scholarly journals Gold Nanoparticles Radio-sensitize and Reduce Cell Survival in Lewis Lung Carcinoma

2020 ◽  
Author(s):  
Arvind Pandey ◽  
Veronica Vighetto ◽  
Nicola Di Marzio ◽  
Matteo Hirsch ◽  
Nicola Ferrante ◽  
...  
Keyword(s):  
Dna Damage ◽  
Particle Size ◽  
Gold Nanoparticles ◽  
Cell Survival ◽  
Lung Carcinoma ◽  
Lewis Lung Carcinoma ◽  
Lewis Lung ◽  
Lung Carcinoma Cells ◽  
Reduce Cell Survival

It has been suggested that particle size plays an important role in determining the genotoxicity of gold nanoparticles (GNPs). The purpose of this study was to compare the potential radio-sensitization effects of two different sized GNPs (3.9 and 37.4 nm) fabricated and examined in vitro in Lewis Lung carcinoma (LLC) as a model of non-small cell lung cancer through use of comet and clonogenic assays. After the treatment of 2Gy X-ray irradiation, both particle sizes demonstrated increased DNA damage when compared to treatment with particles only and radiation alone. This radio-sensitization was further translated into a reduction in cell survival demonstrated by clonogenicity. This work indicates that GNPs of both sizes induce DNA damage in LLC cells at the tested concentrations, whereas the 37.4 nm particle size treatment group demonstrated greater significance in vitro. The presented data aids in the evaluation of the radiobiological response of Lewis Lung carcinoma cells treated with gold nanoparticles.

scholarly journals Gold Nanoparticles Radio-Sensitize and Reduce Cell Survival in Lewis Lung Carcinoma

Nanomaterials ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1717
Author(s):  
Arvind Pandey ◽  
Veronica Vighetto ◽  
Nicola Di Marzio ◽  
Francesca Ferraro ◽  
Matteo Hirsch ◽  
...  
Keyword(s):  
Dna Damage ◽  
Particle Size ◽  
Gold Nanoparticles ◽  
Cell Survival ◽  
Lung Carcinoma ◽  
Lewis Lung Carcinoma ◽  
Lewis Lung ◽  
Lung Carcinoma Cells ◽  
Reduce Cell Survival

It has been suggested that particle size plays an important role in determining the genotoxicity of gold nanoparticles (GNPs). The purpose of this study was to compare the potential radio-sensitization effects of two different sized GNPs (3.9 and 37.4 nm) fabricated and examined in vitro in Lewis lung carcinoma (LLC) as a model of non-small cell lung cancer through use of comet and clonogenic assays. After treatment with 2Gy X-ray irradiation, both particle sizes demonstrated increased DNA damage when compared to treatment with particles only and radiation alone. This radio-sensitization was further translated into a reduction in cell survival demonstrated by clonogenicity. This work indicates that GNPs of both sizes induce DNA damage in LLC cells at the tested concentrations, whereas the 37.4 nm particle size treatment group demonstrated greater significance in vitro. The presented data aids in the evaluation of the radiobiological response of Lewis lung carcinoma cells treated with gold nanoparticles.

scholarly journals Effect of Micelle-Incorporated Cisplatin With Sizes Ranging From 8 to 40 nm for the Therapy of Lewis Lung Carcinoma

2021 ◽  
Vol 12 ◽  
Author(s):  
Zhicheng Wang ◽  
Yumin Li ◽  
Tong Zhang ◽  
Hongxia Li ◽  
Zhao Yang ◽  
...  
Keyword(s):  
Block Copolymers ◽  
Lung Carcinoma ◽  
Lewis Lung Carcinoma ◽  
Drug Loading ◽  
Complexation Reaction ◽  
Lewis Lung ◽  
Antitumor Effects ◽  
Lung Carcinoma Cells

Insufficient transport of therapeutic cargo into tumor bed is a bottleneck in cancer nanomedicine. Block copolymers are promising carriers with smaller particle size by ratio modification. Here, we constructed cisplatin nanoparticles with sizes ranging from 8 to 40 nm to study the permeability and therapy of Lewis lung carcinoma. We synthesized methoxypoly(ethylene glycol)2000-block poly(L-glutamic acid sodium salt)1979 loading cisplatin through complexation reaction. The cisplatin nanomedicine has high drug loading and encapsulation efficiency. In vitro data demonstrated that cisplatin nanoparticles had equivalent growth-inhibiting effects on Lewis lung carcinoma cells compared to free cisplatin. In vivo evidences showed cisplatin nanoparticles had superior antitumor effects on the Lewis lung carcinoma mouse model with no obvious side effects. All results indicated that optimizing the ratio of block copolymers to obtain smaller sized nanomedicine could act as a promising strategy for overcoming the inadequate accumulation in poorly vascularized tumors.

