incubators without their extraction, as well as the determination of the exact time intervals of key events of the stages of embryo development with special attention to those moments that are not available for observation and fixation under conditions of traditional cultivation. clinical practice. The main point of growth for the development of time-lapse imaging technology was the creation and validation of the so-called morphokinetic criteria and algorithms for assessing the quality of developing embryos. The key perspective of the method is its use in combination with elements of artificial intelligence in order to predict the most potential embryo for transfer into the uterine cavity. Modern directions of research using the method of time-lapse shooting are the continuation of the development of morphokinetic algorithms and their effective criteria, the introduction of the technology of self-learning computer programs and the adaptation of these tools in clinical practice, the search and assessment of possible factors influencing the morphokinetics of embryos, quality control of the work of embryological laboratories. The future development of such technologies is presented in combination not only with the capabilities of artificial intelligence, but also in combination with the use of non-invasive genetic screening, the assessment of metabolomics and proteomics of developing embryos.

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Cleavage of Human Embryos: Options and Diversity

Acta Naturae ◽

10.32607/20758251-2016-8-3-88-96 ◽

2016 ◽

Vol 8 (3) ◽

pp. 88-96

Author(s):

Yu. K. Doronin ◽

I. V. Senechkin ◽

L. V. Hilkevich ◽

M. A. Kurcer

Keyword(s):

Time Lapse ◽

Reproductive Technologies ◽

Human Embryos ◽

Imaging Data ◽

Noninvasive Methods ◽

Topographic Features ◽

Time Parameters ◽

Embryo Cleavage ◽

Time Lapse Imaging ◽

Developmental Dynamics

In order to estimate the diversity of embryo cleavage relatives to embryo progress (blastocyst formation), time-lapse imaging data of preimplantation human embryo development were used. This retrospective study is focused on the topographic features and time parameters of the cleavages, with particular emphasis on the lengths of cleavage cycles and the genealogy of blastomeres in 2- to 8-cell human embryos. We have found that all 4-cell human embryos have four developmental variants that are based on the sequence of appearance and orientation of cleavage planes during embryo cleavage from 2 to 4 blastomeres. Each variant of cleavage shows a strong correlation with further developmental dynamics of the embryos (different cleavage cycle characteristics as well as lengths of blastomere cycles). An analysis of the sequence of human blastomere divisions allowed us to postulate that the effects of zygotic determinants are eliminated as a result of cleavage, and that, thereafter, blastomeres acquire the ability of own syntheses, regulation, polarization, formation of functional contacts, and, finally, of specific differentiation. This data on the early development of human embryos obtained using noninvasive methods complements and extend our understanding of the embryogenesis of eutherian mammals and may be applied in the practice of reproductive technologies.

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Assessment of the impact of asynchronous syngamy by time-lapse imaging on early human embryo development, implantation and live birth rates

10.26226/morressier.573c1511d462b80296c9836b ◽

2016 ◽

Author(s):

Shabana Sayed

Keyword(s):

Embryo Development ◽

Live Birth ◽

Human Embryo ◽

Time Lapse ◽

Birth Rates ◽

Live Birth Rates ◽

Human Embryo Development ◽

Time Lapse Imaging ◽

The Impact ◽

Early Human

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Time lapse imaging: is it time to incorporate this technology into routine clinical practice?

Human Fertility ◽

10.1080/14647273.2017.1283068 ◽

2017 ◽

Vol 20 (2) ◽

pp. 74-79 ◽

Cited By ~ 7

Author(s):

Priya Bhide ◽

Abha Maheshwari ◽

Rachel Cutting ◽

Susan Seenan ◽

Anita Patel ◽

...

Keyword(s):

Clinical Practice ◽

Time Lapse ◽

Routine Clinical Practice ◽

Time Lapse Imaging

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Does the transition from standard incubator to a time-lapse imaging chamber impact embryonic development and neonatal outcomes?

Fertility and Sterility ◽

10.1016/j.fertnstert.2015.07.987 ◽

2015 ◽

Vol 104 (3) ◽

pp. e315

Author(s):

R.W. Goldberg ◽

P.K. Gill ◽

J.M. Goldberg ◽

N. Desai

Keyword(s):

Embryonic Development ◽

Time Lapse ◽

Neonatal Outcomes ◽

Time Lapse Imaging

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Migration of primordial germ cells during late embryogenesis of pikeperch Sander lucioperca relative to blastomere transplantation

Czech Journal of Animal Science ◽

10.17221/40/2016-cjas ◽

2017 ◽

Vol 62 (No. 3) ◽

pp. 121-129 ◽

Cited By ~ 3

Author(s):

H. Güralp ◽

K. Pocherniaieva ◽

M. Blecha ◽

T. Policar ◽

M. Pšenička ◽

...

