scholarly journals Response of Superovulation by Using FSH (Follicle Stimulating Hormone) and Sex Determination of Embryos Using PCR in Pesisir Cows of West Sumatra

2019 ◽  
Vol 20 (1) ◽  
pp. 7
Author(s):  
Tinda Afriani ◽  
Fery Lismanto Sayful ◽  
Sumedi Sumedi ◽  
Dino Eka Putra ◽  
Endang Purwati
Keyword(s):  
Drug Release ◽  
Sex Ratio ◽  
Sex Determination ◽  
Corpus Luteum ◽  
Follicle Stimulating Hormone ◽  
West Sumatra ◽  
Natural Mating ◽  
Stimulating Hormone

This study  was conducted to determine the response of superovulation by giving 16 ml dosage  of FSH hormone to female Pesisir cattle.  The estrus schedule of 15 Pesisir cows was set by inserting CIDR (Controlled Internal Drug Release) into the vagina for 12 days. At day 10, all cattles were injected with FSH for three consequent days but with decreasing dosage. On the 3rd day, FSH injection was accompanied by PGF2α injection and CIDR was removed. The detection of estrus was performed at  day 13. Natural mating was proceeded after the estrus signs visible. Collection of donor embryos was done on the 6th and 8th day after mating. The variables  measured were the response of superovulation, total number of corpus luteum, number of embryos and sex ratio. The results obtained were all  Pesisir cows responded to superovulation. The  average number of of corpus luteum and embryoes per cow were  5.93±3.17 and -----, respectively,  while the total of transferable embryoes were 90, with an average of 6.00 or 61.64%. The sexing of embryoes obtained in this study from 146 embryoes awere 76.03% males (111 embryoes) and 23.97% females (35 embryoes). Based on total of transferable embryoes, there were 51.37% male embryos and 11.28% of females embryos. The result of this study showed that the sex ratio of male embryos was higher than female embryos.

The reproductive habits and sex-determination of the nematode Rhabditis pellio Bütschli, with a note on its taxonomy and nomenclature

Parasitology ◽  
1953 ◽  
Vol 43 (1-2) ◽  
pp. 94-101 ◽  
Author(s):  
D. A. T. New
Keyword(s):  
Sex Ratio ◽  
Sex Determination ◽  
Natural Conditions ◽  
Separate Species ◽  
Chromosomal Arrangement ◽  
Parthenogenetic Reproduction ◽  
Rhabditis Pellio

1. The nematode Rhabditis pellio Bütschli may be cultured indefinitely on earthworm extract.2. Although the females of this nematode are usually capable of parthenogenetic reproduction, males are produced in the offspring only if copulation has taken place.3. Occasionally non-parthenogenetic generations appear but these do not represent a separate species.4. An explanation is given for the sex ratio found under natural conditions and it is suggested that sex-determination works on a chromosomal arrangement of XO = male and XX = female.5. The nematode named R. terrestris Stephenson 1942 is probably the same as that named R. pellio Bütschli by Johnson 1913.6. The nomenclature of R. pellio Bütschli is discussed.

LAPS-FSH: a new and effective long-acting follicle-stimulating hormone analogue for the treatment of infertility

2014 ◽  
Vol 26 (8) ◽  
pp. 1142 ◽  
Author(s):  
Sunyoung Jung ◽  
Youngjin Park ◽  
YoungHoon Kim ◽  
Yu Yon Kim ◽  
Hyun-Ji Choi ◽  
...  
Keyword(s):  
Ovarian Follicle ◽  
Follicle Stimulating Hormone ◽  
Ovarian Response ◽  
Pharmacokinetic Studies ◽  
Duration Of Treatment ◽  
Immunoglobulin G4 ◽  
Long Acting ◽  
Stimulating Hormone

Although several long-acting follicle-stimulating hormone (FSH) therapies have been developed to enhance the ovarian response, a disadvantage of FSH therapy is its relatively short half-life, which requires women to receive one to two injections per day for almost 2 weeks. In the present study, we developed a novel FSH analogue by conjugating recombinant human FSH (rhFSH) and the constant region of the human immunoglobulin G4 fragment via non-peptidyl linkers. The efficacy of the FSH analogue was evaluated in vitro by cAMP level assessments, pharmacokinetic studies and a determination of ovarian weight and by comparing these findings with the results from other FSH analogues. In addition, the total number of antral and Graafian follicles was determined after 7 days of treatment with control, 6 µg kg–1 follitropin β, 6, 12 or 42 µg kg–1 corifollitropin α or 3, 6 or 12 µg kg–1 long acting protein/peptide discovery-follicle-stimulating hormone (LAPS-FSH). As a result, the animals treated with 12 µg kg–1 LAPS-FSH produced additional and larger healthy follicles. These data demonstrate that LAPS-FSH promotes growth and inhibits atresia of the ovarian follicle compared with other available drugs, suggesting that our new drug enhances the efficacy and duration of treatment. It is expected that our new FSH analogue will result in a higher chance of pregnancy in patients who are unresponsive to other drugs.

scholarly journals The fertility of mice selected for large or small body size

1960 ◽  
Vol 1 (3) ◽  
pp. 393-407 ◽  
Author(s):  
Ruth E. Fowler ◽  
R. G. Edwards
Keyword(s):  
Body Weight ◽  
Body Size ◽  
Small Body ◽  
Follicle Stimulating Hormone ◽  
Weight Ratio ◽  
Total Body Weight ◽  
Control Line ◽  
Small Body Size ◽  
Natural Mating ◽  
Stimulating Hormone

The fertility of two unrelated strains of mice (strains N and C) which had both been selected for large and small body size has been studied.The fertility of pairs of mice in the large or small lines of strain C was unimpaired by selection. In strain N, some of the pairs in the large and small lines, but not in the control line, were sterile. Sterility in the large line was due to the low libido of the males, and not to female infertility. Sterility in the small line was probably due to hypo-functioning of the anterior pituitary of some females: the oestrous cycle was delayed or absent, some mice failed to ovulate after mating, and a high proportion of those mating had no implanted embryos at 12 days' gestation. Oestrus and ovulation could be induced in mice of the small line of strain N by exogenous gonadotrophins, and the proportion of mice with implanted embryos was considerably increased by progesterone supplements.The number of eggs found after natural mating was considerably higher in large mice than in small mice, and was significantly correlated with body weight in the small line of strain C only. The egg-number/body-weight ratio was higher in the lines of strain C than in those of strain N, though the ratios were similar when carcass fat was subtracted from total body weight.The amount of endogenous follicle-stimulating hormone secreted by the mice of the five lines was estimated by inducing ovulation with various amounts of exogenous gonadotrophins, and comparing the number of eggs found after each dose with the mean number ovulated after natural mating. Estimates of the amount of follicle-stimulating hormone secreted by mice of strain C were higher than those for mice of strain N.Differences in the rates of growth and in the numbers of eggs ovulated after natural mating indicate a higher level of pituitary activity in strain C than in strain N.

Sex Ratio and Sex Determination of Immature Brown-Headed Cowbirds

Bird-Banding ◽  
1976 ◽  
Vol 47 (2) ◽  
pp. 112 ◽  
Author(s):  
Richard A. Hill
Keyword(s):  
Sex Ratio ◽  
Sex Determination
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