DMNQ S-53 induces apoptosis and inhibits the growth of Lewis lung carcinoma cells in vitro and in vivo

2006 ◽  
Vol 1 (1) ◽  
pp. 73-79 ◽  
Author(s):  
Jae-Ho Lee ◽  
Eun-Ok Lee ◽  
Hyo-Jung Lee ◽  
Kwan-Hyun Kim ◽  
Kyoo-Seok Ahn ◽  
...  
Keyword(s):  
Lung Carcinoma ◽  
Lewis Lung Carcinoma ◽  
Carcinoma Cells ◽  
Lewis Lung ◽  
Lung Carcinoma Cells ◽  
Lewis Lung Carcinoma Cells

scholarly journals The effect of 2D tungsten disulfide nanoparticles on Lewis lung carcinoma cells in vitro

RSC Advances ◽  
2021 ◽  
Vol 11 (27) ◽  
pp. 16142-16150
Author(s):  
D. L. Kolesnik ◽  
O. N. Pyaskovskaya ◽  
O. P. Gnatyuk ◽  
V. V. Cherepanov ◽  
S. O. Karakhim ◽  
...  
Keyword(s):  
Lung Carcinoma ◽  
Lewis Lung Carcinoma ◽  
Cytotoxic Effect ◽  
Carcinoma Cells ◽  
Lewis Lung ◽  
Cytostatic Effect ◽  
Lung Carcinoma Cells ◽  
Lewis Lung Carcinoma Cells ◽  
Blue Spectral Region

WS2 2D nanoparticles show no cytotoxic and/or cytostatic effect on Lewis lung carcinoma cells after one day incubation. Only after two days incubation we registered cytotoxic effect. Cells incubated with 2D WS2 nanoparticles have luminescence in the blue spectral region.

Photodynamic anti-tumor activity of a new chlorin-based photosensitizer against Lewis Lung Carcinoma cells in vitro and in vivo

2003 ◽  
Vol 07 (03) ◽  
pp. 155-161 ◽  
Author(s):  
Dae-Seog Lim ◽  
Si-Hwan Ko ◽  
Dong-Hoon Won ◽  
Chang-Hee Lee ◽  
Won-Young Lee
Keyword(s):  
Lung Carcinoma ◽  
Lewis Lung Carcinoma ◽  
Biological Significance ◽  
Light Irradiation ◽  
Lewis Lung ◽  
Lung Carcinoma Cells ◽  
Anti Tumor Activity ◽  
Lewis Lung Carcinoma Cells

A biological significance of photodynamic therapy (PDT) with a new porphyrin derivative, DH-I-180-3 (Max. Abs. 666 nm), was examined. Experimental PDT with DH-I-180-3 against Lewis Lung Carcinoma 1 (LLC1) cells was designed in vitro and in vivo. For the comparison, PDT with an established photosensitizer, Photofrin®, was done. When the cells were treated with DH-I-180-3 (1.0 μg/ml) in vitro, the cells became fatally susceptible to the light (1.2 J/cm2) as early as in 1 h. All of these cells were irreversibly damaged in 24 h after light irradiation and categorized as necrosis. These were not seen in cells treated with Photofrin® for more than 4 h and remained unharmed by the light until the end of experiments. Mice (C57BL/6J) bearing LLC1 tumor were treated (intravenously) with DH-I-180-3 (400 to 800 μg/kg) or with Photofrin® (2 mg/kg) for 4 h. Following the light irradiation (1.2 J/cm2), retarded tumor growth was significant in mice treated with DH-I-180-3 compared with those treated with Photofrin®/PDT. Survival of mice receiving DH-I-180-3/PDT was prolonged approximately 30% and 40% compared with that of mice in a Photofrin® group. In conclusion, DH-I-180-3 absorbs a longer light wavelength, and instantaneously accumulates in tumor cells to make them susceptible to the light. In mice, a significantly low dose of DH-I-180-3 as little as 400 μg/kg was sufficient to produce a successful PDT result. Thus, we conclude that DH-I-180-3 is an effective photosensitizer to be developed.

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