Keyword(s):

Embryonic Development ◽

Fluorescent Protein ◽

Primordial Germ Cells ◽

Primordial Germ Cell ◽

Somatic Cells ◽

Time Lapse ◽

Sander Lucioperca ◽

Initial Appearance ◽

Green Fluorescent ◽

Time Lapse Imaging

Pikeperch Sander lucioperca is a valuable fish in Europe, and basic information about its embryonic development, especially primordial germ cell (PGC) migration, is important for use in biotechnology. We categorized pikeperch embryonic development into six stages as in other fish species: zygote, cleavage, blastula, gastrula, segmentation, and hatching and described PGC migration. PGCs were visualized by injection of synthesized green fluorescent protein (GFP) within the 3’untranslated region (UTR) mRNA of nanos3. GFP-positive PGCs appeared in all embryos at approximately 100% epiboly. Time-lapse imaging revealed the PGC migration pattern from their initial appearance to location at the gonadal ridge. We conducted blastomere transplantation (BT) at the blastula stage. Donor embryos were labelled with GFP-nos3 3’UTR mRNA and tetramethylrhodamine dextran to label PGCs and somatic cells, respectively. Twelve BT chimeras were produced, with eight surviving to hatching. All exhibited donor-derived somatic cells in the developing body. The PGCs from donor embryos were observed to migrate towards the gonad region of the host embryos. Our results indicated that BT can be successfully applied in pikeperch, and these findings may be useful to produce germline chimeras in percids.

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46 Use of time-lapse imaging technology to assess relationships of morphological and phototextural attributes of presumptive ovine zygotes and early embryos with their developmental competence invitro

Reproduction Fertility and Development ◽

10.1071/rdv33n2ab46 ◽

2021 ◽

Vol 33 (2) ◽

pp. 130

Author(s):

S. Pena ◽

K. Fryc ◽

M. Murawski ◽

A. Nowak ◽

B. Kij ◽

...

Keyword(s):

Time Lapse ◽

Blastocyst Stage ◽

Developmental Competence ◽

Perivitelline Space ◽

Embryo Viability ◽

Pixel Intensity ◽

Developmental Potential ◽

Imaging Technology ◽

Time Lapse Imaging ◽

Zygote Stage

The assessment of morphology and digital image opacity may provide valuable information on embryo viability because such traits are linked to embryonic gene expression, metabolism and ultrastructure. Time-lapse imaging has been used in research to monitor the dynamic nature of the developing pre-implantation embryo, which includes capturing alterations in various morphological parameters over time. The present study examined the effectiveness of time-lapse technology in assessing several morphometric and phototextural parameters for predicting the developmental potential of ovine embryos. The development of 37 long wool sheep embryos from IVF to the blastocyst stage was monitored and evaluated using Primo Vision time-lapse imaging technology. Image-Pro Plus software was then used to measure zona pellucida thickness, embryo diameter, cellular grey-scale pixel intensity and heterogeneity, and total area of the perivitelline space. A one-way analysis of variance (ANOVA) was done using SigmaPlot® 11.0 for all attributes at various time points during embryo development [i.e. presumptive zygote stage, t(0); first cleavage, t(2) or t(3); second cleavage, t(4) or t(6); and third cleavage, t(7) or t(8)]. Our results indicate that most parameters analysed did not differ among embryos varying in their developmental fate, with the exception of the perivitelline space area, which was greater (P<0.05) for non-dividing embryos than for future blastocysts at the presumptive zygote stage (4040±4137 vs. 857±642µm2, respectively; mean±s.d.). Consequently, the measurement of perivitelline space at t(0) could be used to predict developmental potential of invitro-produced ovine embryos, but further investigation is required.

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In Vivo Time-Lapse Imaging of Zebrafish Embryonic Development

Cold Spring Harbor Protocols ◽

10.1101/pdb.prot4816 ◽

2007 ◽

Vol 2007 (8) ◽

pp. pdb.prot4816-pdb.prot4816 ◽

Cited By ~ 8

Author(s):

M. Distel ◽

R. W. Koster

Keyword(s):

Embryonic Development ◽

Time Lapse ◽

Time Lapse Imaging

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Advances in Imaging Technology of Anterior Segment of the Eye

Journal of Ophthalmology ◽

10.1155/2021/9539765 ◽

2021 ◽

Vol 2021 ◽

pp. 1-9

Author(s):

Sang Beom Han ◽

Yu-Chi Liu ◽

Karim Mohamed-Noriega ◽

Jodhbir S. Mehta

Keyword(s):

Artificial Intelligence ◽

Optical Coherence Tomography ◽

Clinical Practice ◽

Computer Science ◽

Anterior Segment ◽

Comprehensive Understanding ◽

New Techniques ◽

Ultrahigh Resolution ◽

Imaging Technology ◽

Precise Assessment

Advances in imaging technology and computer science have allowed the development of newer assessment of the anterior segment, including Corvis ST, Brillouin microscopy, ultrahigh-resolution optical coherence tomography, and artificial intelligence. They enable accurate and precise assessment of structural and biomechanical alterations associated with anterior segment disorders. This review will focus on these 4 new techniques, and a brief overview of these modalities will be introduced. The authors will also discuss the recent advances in research regarding these techniques and potential application of these techniques in clinical practice. Many studies on these modalities have reported promising results, indicating the potential for more detailed comprehensive understanding of the anterior segment tissues.

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